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Green synthesized Copper Oxide Nanoparticles as Potential Nanocarriers for Anti-cancer Agent 5-Fluorouracil | ||
| Iranian Journal of Analytical Chemistry | ||
| دوره 12، شماره 2 - شماره پیاپی 24، آذر 2025، صفحه 94-104 اصل مقاله (1.03 M) | ||
| نوع مقاله: Full research article | ||
| شناسه دیجیتال (DOI): 10.30473/ijac.2026.78642.1351 | ||
| نویسنده | ||
| Effat Esmaeili Shahri* | ||
| Department of Chemistry, Payame Noor University, P.O. Box 19395-4697, Tehran, Iran | ||
| چکیده | ||
| Copper oxide nanoparticles were prepared by a green sol-gel method employing aqueous Cressa cretica leaf extract and copper(II) nitrate trihydrate as the copper precursor. The resulting nanoparticles were subsequently used as carriers for the anticancer drug 5-fluorouracil (5-FU). Physicochemical characterization was performed by Fourier-transform infrared spectroscopy (FTIR), ultraviolet–visible (UV–Vis) spectroscopy, X-ray diffraction (XRD), field-emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (FESEM/EDX), dynamic light scattering (DLS), zeta potential measurements, and thermogravimetric/differential thermal analysis (TGA/DTA). Drug-loading efficiency was quantified by high-performance liquid chromatography (HPLC), and the influence of reaction medium pH, contact time, and nanoparticle concentration on loading performance was systematically investigated to identify optimal conditions. The synthesized CuO nanoparticles were spherical, highly pure, and possessed a cubic crystalline structure, with a mean particle size of approximately 39 nm and a zeta potential of -15.6 mV. Under optimal loading conditions (pH 5.50, 5 h contact time, 0.015 g of CuO nanoparticles), approximately 40% of 5-FU was adsorbed onto the nanoparticle surface. MTT cytotoxicity assay demonstrated concentration-dependent cell death in Huh-7 hepatocellular carcinoma cells, with an IC50 of approximately 250 mg/L, a result consistent with an acceptable biocompatibility profile. | ||
| کلیدواژهها | ||
| Green method؛ Cressa cretica؛ CuO؛ NPs؛ Drug loading؛ 5؛ fluorouracil | ||
| عنوان مقاله [English] | ||
| نانوذرات اکسید مس سنتز شده به روش سبز به عنوان نانوحاملهای بالقوه برای عامل ضد سرطان 5-فلورواوراسیل | ||
| نویسندگان [English] | ||
| عفت اسماعیلی شهری | ||
| بخش شیمی، دانشگاه پیام نور، تهران، ایران | ||
| چکیده [English] | ||
| نانوذرات اکسید مس با روش سل-ژل سبز با استفاده از عصاره آبی برگ گیاه علف مورچه و نیترات مس (II) به عنوان پیشساز مس تهیه شدند. نانوذرات حاصل متعاقباً به عنوان حامل برای داروی ضد سرطان 5-فلورواوراسیل استفاده شدند. مشخصهیابی فیزیکوشیمیایی با استفاده از طیفسنجی مادون قرمز تبدیل فوریه (FTIR)، طیفسنجی فرابنفش-مرئی (UV-Vis)، پراش پرتو ایکس (XRD)، میکروسکوپ الکترونی روبشی گسیل میدانی همراه با طیفسنجی پراش انرژی پرتو ایکس (FESEM/EDX)، پراکندگی نور دینامیکی (DLS)، اندازهگیری پتانسیل زتا و آنالیز حرارتی-وزنسنجی/ تفاضلی (TGA/DTA) انجام شد. راندمان بارگذاری دارو با کروماتوگرافی مایع با کارایی بالا (HPLC) تعیین شد و تأثیر pH محیط واکنش، زمان تماس و غلظت نانوذرات بر عملکرد بارگذاری به طور سیستماتیک برای شناسایی شرایط بهینه بررسی شد. نانوذرات اکسید مس سنتز شده کروی، بسیار خالص و دارای ساختار کریستالی مکعبی با اندازه متوسط ذرات تقریباً 39 نانومتر و پتانسیل زتا 15.6- میلیولت بودند. تحت شرایط بارگذاری بهینه ( pH 5.50، زمان تماس 5 ساعت، 015/0 گرم نانوذرات اکسید مس)، تقریباً 40% از داروی 5-فلورواوراسیل روی سطح نانوذرات بارگزاری شد. سنجش سمیت سلولی MTT، مرگ سلولی وابسته به غلظت را در سلولهای کارسینوم هپاتوسلولار Huh-7 با IC50 تقریباً 250 میلیگرم در لیتر نشان داد، نتیجهای که با مشخصات زیستسازگاری قابل قبول است. | ||
| کلیدواژهها [English] | ||
| روش سبز, علف مورچه, نانوذرات اکسید مس, بارگذاری دارو, 5-فلورواوراسیل | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 1 تعداد دریافت فایل اصل مقاله: 2 |
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