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تعیین جنسیت مولکولی و بررسی تنوع ژنتیکی جوجوبا (Simmondsia chinensis Link Schneider) با نشانگرهای مولکولی ISSR و RAPD | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| دوره 15، شماره 2 - شماره پیاپی 52، دی 1404، صفحه 1-12 اصل مقاله (5.35 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2025.73765.2004 | ||
| نویسندگان | ||
| زهرا نبی قهفرخی1؛ حسین شاهسوند حسنی* 2؛ فرهاد نظریان فیروز آبادی1؛ عسکر غنی3 | ||
| 1گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران. | ||
| 2گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران. | ||
| 3گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه جهرم، جهرم، ایران. | ||
| چکیده | ||
| جوجوبا (Simmondsia chinensis Link Schneider) معروف به گیاه معجزه، گیاهی چندساله و مقاوم به تنشهای زیستی و غیرزیستی است که به دلیل تولید روغن صنعتی باکیفیت، از اهمیت اقتصادی بالایی برخوردار است. تشخیص جنسیت گیاهچههای جوجوبا در مراحل اولیه رشد، برای بهینهسازی نسبت گیاهان نر و ماده و افزایش بهرهوری در باغهای تجاری ضروری است. در این پژوهش، تنوع ژنتیکی 17 ژنوتیپ جوجوبای بذری (9 ماده و 8 نر) با استفاده از نشانگرهای RAPD و ISSR بررسی شد. هدف، اعتبارسنجی دو نشانگر اختصاصی جنسیت شامل ISSR (UBC-807 و CAPS) و دو نشانگر RAPD (F1 و F10) بود. نتایج نشان داد نشانگرهای مورد استفاده توانایی تفکیک قطعی جنسیت را از طریق تکثیر باند اختصاصی هر جنس نداشتند، اما تنوع ژنتیکی قابل توجهی بین ژنوتیپهای نر و ماده مشاهده شد. آغازگر F1 و CAPS به ترتیب بالاترین (82.5%) و پایینترین (77.8%) چندشکلی را نشان دادند. همچنین، آغازگر F10 بیشترین تنوع شانون، تعداد آلل مؤثر، شاخص باند و هتروزیگوسیتی مورد انتظار را دارا بود. دندروگرام حاصل از ضریب تشابه جاکارد توانست ژنوتیپهای نر و ماده را بهخوبی تفکیک کند. بررسیها نشان داد ISSR در مقایسه با RAPD در بررسی تنوع ژنتیکی کارآمدتر است و آغازگر F1 بهطور ویژه ژنوتیپهای نر را با دقت تفکیک کرد. این یافتهها نشان میدهد که علیرغم عدم توانایی تکثیر قطعی باندهای اختصاصی جنسیت، نشانگرهای مورد استفاده در شناسایی تنوع ژنتیکی مؤثر بوده و میتوانند با کاهش هزینههای احداث باغهای جوجوبا، الگویی برای تفکیک جنسیت در سایر گونههای زراعی، باغی و دارویی باشند. | ||
| کلیدواژهها | ||
| تشخیص مولکولی؛ چندشکلی؛ گیاه معجزه (جوجوبا)؛ نشانگرهای جنسیت؛ واکنش زنجیرهای پلیمراز | ||
| موضوعات | ||
| اصلاح نباتات مولکولی | ||
| عنوان مقاله [English] | ||
| Application of Molecular Markers for Sex Determination and Genetic Diversity Analysis of Jojoba (Simmondsia chinensis) Based on ISSR and RAPD Molecular Markers | ||
| نویسندگان [English] | ||
| Zahra Nabi Ghahfarokhi1؛ hossen Shahsavand Hassani2؛ Farhad Nazarian-Firouzabadi1؛ Askar Ghani3 | ||
| 1Production Engineering and Plant Genetic Department, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
| 2Production Engineering and Plant Genetic Department, Faculty of Agriculture, Shiraz University, Shiraz, Iran | ||
| 3horticulture science department, Faculty of Agriculture, Jahrom University, Jahrom, Iran. | ||
| چکیده [English] | ||
| Jojoba (Simmondsia chinensis Link Schneider), often referred to as the "miracle plant," is a dioecious, cross-pollinated, evergreen, and diploid (2n = 52) perennial shrub. Renowned for its significant economic value, jojoba is widely utilized in the pharmaceutical, health, and even in military industries. Moreover, jojoba remarkable resistance to both biotic and abiotic stresses enhances its environmental adaptations. Determining the sex of jojoba plants at the seedling stage (1–3 years old) is crucial for optimizing the male-to-female plant ratio for establishment and for commercial cultivation. This optimization facilitates