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شناسایی، پیش‌بینی عملکرد و اعتبارسنجی LncRNAs کلیدی درگیر در غده‌زایی سیب‌زمینی تحت تنش کمبود ازت | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| دوره 14، شماره 4 - شماره پیاپی 50، شهریور 1404، صفحه 43-56 اصل مقاله (1.51 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2025.72513.1985 | ||
| نویسندگان | ||
| امین اله چلوی1؛ فرهاد نظریان فیروزآبادی* 2؛ سید سجاد سهرابی3؛ ابوذر سورنی4 | ||
| 1گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان | ||
| 2گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آباد، ایران | ||
| 3گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| 4گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان، ایران | ||
| چکیده | ||
| نیتروژن (N) یکی از مهمترین مواد غذایی برای رشد و نمو سیب‌زمینی (Solanum tuberosum L.) است. در جریان تکامل، تغییرات ژنتیکی حیاتی به‌وسیله مولکول‌های RNAهای بلند غیرکدکننده ( LncRNA Lon non coding RNAs:) در پاسخ گیاهان به تنش‌های غیرزیستی به‌وجود آمده است. با توجه به اینکه اطلاعات اندکی از مکانیسم عمل و نقش این عناصر مهم تنظیمی در مرحله نمو غده سیب‌زمینی در دسترس است؛ پژوهش حاضر با هدف شناسایی، بررسی بیان و کارکرد lncRNAها و همچنین ژن‌های هدف آن‌ها در پاسخ به تیمار ازت انجام شد. نتایج این مطالعه نشان داد که در مجموع تعداد 211 مولکول lncRNA با بیان متفاوت تحت تیمار ازت شناسایی شدند. از این تعداد 4 توالی lncRNA بین‌ژنی تحت تیمار ازت بیان متفاوت داشتند که ژن‌های هدف آن‌ها با رونوشت ژن‌های مرتبط با هموستازی یونی، انتقال پیام و تخریب پروتئین همولوژی داشتند. مسیرهای متابولیکی با بیشترین فراوانی ژن‌های هدف شامل بیوسنتز متابولیت‌های ثانویه، متابولیسم اسیدهای چرب و تخریب اسیدهای چرب بودند. این مطالعه برای اولین‌بار توالی lncRNA را در سیب‌زمینی تحت تیمار ازت شناسایی نمود و به نظر می‌رسد گامی مفید در شناخت عملکرد lncRNA در چگونگی پاسخ گیاهان به تیمار ازت باشد. مکان‌یابی و عملکرد این lncRNA و فعالیت ژن‌هایی با بیان افتراقی، سبب درک بهتر مکانیسم‌های پاسخ به تیمار ازت می‌شود. | ||
| کلیدواژه‌ها | ||
| ژن‌های مرتبط با تنش؛ RNA غیرکدکننده؛ بیان ژن | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| Identification, functional prediction, and verification of key lncRNAs involved in potato (Solanum tuberosum L.) tuberization under nitrogen deficiency stress | ||
| نویسندگان [English] | ||
| Aminollah Chalavi1؛ Farhad Nazarian-Firouzabadi2؛ Seyed Sajad Sohrabi3؛ Aboozar Soorni4 | ||
| 1Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University | ||
| 2Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
| 3Production Engineering and Plant Genetics Department, Faculty of Agriculture, Lorestan University, Khorramabad, Iran | ||
| 4Department of Agricultural Biotechnology, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| چکیده [English] | ||
| Nitrogen (N) is one of the most important nutrients needed for the growth and development of potato (Solanum tuberosum L.). In the course of evolution, long non-coding RNA molecules (lncRNAs) have played a role in causing significant changes in plant’s responses to abiotic stresses. Given the limited information available on the mechanisms of action and the role of such important regulatory elements during potato tuber developmental stages, this study was conducted to identify and investigate the expression and function of lncRNAs, as well as their target genes, in response to nitrogen treatment. The results of the present study revealed that a total of 211 differentially expressed lncRNA molecules were detected under nitrogen treatment. Of these, four intergenic lncRNA sequences were differentially expressed under nitrogen treatment, and their target genes were associated with ion homeostasis, signal transduction, and protein degradation. The metabolic pathways most frequently associated with the target genes involved the biosynthesis of secondary metabolites, fatty acid metabolism, and fatty acid degradation. This study identified lncRNAs in potatoes under nitrogen treatment for the first time, marking a significant step towards understanding the function of lncRNA in plant’s responses to nitrogen. Identifying the location and function of these lncRNAs, along with the activity of differentially expressed genes, will provide valuable insights into the mechanisms underlying the plant's response to nitrogen. | ||
| کلیدواژه‌ها [English] | ||
| Stress-related genes, non-coding RNA, Gene expression | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 69 تعداد دریافت فایل اصل مقاله: 60 |
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