Molecular isolation of Lactobacillus acidophilus and investigation of blood parameters caused by diazenone poisoning in Hypophthalmichthys nobilis | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| Volume 13, Issue 2 - Serial Number 50, March 2025, Pages 1-9 PDF (956.96 K) | ||
| Document Type: Article | ||
| DOI: 10.30473/eab.2025.72947.1973 | ||
| Authors | ||
| Azam Ghorbannia Delavar* 1; Saeed Alinejad Moallem* 2 | ||
| 1Department of Biology, Payam Noor University, Tehran, Iran | ||
| 2Department of Laboratory Sciences, Islamic Azad University, Babol Branch, Iran | ||
| Abstract | ||
| Diazenone is one of the most important organospheric pesticides that is widely used in agriculture and homes to control insects in the soil, plants and other crops. Diazenone is used to control plant pests in many agricultural fields that are located near fresh water sources, It is common.Therefore, in this research, the effect of agricultural poison diazenon was investigated by calculating LC50 on blood parameters and lactobacilli in the intestine of Hypophthalmichthys nobilis. In this experiment, fish were exposed to different concentrations of diazenon poison for 15 days. The amount of blood parameters of red and white blood cells and the presence of lactobacillus species were investigated in 6 treatments with 3 repetitions over a period of 15 days. Then, blood sampling and sampling from the intestines of Hypophthalmichthys nobilis were done. The results showed that the hematological indices of Red carp exposed to different doses of diazenon poison showed a significant decrease in RBC and WBC with increasing poison concentration (p<0.05). Also, the results showed that in all different doses of diazenon poison, the lack of growth of Lactobacillus bacteria compared to the control group on MRS agar culture medium had significant differences (p<0.05). Finally, it can be mentioned that the use of intestinal and blood Lactobacillus samples to investigate the microbial and hematological indicators of Sargandeh carp can be used as biomarkers for measuring and tracking the effects of diazenon poison in aquatic communities. | ||
| Keywords | ||
| Diazenon; Hypophthalmichthys nobilis; blood parameters; lactobacillus | ||
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