increased productivity and yield per unit area, ensuring efficient garden establishment and management. This study assessed the genetic diversity of 17 jojoba (Simmondsia chinensis) trees, derived from the Arizona cultivar, consisting of 9 female and 8 male genotypes. These genotypes were selected based on prior research conducted at the only jojoba germplasm garden in Iran, located in Fars Province. The study employed RAPD and ISSR molecular markers to determine the sex of the jojoba genotypes and to evaluate their genetic diversity. The primary objective was to validate four specific sex determination markers: two ISSR markers (UBC-807 and CAPS) and two RAPD markers (F1 and F10). The results of this study revealed that although the molecular markers used were unable to definitively distinguish male and female jojoba genotype by amplify sex specific band, significant genetic diversity was observed between the sexes. Among the markers, the F1 primer exhibited the highest polymorphism (82.5%), while the CAPS primer showed the lowest polymorphism (78.77%). Furthermore, the F10 primer demonstrated the highest, and the CAPS primer the lowest, values for the number of effective alleles, Shannon diversity index, band index, and expected heterozygosity, making F10 the most effective and CAPS the least effective marker for assessing genetic diversity between male and female genotypes. The genetic diversity dendrogram, constructed using the Jaccard similarity coefficient, effectively differentiated male and female genotypes. Although the markers used in this study were unable to replicate the sex-specific bands reported in previous studies, the F1 primer demonstrated strong potential for distinguishing male and female genotypes based on genetic diversity. Additionally, ISSR markers were found to be more efficient than RAPD markers in evaluating genetic diversity both within and between genotypes. These findings provide a valuable framework for sex differentiation in other agriculturally, horticulturally, and medicinally important species, potentially reducing the costs associated with maintaining and establishing relevant orchards. | ||
| کلیدواژهها [English] | ||
| Miracle plant (jojoba), molecular detection, polymerase chain reaction, polymorphism, sex markers | ||
| مراجع | ||
|
Agarwal, M., Shrivastava, N., & Padh, H. (2011). Development of sex‐linked AFLP markers in Simmondsia chinensis. Plant Breeding, 130(1), 114-116. https://doi.org/10.1111/j.1439-0523.2009.01749.x
Agrawal, V., Sharma, K., Gupta, S., Kumar, R., & Prasad, M. (2007). Identification of sex in Simmondsia chinensis (Jojoba) using RAPD markers. Plant Biotechnology Reports, 1, 207-210. https://doi.org/10.1007/s11816-007-0031-6
Al-Dossary, O., Alsubaie, B., Kharabian-Masouleh, A., Al-Mssallem, I., Furtado, A., & Henry, R. J. (2021). The jojoba genome reveals wide divergence of the sex chromosomes in a dioecious plant. Plant J, 108(5), 1283-1294. https://doi.org/10.1111/tpj.15509
Al-Obaidi, J. R., Suliman, B. K., & Al-Ani, N. K. (2012). Molecular-based marker for sex differentiation of jojoba in vivo and in vitro Iraqi cultivars using RAPD-PCR. Scientific Research and Essays, 7(4), 522-527. https://doi: 10.5897/SRE11.1012
Amarger, V., & Mercier, L. (1995). Molecular analysis of RAPD DNA based markers: their potential use for the detection of genetic variability in jojoba (Simmondsia chinensis L. Schneider). Biochimie, 77(12), 931-936. https://doi.org/10.1016/0300-9084(95)80004-2
