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شناسایی چندشکلی جایگاه ژن دخیل در تنش گرمایی (HSP90β) در مرغهای بومی مرندی و تجاری | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 12، شماره 1 - شماره پیاپی 45، شهریور 1402، صفحه 1-8 اصل مقاله (364.85 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2023.65118.1884 | ||
| نویسندگان | ||
| جعفر پیش جنگ آقاجری* 1؛ قادر نجفی2؛ پارسا پیش جنگ آقاجری3؛ فاطمه محمدقلی فامیان3؛ الناز مهری4 | ||
| 1گروه علوم دامی، واحد مراغه، دانشگاه آزاد اسلامی، مراغه، ایران | ||
| 2گروه پاتوبیولوژی، دانشکده ی دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران | ||
| 3گروه علوم پایه، واحد بناب، دانشگاه آزاد اسلامی، بناب، ایران | ||
| 4گروه علوم پایه، واحد مراغه، دانشگاه آزاد اسلامی، مراغه، ایران | ||
| چکیده | ||
| تنش گرمایی زیانهای اقتصادی قابل توجهی را در تولید طیور بهوجود میآورد و منجر به کاهش چندین عامل فیزیولوژیکی و متابولیکی میشود. این تحقیق به منظور بررسی چندشکلی‌های آللی و ژنوتیپی ژن دخیل در تنش گرمایی (HSP90β) در مرغهای بومی مرندی، گوشتی و تخمگذار با استفاده از تکنیک PCR-RFLP انجام شد. بهطور تصادفی از 300 قطعه مرغ خونگیری و DNAی ژنومی به روش شستشوی نمکی استخراج شد. تکثیر جایگاه ژنی مورد نظر به طول 494 جفت باز به کمک آغازگرهای اختصاصی انجام و برای شناسایی جهش در جایگاه ژنی مورد نظر از آنزیم برشی MspI استفاده شد. بعد از هضم آنزیمی، برای جایگاه نشانگری HSP90β، دو نوع ژنوتیپ M1M1 و M1M2 و دو آلل M1 (با یک نوار 494 جفت بازی) و M2 (با دو نوار 248 جفت بازی و 246 جفت بازی) شناسایی شد. تودههای مرغهای بومی مرندی و گوشتی از نظر شاخص تعادل برای جایگاه ژنی مورد نظر در تعادل هاردی - واینبرگ قرار داشتند. برای تودههای مرغهای بومی مرندی و گوشتی شاخص اطلاعات شانون به ترتیب 25/0 و 40/0، شاخص تثبیت به ترتیب 07/0- و 16/0- و شاخص هتروزیگوسیتی مشاهده شده به ترتیب 14/0 و 28/0 برآورد شدند. با توجه به وجود چندشکلی و جهش در جایگاه ژنی مورد مطالعه میتوان در مرغهای بومی مرندی و گوشتی با انتخاب ژنتیکی با کمک این نشانگر ژنی، در حذف مرغهای حساس به گرما و نگهداری مرغهای مقاوم در برابر گرما استفاده شود. | ||
| کلیدواژه‌ها | ||
| اصلاح نژاد ژنتیکی؛ تنوع ژنوتیپی؛ شوک حرارتی؛ مرغ؛ نشانگر ژنی | ||
| عنوان مقاله [English] | ||
| Identification of gene locus polymorphism involved in heat stress (HSP90β) in Marandi indigenous and commercial chickens | ||
| نویسندگان [English] | ||
| Jafar Pish Jang Aghajeri1؛ Ghader Najafi2؛ Parsa Pish Jang Aghajari3؛ Fatemeh Mohammad Goli Famiyan3؛ Elnaz Mehri4 | ||
| 1Department of Animal Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary, Urmia Branch, Islamic Azad University, Urmia, Iran | ||
| 3Department of Basic Sciences, Bonab Branch, Islamic Azad University, Bonab, Iran | ||
| 4Department of Basic Sciences, Maragheh Branch, Islamic Azad University, Maragheh, Iran | ||
| چکیده [English] | ||
| Heat stress causes significant economic losses in poultry production and leads to the reduction of several physiological and metabolic factors. This research was conducted in order to investigate the allelic and genotypic polymorphisms of the gene involved in heat stress (HSP90β) in Marandi indigenous, broiler and laying chickens using PCR-RFLP technique. Randomly, blood was taken from 300 chicken and genomic DNA was extracted by salting out method. Amplification of the desired gene locus with a length of 494 bp was performed using specific primers and MspI enzyme was used to identify the mutation in the desired gene locus. After enzymatic digestion, two genotypes M1M1 and M1M2 and two alleles M1 (with a band of 494 bp) and M2 (with two bands of 248 bp and 246 bp) were identified for the HSP90β marker locus. Marandi indigenous and broiler chicken masses were in the Hardy-Weinberg equilibrium. For Marandi indigenous and broiler chicken populations, the Shannon information index at the HSP90β marker locus was 0.25 and 0.40, the fixation index is -0.07 and -0.16, and the observed heterozygosity index was 0.14 and 0.28, respectively. Due to the presence of polymorphism and mutation in the studied gene locus, it can be used in the Marandi indigenous and broiler chickens by genetic selection with the help of this marker to eliminate heat-sensitive chickens and keep heat-resistant chickens. | ||
| کلیدواژه‌ها [English] | ||
| Chicken, Genetics breeding, Genotype diversity, Gene marker, Heat shock | ||
| مراجع | ||
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