پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 45 |
| تعداد شماره‌ها | 1,219 |
| تعداد مقالات | 10,473 |
| تعداد مشاهده مقاله | 20,218,603 |
| تعداد دریافت فایل اصل مقاله | 13,907,582 |
اثرات الیسیتور نانوذرات نقره و تنش خشکی بر بیان ژن بتاکاروتن هیدروکسیلاز (bch) بر عملکرد کارتنوئید زعفران (Crocus sativus L.) | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 1، دوره 6، شماره 3 - شماره پیاپی 17، خرداد 1396، صفحه 1-13 اصل مقاله (632.96 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| بتول صابرتنها* 1؛ براتعلی فاخری2؛ نفیسه مهدی نژاد3؛ زهره علیزاده4 | ||
| 1کارشناسی‌ارشد، گروه اصلاح‌نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل | ||
| 2دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل | ||
| 3استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل | ||
| 4استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بیرجند | ||
| چکیده | ||
| این پژوهش به‌منظور بررسی تأثیر تنش خشکی و نانو ذرات نقره بر بیان ژن بتاکاروتن‌هیدروکسیلاز (bch) و تولید کارتنوئید در گیاه زعفران انجام شد. آزمایش در دو سطح آبیاری نرمال و تنش خشکی کامل بر روی نه اکوتیپ زعفران در سه سطح، شاهد (آب مقطر)، 55 و 110 پی‌پی‌ام نانو ذرات نقره پیاده شد. این بررسی به‌صورت اسپیلت پلات فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در دو تکرار در مزرعه تحقیقاتی دانشگاه بیرجند و پژوهشکده زیست‌فناوری دانشگاه زابل انجام شد. بعد از تهیه نمونه‌های برگی از تمامی تیمار‌ها، استخراج RNA، ساخت cDNAو تعیین شیب دمایی، جهت بررسی الگوی بیان ژن از واکنش Real Time PCR استفاده شد. سپس داده‌ها با نرم‌افزارهای GenEX و SAS 9.2 مورد تجزیه و تحلیل قرار گرفتند. اثرات اصلی تیمارهای اکوتیپ، نانوذره نقره و تنش خشکی و اثرات متقابل آن‌ها برای بیان ژن bch و میزان کارتنوئید در سطح احتمال یک درصد معنی‌دار شد. حداکثر بیان ژن bch و مقدار کارتنوئید در سطح 55 پی‌پی‌ام نانوذره نقره تحت تنش خشکی در اکوتیپ قاین (به ترتیب 62/1478 و 37/21 میکروگرم بر گرم وزن‌تر) مشاهده شد. در نتیجه، تنش خشکی و نانوذره نقره تا سطح 55 پی‌پی‌ام موجب افزایش بیان ژن bch و میزان تولید کارتنوئید شده است. | ||
| کلیدواژه‌ها | ||
| زعفران؛ بتاکاروتن هیدروکسیلاز؛ نانو ذرات؛ Real time PCR | ||
| موضوعات | ||
| فیزیولوژی مولکولی | ||
| عنوان مقاله [English] | ||
| Effects of silver nanoparticles elicitor and drought stress on the expression of beta -carotene hydroxylase (bch) gene on the yield of saffron carotenoid (Crocus sativus L.) | ||
| نویسندگان [English] | ||
| Batool Sabertanha1؛ Baratali Fakheri2؛ nafise Mahdinezhad3؛ Zohre Alizade4 | ||
| 1M.Sc., Department of Plant Breeding and Biotechnology, College of Agriculture, University of Zabol, Iran. | ||
| 2Associate Professor, Department of Plant Breeding and Biotechnology, College of Agriculture, University of Zabol, Iran. | ||
| 3Assistant Professors, Department of Plant Breeding and Biotechnology, College of Agriculture, University of Birjand, Iran | ||
| 4Assistant Professors, Department of Plant Breeding and Biotechnology, College of Agriculture, University of Birjand, Iran | ||
| چکیده [English] | ||
| This study aimed to investigate the effect of drought stress and silver nanoparticles on beta-carotene hydroxylase (bch) gene expression and carotenoid production in saffron. The experiment was implemented in two levels including normal irrigation and full drought stress on nine ecotypes of saffron in three levels: control (distilled water), 55 and 110 ppm of silver nanoparticles levels. This study was conducted in split plot factorial experiment based on randomized complete block design with two replications at research field of Birjand University and biotechnology institute of Zabol University.In order to examine gene expression pattern, after taking leaf samples from all treatments, RNA extraction, cDNA synthesis and determination of temperature gradient, Real time polymerase chain reaction (Real-Time PCR) was used. Then, Data were analyzed using EX and SAS 9.2 software. The main effects of treatments with nine ecotypes of saffron, silver nanoparticle and drought stress and their interaction effects for bch gene expression and amount of carotenoid were significant at 1% probability level. The maximum bch gene expression and the amount of carotenoid was observed in 55 ppm of silver nanoparticles under drought stress in Ghaen ecotype (1478.62 & 21.37 µg.g-1, respectively). Therefore, drought stress and silver Nanoparticles up to 55ppm increased bch gene expression and carotenoid production. | ||
| کلیدواژه‌ها [English] | ||
| Saffron, Beta-carotene hydroxylase, nanoparticles, Real time PCR | ||
| مراجع | ||
|
Biemelt S, Hajirezai MR, Melzer M (1999) Sucrose synthase activity does not restrict glycolysis in roots of transgenic potato plants under hypoxic conditions. Planta 210: 41-49.