Antonova, T., Guchetl, S., Tchelustnikova, T., & Ramasanova, S. (2006). Development of marker system for identification and certification of Sunflower lines and hybrids on the basis of SSR-analysis/desarrollo de sistemas de marcadores para la identificacion y cerificacion de lineas e hibridos de girasol sobre el analisis de los fragmentos SSR/development d'un systeme de marqueurs pour l'identification et la certification de lignees et d'hybrides de tournesol a' partir de l'analyse des fragments SSR'. Helia, 29(45), 63-72. https://doi.org/10.2298/hel0645063a
Benzioni, A., & Vaknin, Y. (2002). Effect of female and male genotypes and environment on wax composition in jojoba. Journal of the American Oil Chemists' Society, 79(3), 297. https://doi.org/10.1007/s11746-002-0477-7
Bhardwaj, M., Uppal, S., Jain, S., Kharb, P., Dhillon, R., & Jain, R. K. (2010). Comparative assessment of ISSR and RAPD marker assays for genetic diversity analysis in jojoba [Simmondsia chinensis (Link) Schneider]. Journal of Plant Biochemistry and Biotechnology, 19, 255-258. https://doi.org/10.1007/BF03263351
Diniz-Filho, J. A. F., Soares, T. N., Lima, J. S., Dobrovolski, R., Landeiro, V. L., Telles, M. P. d. C.,…Bini, L. M. (2013). Mantel test in population genetics. Genetics and molecular biology, 36, 475-485. https://doi.org/10.1590/S1415-47572013000400002
Giuliano, P., Spilimbergo, A., & Tonon, G. (2006). Genetic, cultural and geographical distances, 2229, 1-30 http://ssrn.com/abstract=921405
Hosseini, F., Shahsavand Hassani, H., Arvin, M., Baghizadeh, A., & Mohammadi-Nejad, G. (2011). Sex determination of jojoba (Simmondsia chinensis cv. Arizona) by random amplified polymorphic DNA (RAPD) molecular markers. 1684-5315
Ince, A. G., Karaca, M., & Onus, A. N. (2010). A reliable gender diagnostic PCR assay for jojoba (Simmondsia chinensis (Link) Schneider). Genetic resources and crop evolution, 57, 773-779. https://doi.org/10.1007/s10722-009-9516-1
Kim, B.-R., Shin, J., Guevarra, R. B., Lee, J. H., Kim, D. W., Seol, K.-H.,…Isaacson, R. E. (2017). Deciphering diversity indices for a better understanding of microbial communities. Journal of microbiology and biotechnology, 27(12), 2089-2093. https://doi.org/10.4014/jmb.1709.09027
Kumar, J., Heikrujam, M., Sharma, K., & Agrawal, V. (2019). SRAP and SSR marker-assisted genetic diversity, population structure analysis and sex identification in Jojoba (Simmondsia chinensis). Industrial crops and products, 133, 118-132. https://doi.org/https://doi.org/10.1016/j.indcrop.2019.03.006
Liu, X., Zhang, L., & Yang, S. (2022). Analysis of Floral Organ Development and Sex Determination in Schisandra chinensis by Scanning Electron Microscopy and RNA-Sequencing. Life (Basel), 12(8). https://doi.org/10.3390/life12081260
Liu, Y., Liu, M.-Y., Qi, Y.-X., Jin, X.-Y., Xu, H.-R., Chen, Y.-x.,…Su, H.-p. (2022). Synthesis and properties of wax based on waste cooking oil. RSC advances, 12(6), 3365-3371.
Milbourne, D., Meyer, R., Bradshaw, J. E., Baird, E., Bonar, N., Provan, J.,…Waugh, R. (1997). Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Molecular breeding, 3, 127-136. https://doi.org/10.1023/A:1009633005390
Nahla, A., Adawy, S., Eliraq, M., El-Khasab, A., El-Aziz, Y., El-Bassel, E.,…El-Ashry, H. (2018). Selection and agronomical evaluation for some elite genotypes of jojoba. Journal of Applied Life Sciences International, 18(1), 1-12. https://doi: 10.9734/JALSI/2018/42332
Nolan, K. A., & Callahan, J. E. (2006). Beachcomber biology: The Shannon-Weiner species diversity index. Proc. workshop able, 334-338.
