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توالییابی و آنالیز ژنوم رقم جهش یافته متحمل به تنش خشکی جهت شناسایی ژنهای کاندید تحمل به خشکی در برنج | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 5، دوره 15، شماره 1 - شماره پیاپی 51، آذر 1404، صفحه 63-78 اصل مقاله (1.49 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2025.73217.1996 | ||
| نویسندگان | ||
| بنفشه فتاح1؛ عبدالرضا باقری* 2؛ علی اکبر عبادی3؛ علیرضا سیفی4 | ||
| 1گروه بیوتکنولوژی و به نژادی گیاهی دانشگاه فردوسی مشهد | ||
| 2 استاد، گروه بیوتکنولوژی و بهنژادی گیاهی، دانشکده کشاورزی دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| 3دانشیار، مؤسسه تحقیقات برنج کشور، سازمان تحقیقات، آموزش و ترویج کشاوزی، رشت، ایران. | ||
| 4دانشیار، گروه بیوتکنولوژی و بهنژادی گیاهی، دانشکده کشاورزی دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| چکیده | ||
| برنج یکی از محبوبترین محصولات غذایی جهان است و تنش خشکی به عنوان مهمترین چالش تولید آن، ضرورت توسعه ارقام متحمل به تنش خشکی را ایجاب میکند. تکنیکهای توالییابی نسل جدید از طریق شناسایی پلیمورفیسم در DNA، حتی در ارقام بسیار نزدیک، فرصتهایی را برای درک اساس ژنتیکی تفاوتهای فنوتیپی مانند پاسخ به تنشهای غیرزیستی فراهم میکنند. شناسایی SNPها و InDelها یکی از مهمترین کاربردهای توالییابی نسل جدید است. این تحقیق با هدف شناسایی ژنهای کاندید تحمل به خشکی در لاین موتانت برنج متحمل به تنش خشکی با توالییابی مجدد ژنوم برای این لاین و رقم مادری آن (هاشمی، که به خشکی حساس است)، انجام شد. در ژنوم این دو رقم تعداد 73,077 پلیمورفیسم شامل پلیمورفیسم تک نوکلئوتیدی (SNPs) و InDels شناسایی شد. تفرق واریانتهای بزرگ اثر در جمعیت F2 حاصل از تلاقی این دو رقم در لاینهای حساس و متحمل به خشکی، بررسی شد. SNPهای شناسایی شده با مواردی که در مطالعات قبلی گزارش شده بودند اعتبار سنجی شدند. علاوه بر این، ارتباط عملکردی SNPها بر اساس حضور آنها در مناطق QTL و دومینهای عملکردی حفاظت شده تجزیه و تحلیل شدند. این مطالعه یک ژن هدف امیدوارکننده برای تحمل به تنش خشکی معرفی میکند که میتواند منبع ارزشمندی در برنامههای بهنژادی مولکولی برای تحمل به تنش خشکی برنج باشد. | ||
| کلیدواژهها | ||
| تحمل به خشکی؛ ) SNPپلیمورفیسم تک نوکلئوتیدی(؛ InDel )درج/حذف( | ||
| موضوعات | ||
| بیوتکنولوژی و تنش های زنده و غیرزنده | ||
| عنوان مقاله [English] | ||
| A genome sequencing approach to identify candidate genes for drought tolerance in rice, using a drought-tolerant mutant | ||
| نویسندگان [English] | ||
| Banafsheh Fattah1؛ Abdolreza Bagheri2؛ AliAkbar Ebadi3؛ Alireza Seifi4 | ||
| 1Department of Biotechnology and Plant Breeding Faculty of Agriculture, Ferdowsi University of Mashhad | ||
| 2Professor, Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 3Associate Professor, Rice Research Institute of Iran (RRII), Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran. | ||
| 4Associate Professor, Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| چکیده [English] | ||
| Rice is a globally important food crop, and drought is a major constraint on its production, highlighting the need for drought-tolerant varieties. Next-generation sequencing (NGS) enables the identification of DNA variations, even within closely related rice lines, offering a powerful approach to unravel the genetic basis of traits like drought tolerance. Identifying single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) is a crucial application of NGS. This study aimed to identify candidate genes for drought tolerance by re-sequencing the genome of a drought-tolerant rice mutant and comparing it to its drought-sensitive parent, Hashemi. We identified 73,077 polymorphisms (SNPs and InDels) between the two lines. We then analyzed the segregation of large-effect variants in a segregating F2 population derived from a cross between the two lines, comparing drought-sensitive and drought-tolerant progeny. The identified SNPs were validated against previously reported SNPs, and their functional relevance was assessed based on their location within known quantitative trait loci (QTLs) and conserved protein domains. This research pinpoints a potentially valuable target gene for enhancing drought tolerance in rice breeding programs. It is hoped that by inducing targeted mutations or using genome editing techniques to produce this protein in drought-sensitive rice varieties, cultivars can be developed that, in addition to having the desired traits, will also be tolerant to drought stress. | ||
| کلیدواژهها [English] | ||
| Drought tolerance, InDels (insertions/deletions), Rice (Oryza sativa), SNPs (single-nucleotide polymorphisms) | ||
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