Proteome analysis of root in wheat (Triticum aestivum cv. Baran) under water deficit stress | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| Article 3, Volume 14, Issue 2 - Serial Number 48, January 2025, Pages 35-49 PDF (662.26 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.30473/cb.2024.71391.1970 | ||
| Authors | ||
| Sayna Toraby* 1; Mahmoud toorchi2; Mozaffar Roostaei3 | ||
| 1Independent | ||
| 2Prof. of the Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran | ||
| 3Dryland Agricultural Research Institute (DARI) | ||
| Abstract | ||
| Cereals, especially wheat, is one of the most important food sources for humans. Approximately 55% of proteins, 15% of fats, 70% of glucosides, and generally 50-55% of calories consumed by human come from cereals. Water scarcity is an important limiting factor in wheat production in rainfed and irrigated farming can affect different traits. Therefore, adaptation of plants to drought stress is critical to increase production. Many factors such as genotype, growth stage, intensity and duration of stress, physiological growth stage, different patterns of gene expression and environmental factors can influence the response of plants to drought stress. Different methods have been used to study the expression of genes, and proteomic analysis has priority for the study of the final gene product. In this regard, the seeds of wheat cultivars were gown in plastic pots in a greenhouse and divided into two and root random groups one month after growing in which water deficit stress was imposed to half of the pot, randomly, by increment of irrigation internal. Twenty days after imposing stress, shoot and root length, shoot dry and wet weight, root volume and proline content were measured in both groups. Protein extraction was performed by phosphate buffer method and two-dimensional electrophoresis was performed by IEF in 1st dimension and SDS-PAGE in 2nd dimension. Proteomic analysis of root tissue by two-dimensional electrophoresis with Coomassie brilliant blue staining revealed 99 repeatable protein spots each gel. Among the identified protein spots, 15 spots were shown a significant change in expression under water deficit stress condition compared with the control, in such a way 13 spots increased expression and 2 spots with reduced expression. These spots were identified according to the isoelectric point and the molecular weight. These proteins based on functional groups were classified in starch synthesis, light respiration and metabolism, proteins involved in cell structure, stress response and defense, and various proteins. Proteins Peroxidase, Phosphoglycerate mutase, Triose phosphate-isomerase, Adenosine diphosphate glucose pyrophosphorylase, Glutathione S-transferase, Monomeric alpha-amylase inhibitor, Cytosolic-3-phosphoglycerate kinase, Probable voltage-gated potassium channel subunit beta, Serine hydroxy methyltransferase, late embryogenesis abundant protein D-29, β-Hydroxyisobutyryl-CoA hydrolase, 2-Cys peroxiredoxin BAS1 and other late embryogenesis abundant proteins were identified with increased changed expression under water deficit stress conditions indicating the importance of these proteins in reducing the effects of water deficit stress. The proteins S-Adenosylmethionine synthetase 3 and Germin-Like protein were shown reduced changed expression. The result of this research indicated that different groups of proteins interfering in reduction of destructive effects of water deficiency stress but the contribution of response / defense and metabolism proteins were more than the others. | ||
| Keywords | ||
| Iso-electric focusing (IEF); Mass spectrometry; SDS-PAGE; Two-dimensional electrophoresis | ||
| Main Subjects | ||
| Proteomics | ||
| References | ||
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