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تأثیر عصاره هیدروالکلی یونجه زرد (Melilotus officinalis) بر آنزیمهای کبدی در موش‌های صحرایی نر بالغ تحت استرس مزمن | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 14، شماره 3 - شماره پیاپی 55، اسفند 1404، صفحه 37-47 اصل مقاله (1.03 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2026.77209.2028 | ||
| نویسندگان | ||
| مدینه مردانی نیا1؛ سعید ولی پور چهارده چریک* 2؛ حامد حسین زاده1 | ||
| 1گروه زیست‌شناسی، دانشکده علوم پایه دانشگاه شهرکرد، شهرکرد، ایران | ||
| 2گروه زیست‌شناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران | ||
| چکیده | ||
| سطح آنزیمهای کبدی بهعنوان نشانگر سلامت کبد عمل کرده و در آسیب کبدی افزایش می‌یابند. این پژوهش تجربی بهمنظور بررسی اثر عصاره هیدروالکلی گیاه یونجه زرد بر آنزیمهای کبدی خون در موش‌های صحرایی نر بالغ تحت استرس مزمن، طراحی و اجرا گردید. در مطالعه حاضر از40 سر موش صحرایی نر نژاد ویستار استفاده شد که بهصورت تصادفی به گروههای کنترل، شاهدتزریق و سه گروه تجربی دریاقت‌کننده استرس مزمن و تیمارشده با دوزهای 25، 50 و 100 (mg/Kg) ازعصاره یونجه زرد تقسیم‌بندی شدند. برای ایجاد استرس مزمن بی‌حرکتی، حیوانات روزانه در دستگاه مقیدکننده به‌مدت 2 ساعت، برای 21 روز قرار گرفتند. عصاره گیاهی 30 دقیقه قبل از القای استرس مزمن، روزانه برای 21 روز بهصورت درون صفاقی به حیوانات تزریق گردید. در انتها و پس از بی‌هوشی عمیق و خون‌گیری از قلب حیوانات، نمونههای سرم خون جهت ارزیابی سطح آنزیمهای کبدی، مورد استفاده قرار گرفتند. درگروه شاهدتزریق میزان AST، ALT و ALP، نسبت به گروه کنترل افزایش معنیدار را نشان دادند (001/0p<، 01/0p<، 05/0p<). عصاره یونجه زرد در دوز 50 (mg/Kg)، آنزیمهای AST و ALP را نسبت به گروه شاهدتزریق (001/0p<، 05/0p<) و آنزیم ALT را نسبت به شاهدتزریق و کنترل کاهش معنیدار داد (001/0p<، 05/0p<،). تجویز دوزهای 25 و 100 (mg/Kg) از عصاره، میزان آنزیم ALT را نسبت به گروه شاهدتزریق کاهش معنیدار داد (001/0p<، 01/0p<). اما دو آنزیم دیگر تفاوت معنیدار را نسبت به گروه شاهدتزریق نداشتند. بنابراین عصاره یونجه زرد میتواند در دوز متوسط و در این استرس، میزان بالای آنزیمهای کبدی خون را تا حدودی کاهش دهد. | ||
| کلیدواژه‌ها | ||
| استرس مزمن؛ آنزیمهای کبدی خون؛ مدل حیوانی؛ یونجه زرد | ||
| عنوان مقاله [English] | ||
| The Influences of Hydro-Alcoholic Extract of Melilotus officinalis on Blood Liver Enzymes in Adult Male Rats Under the Chronic Stress | ||
| نویسندگان [English] | ||
| Madineh Mardaninia1؛ Saeid Valipour Chahardahcharic2؛ Hamed Hosseinzadeh1 | ||
| 1Department of Biology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran | ||
| 2Department of Biology, Iz.C., Islamic Azad University, Izeh, Iran | ||
| چکیده [English] | ||
| The liver enzymes are the liver health indicators in which their increase uncovers the liver damage. This experimental research was designed to examine the hydro-alcoholic extract' effect of M. officinalis plant on blood liver enzymes in the adult male rats, under the chronic stress. To do so, 40 male Wistar rats were randomly classified into the control, vehicle-treated, and three experimental groups that received chronic stress and treated with dosages of 25, 50 and 100 (mg/Kg) of M. officinalis extract. To induce chronic immobility stress, animals were daily placed in the restraint device for two hrs during 21 days. The plant extract was injected intraperitoneally 30 minutes before stress induction. After the deep anesthesia and blood sampling from the animals' hearts, the blood serum samples were taken to evaluate the liver enzymes' level. In the vehicle-treated group, AST, ALT, and ALP levels showed significant increases compared to the control group (p<0.05, p<0.01, p<0.001, respectively). The M. officinalis extract at 50 mg/kg significantly reduced AST and ALP levels compared to the vehicle-treated group (p<0.001, p<0.05) and significantly reduced ALT levels compared to both vehicle-treated and control groups (p<0.001, p<0.05). Administration of 25 and 100 mg/kg of the extract significantly lowered ALT levels relative to the vehicle-treated group (p<0.001, p<0.01), while no significant changes were observed in the other two enzymes.As a conclusion, the medium dose of M. officinalis extract could reduce high level of the liver blood enzymes in this stress. | ||
| کلیدواژه‌ها [English] | ||
| Animal model, Blood liver enzymes, Chronic stress, Melilotus officinalis | ||
| مراجع | ||
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