Browse J, Xin Z (2001) Temperature sensing and cold acclimation. Curr. Opin. Plant Biol. 4(3): 241-246.
Chang YS, Chen HC (2001) Variability between silver thiosulfate and 1-naphthaleneacetic acid applications in prolonging bract longevity of potted bougainvillea. Sci. Hort. 87: 217-224.
Chen Y, Li F, Wurtzel ET (2010) Isolation and characterization of the Z-ISO gene encoding a missing component of carotenoid biosynthesis in plants. Plant Physiol. 153(1): 66-79.
Crawford RMM, Andle RB (1996) Oxygen deprivation stress in a changing environment. J. Exp. Bot. 47: 145-159.
Cunningham FX, Gantt E (1998) Genes and enzymes of carotenoid biosynthesis in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 557-583.
Dubey S, Lahtinen M, Sillanpaa M (2010) Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa. Colloids and Surfaces A: Physicochemical and Engineering Aspects 364: 34-41.
Grilli-Caiola M (2004) Saffron reproduction biology. Acta Hortic. 650: 25-39.
Hariri F, Omidi M, Shafiei M, Parvane S (2009) Production and Intensification of carotenoid biosynthesis genes by genetic engineering, food and biotechnology Regional Conference.
Haghgoo R, Rezvani M B, Kameli S (2013) Effect of various amounts of nanosilver incorporation on the mechanical properties of resin modified glass-ionomer cement. Journal of Dental Medicine-Tehran University of Medical Sciences 26(3): 211-7.
Heidarvand L, Maal Amiri R (2010) What happen in plant molecular response to cold stress. Acta physiol. Plant. 32: 419-431.
Kamalizadeh M, Bihamta M R, Peyghambari SA, Hadian J (2014) Expression of Genes Involved in Rosmarinic Acid Biosynthesis Pathway in Dragonhead Affected by Nanoparticles. J. G3M. 12(1).
Kafi M (2002) Saffron production and processing technology. Institute Press Ferdowsi University of Mashhad. 276PP.
Kazemi shahandashti S S, Maal Amiri R, Zeinali H, Ramezanpoor S S (2013) Change in membrane fatty acid compositions and cold-induced responses in chickpea. Mol. Biol. Rep. 40: 893-903.
Kazemi M, Talebifar M, Abedin A, Safariyan A (2012) Saffron (acquaintances, crop management and production, chemical composition and cost) 1st Ed. Ayyz Press. 75 P.
Khodayari M, Omidi M, Shah nejat boshehri AA, Yazdani D, Naghavi MR (2015) Variation in the expression of genes involved in the biosynthesis of the plant Papaver somniferum L. Sngvyynaryn affected by Nano elicitors. J. Medicinal Plants (14) 2.
Khosroushahi A, Valizadeh M, Ghasempour M. Naghdibadi H (2005) Improved Taxol production by combination of inducing factors in suspension cell culture of Taxus baccata. Cell Biol. Int. 30: 262-269.
Kim J, DellaPenna D (2006) Defining the primary route for lutein synthesis in plants: the role of Arabidopsis carotenoid b-ring hydroxylase CYP97A3. Proc. Nati.Acad. Sci.U.S.A. 103, 3474-3479.
Kim J, Smith JJ, Tian L, Dellapenna D (2009) The evolution and function of carotenoid hydroxylases in Arabidopsis. Plant Cell Physiol. 50: 463-479.
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. J. Met. Enzymol. 148: 350-382.
Lin D, Xing B (2007) Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environ. Pollut. 150: 243-50.
Moshtaghi N, Ghahremanzadeh R, Marashi h (2010) Irrigation effects on pds and bch genes expression of the Iranian Saffron. J. Cell Mol. Res. 2(2): 61-66.
