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تأثیر تزریق داخل صفاقی اسید آمینه تریپتوفان بر مصرف خوراک، دمای بدن و برخی متابولیت‌های بیوشیمیایی بره در طول استرس گرمایی تابستانه | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 12، شماره 3 - شماره پیاپی 47، اسفند 1402، صفحه 15-27 اصل مقاله (1.06 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2024.67071.1901 | ||
| نویسندگان | ||
| نبی الله سالاری1؛ یدالله بدخشان* 2؛ روح الله میرمحمدی2؛ جمیل بهرام پور2؛ ارسلان برازنده2 | ||
| 1سازمان جهاد کشاورزی جنوب استان کرمان، کرمان، ایران | ||
| 2گروه علوم دامی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران | ||
| چکیده | ||
| تریپتوفان سومین اسیدآمینه ضروری بدن و پیش‌ساز هورمون‌های مهمی مانند سروتونین و ملاتونین در بدن است. این اسید آمینه به دو مسیر متابولیکی کینورنین و سروتونین وارد و در یکی از این دو مسیر متابولیزه می‌شود، که تنها پنج درصد آن در مسیر سروتونین است و باقی آن در مسیر کینورنین وارد می‌گردد. به‌منظور بررسی تأثیر تزریق داخل صفاقی اسید آمینه تریپتوفان بر مصرف خوراک، دمای بدن و برخی متابولیت‌های بیوشیمیایی گوسفند 20 رأس بره (10 رأس نر و 10 رأس ماده) هشت ماه سن، وزن 5/3± 9/24 کیلوگرم و در قالب طرح فاکتوریل استفاده شد. بره‌ها به دو گروه کنترل (تزریق سرم فیزیولوژیکی به‌صورت داخل صفاقی) و گروه تیمار (تزریق‌شده با 50 میلی‌گرم تریپتوفان/ کیلوگرم وزن بدن به‌صورت داخل صفاقی) تقسیم شدند. طول دوره عادت‌دهی بره‌ها به شرایط آزمایشی هفت روز بود. پس از تزریق بلافاصله خوراک کاملاً مخلوط به نسبت 40 درصد کنسانتره و 60 درصد علوفه در اختیار بره‌ها قرار گرفت و در فواصل دو ساعت و به‌مدت چهار ساعت میزان مصرف خوراک اندازه‌گیری شد. میزان دمای رکتوم، ضربان قلب و تعداد تنفس در هر ساعت به‌مدت چهار ساعت انجام شد. این آزمایش نشان داد که خوراک مصرفی تجمعی در تیمار تریپتوفان نسبت به گروه کنترل کاهش یافت، هم‌چنین بره‌های ماده به‌طور معنی‌داری مصرف خوراک تجمعی کم‌تری داشتند (05/0P<). هم‌چنین تریپتوفان به‌روش تزریق صفاقی سبب افت مصرف خوراک تجمعی در دو ساعت اول آزمایش در مقایسه با گروه شاهد شد، اما در ادامه این کاهش جبران شد. تعداد ضربان قلب در جنس نر و تیمار تریپتوفان کاهش یافت (05/0P<). تزریق تریپتوفان به‌طور معنی‌داری باعث کاهش دمای رکتومی شد (05/0P<). تزریق تریپتوفان تأثیر معنی‌داری بر غلظت هورمون‌های T3 و T4 سرم بره‌ها نشان نداد (05/0P>)، اما نسبت هورمون T3 به T4 را کاهش داد (05/0P<). یعنی تبدیل هورمون تیروکسین به تری‌یدو تیرونین در گروه دریافت‌کننده تریپتوفان افزایش یافت. سطح کورتیزول، کلسترول، تری‌گلیسیرید، کراتین و اوره در بره‌های دریافت‌کننده تریپتوفان نسبت به گروه کنترل تفاوت معنی‌دار مشاهده نشد (05/0P>). غلظت آلبومین سرم در بره‌های دریافت‌کننده تریپتوفان نسبت به گروه کنترل کاهش یافت (05/0P<). با توجه به نتایج به‌دست آمده از این آزمایش و کاهش ضربان قلب و دمای رکتومی در تیمار تریپتوفان می‌توان نتیجه گرفت که تریپتوفان با تأثیر بر مکانیسم‌های مقابله با افزایش دمای بدن باعث بهبود وضعیت دام در شرایط تنش گرمایی شد. | ||
| کلیدواژه‌ها | ||
| تزریق درون صفاقی؛ تریپتوفان؛ تنش گرمایی؛ متابولیت‌های خونی؛ گوسفند | ||
| عنوان مقاله [English] | ||
| Effect of intraperitoneal injection of the L-tryptophan on feed intake, rectal temperature, and serum biochemical metabolites of Kermani lamb | ||
| نویسندگان [English] | ||
| Nabiallah Salari1؛ Yadollah Badakhshan2؛ Rohallah Mirmahmoudi2؛ Jamil Bahrampour2؛ Arsalan Barazandeh2 | ||