Palzkill, D. A., Younes, M. H., & Hogan, L. (1989). AT-1310, AT-1487, and AT-3365: clonal jojoba germplasm selected for horticultural use. 24(3), 526-527 ref. 6
Peakall, R., & Smouse, P. E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular ecology notes, 6(1), 288-295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Prevost, A., & Wilkinson, M. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and applied genetics, 98, 107-112. https://doi.org/10.1007/s001220051046
Rohlf, F. J. (1990). NTSYS-pc. Numerical taxonomy and multivariate analysis system.
Sabreena, Nazir, M., Mahajan, R., Hashim, M. J., Iqbal, J., Alyemeni, M. N.,…Zargar, S. M. (2021). Deciphering allelic variability and population structure in buckwheat: An analogy between the efficiency of ISSR and SSR markers. Saudi J Biol Sci, 28(11), 6050-6056. https://doi.org/10.1016/j.sjbs.2021.07.061
Sant’Anna, I. d. C., Silva, G. N., Carneiro, V. Q., Pontes, D. S., Nascimento, M., & Cruz, C. D. (2020). Comparison of projection of distance techniques for genetic diversity studies. Acta Scientiarum. Agronomy, 42, e42483. https://doi.org/10.4025/actasciagron.v42i1.42483
Sharma, K., Agrawal, V., Gupta, S., Kumar, R., & Prasad, M. (2008). ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotechnology Reports, 2, 239-243. https://doi.org/10.1007/s11816-008-0070-7
Solliman, M. E., Elbarbary, H. S., Abdullah, M. B., Kapiel, T. Y. S., Aboul-Soud, M. A. M., & Mohasseb, H. A. A. (2023). Discovery of the human homolog of sex-determining region (SRY) gene in dioecious plants. Saudi J Biol Sci, 30(2), 103548. https://doi.org/10.1016/j.sjbs.2022.103548
Stefan Van Dongen, T., & Winnepenninckx, B. (1996). Multiple UPGMA and neighbor-joining trees and the performance of some computer packages. Mol. Biol. Evol, 13(2), 309-313.
Sturtevant, D., Lu, S., Zhou, Z. W., Shen, Y., Wang, S., Song, J. M., … Guo, L. (2020). The genome of jojoba (Simmondsia chinensis): A taxonomically isolated species that directs wax ester accumulation in its seeds. Sci Adv, 6(11), eaay3240. https://doi.org/10.1126/sciadv.aay3240
Tomkowiak, A., Bocianowski, J., Kwiatek, M., & Kowalczewski, P. Ł. (2020). Dependence of the heterosis effect on genetic distance, determined using various molecular markers. Open Life Sciences, 15(1), 1-11. https://doi.org/10.1515/biol-2020-0001
Varshney, R. K., Chabane, K., Hendre, P. S., Aggarwal, R. K., & Graner, A. (2007). Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant science, 173(6), 638-649. https://doi.org/10.1016/j.plantsci.2007.08.010
Venkatesan, J., Ramu, V., Sethuraman, T., Sivagnanam, C., & Doss, G. (2021). Molecular marker for characterization of traditional and hybrid derivatives of Eleusine coracana (L.) using ISSR marker. Journal of Genetic Engineering and Biotechnology, 19(1), 178. https://doi.org/10.1186/s43141-021-00277-1
Verma, K. S., Ul Haq, S., Kachhwaha, S., & Kothari, S. L. (2017). RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech, 7(5), 288. https://doi.org/10.1007/s13205-017-0918-z
Wang, L.-L., Zhang, F.-Y., Dong, W.-W., Wang, C.-L., Liang, X.-Y., Suo, L.-L.,…Jiang, P.-H. (2020). A novel approach for the forensic diagnosis of drowning by microbiological analysis with next-generation sequencing and unweighted UniFrac-based PCoA. International journal of legal medicine, 134, 2149-2159. https://doi.org/10.1007/s00414-020-02358-1
Zhurakovskyi, B., Averichev, I., & Shakhmatov, I. (2023). Using the Latest Methods of Cluster Analysis to Identify Similar Profiles in Leading Social Networks. 116-126.
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آمار تعداد مشاهده مقاله: 7 تعداد دریافت فایل اصل مقاله: 6 |
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