Molina RV, Valero M, Navarro Y (2005) Temperature effects on flower formation in saffron (Crocus sativus L.). Sci. Hort. 103:361–379.
Nazari M R, Habibpour Mehraban F, Maal Amiri R, Zeinali Khaneghah H (2012) Change in Antioxidant Responses against Oxidative Damage in Black Chickpea Following Cold Acclimation. Russ. J. Plant Physiol. 59: 183-189.
Patel H, Krishnamurthy R (2013) Elicitors in Plant Tissue Culture. J. Pharm. Phytochem. 2: 60-65.
Perata P, Alpi A (1993) Plant responses to anaerobiosis. Plant Sci. 93:1–17.
Raee M, Omidi M, Turabi S (2012) Effect of abiotic stimuli on tissue culture of Aloe Vera, Biotechnology Master's Thesis, Islamic Azad University, Sci. Res.
Rezvani N, Sorooshzadeh A, Farhadi N (2012) Effect of nano-silver on growth of saffron in flooding stress. World Academy of Science, Engineering and Technology 1: 6.
Rezvani N, Sorooshzadeh A (2014) Effect of nano-silver on root and bud growth of saffron in flooding stress condition. J. Agricul. Technol. Saffron. 2(1): 91-104.
Rodriguez-Concepcion M (2010) Supply of precursors for carotenoid biosynthesis in plants. Arch. Biochem. Biophys. 504: 118-122.
Rossel JB, Wilson IW, Pogson B.J (2002) Global Changes in Gene Expression in Response to High Light in Arabidopsis. Plant Physiol. 130: 1109-1120.
Rostami M, Mohammad parast M, Mohammad parast B, Golfam R (2013) The effect of different levels of salinity on leaf concentrations of saffron (Crocus sativus L.). National Conference on Agricultural Science and Technology.
SAS Institute (2014) SAS/Stat User’s Guide, Version 9.2. SAS Institute, Cary, NC.
Seif Sahandi M, Sorooshzadeh A, Rezazadeh H, Naghdiabadi HA (2011) Effect of nano silver and silver nitrate on seed yield of borage. J. Med. Plants Res. 5(2):171-175.
Strader LC, Beisner ER, Bartel B (2009) Silver ions increase auxin efflux independently of effects on ethylene response. Plant Cell. 21: 3585-3590.
Tabatabai pazhoh Z, Razavizadeh R, Rostami F (2013) The effect of nano silver pigments chlorophyll and carotenoids and flavonoids found in canola (Brassica napus) in vitro. Second National Conference Nanvaz technology theory and application.
Tian L, Magallanes-Lundback M, Musetti V, DellaPenna D (2003) Functional analysis of beta- and epsilon-ring carotenoid hydroxylases in Arabidopsis. Plant Cell Physiol. 15: 1320-1332.
Umezawa T, Fujita M, Fujita Y, Yamaguchi-Shinozaki K, Shinozaki K (2006) Engineering Drought Tolerance in Plants: Discovering and Tailoring Genes to Unlock the Future. Curr. Opin. Biotechnol. 17:113-122.
Vanaja V, Annadurai G (2012) Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. App. Nano sci. 3: 217-223.
Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci. 172: 861-875.
Vincour B, Altman A (2005) Recent Advances in Engineering Plant Tolerance to Abiotic Stress: Achivements and Limitations. Curr. Opin. Biotechnol. 16:123-132.
Visser E J W, Pierik R (2007) Inhibition of root elongation by ethylene in wetland and non-wetland plant species and the impact of longitudinal ventilation, Plant Cell Environ. 30 (1): 31-38 pp.
Yang HG, Sun CH, Qiao ShZ, Zou J, Liu G, Smith SC, Cheng, Lu GQ (2008) Anatase TiO2 single crystals with a large percentage of reactive facets. Nature 453: 638-641.
Yousefi K, Riahi-Madvar A, Baghizadeh A (2015) Investigation of the effects of Ag and Cu elicitors on flavones synthase 1 gene expression and some biochemical parameters on Cuminum cyminum L. endemic to Iran. J. Plant Res. (Ira. J. Biol.). 28(1).
Zengin F K, Munzuroglu O (2005) Effects of some heavy metaleson chlorophyll, proline and som antioxidant and chemicals in Bean (Phaseolus vulgaris L) seedlings. Acta Biol. Cracov. Ser. Bot.47(2):157–164.
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv. 23: 283-333. | ||
|
آمار تعداد مشاهده مقاله: 3,084 تعداد دریافت فایل اصل مقاله: 1,590 |
||