| 1Jahad-Keshavarzi organization in south of Kerman Province, Kerman, Iran | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran | ||
| چکیده [English] | ||
| Tryptophan is 3 th essential amino acid for the body. Also an important substrate for essential hormones such as serotonin and melatonin. This amino acid is metabolized in two ways in the body 1- Kynurunine pathway and 2- serotonin pathway. Tryptophan entry to These metabolizing pathways is five percent for serotonin production and ninety and five percent for kynurenine production. This experiment was performed to evaluate the Effect of intraperitoneal injection of the L-tryptophan on feed intake, rectal temperature, and some blood biochemical metabolites of the Kermani sheep breed. 20 eight-month-old lambs grouped in 10 males and 10 females in a factorial design. Lambs were divided into control groups (saline intraperitoneal injection) and treatment groups (intraperitoneal injection with 50 mg tryptophan amino acid/kg body weight ). The Control group of each gender was injected only with physiological serum intraperitoneally .immediately after injection, the feed was offered to them and cumulative feed intake was measured in 2 hours intervals for a four-hour duration. Feed was a total mixed ration consisting of 40 % concentrated and 60 % roughage. Heartbeat, respiration rate, and rectal temperature were measured every hour after amino acid or saline injection. This experiment showed that feed intake in tryptophan treatment was reduced compared to the control group, also female lambs had significantly lower cumulative feed intake (P<0.05). Heart rate decreased in males and tryptophan treatment (P<0.05). Tryptophan injection significantly reduced rectal temperature (P<0.05). Tryptophan injection did not show a significant effect on the serum concentrations of T3 and T4 hormones of lambs (P>0.05) but reduced the ratio of T3 to T4 (P<0.05). this means thyroxin converted to triiodothyronine more effectively in the group that received tryptophan amino acid. Levels of cortisol, total protein, cholesterol, triglyceride, creatine, and urea were not significantly different in the tryptophan-receiving lamb compared to the control group (P>0.05). Serum albumin concentration decreased in tryptophan-receiving lambs compared to the control group (P<0.05).blood Albumin level was decreased in tryptophan-injected lamb compared with the control group (P<0.05). According to the results of this experiment, reduction of heart rate and rectal temperature in tryptophan treatment, it could be concluded that tryptophan improves the condition of livestock under heat stress by affecting the mechanisms of coping with high ambient temperature. These mechanisms are related to heartbeat and respiration rate control centers that were affected by intraperitoneal injection of tryptophan amino acid. Tryptophan in route of intraperitoneal injection significantly reduced cumulative feed intake but this was compensated for some hours of post-injection. | ||
| کلیدواژه‌ها [English] | ||
| Blood Parameter, Heat stress, intraperitoneal injection, Kermani sheep, L-tryptophan | ||
| مراجع | ||
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