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مقایسه کمی تولید آمونیاک و تری‌ایندول استیک اسید در جدایه‌های باکتریایی شور، قلیا و شور و قلیاپسند خاک | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 5، دوره 6، شماره 1، شهریور 1396، صفحه 51-68 اصل مقاله (1.14 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
| امیر لکزیان* 1؛ مهرنوش اسکندری تربقان2؛ علی رضا آستارایی3؛ امیر فتوت3؛ حسین بشارتی4 | ||
| 1استاد گروه علوم خاک دانشکده کشاورزی دانشگاه فردوسی مشهد و نویسنده مسنول | ||
| 2دانشجوی دکتری علوم خاک دانشکده کشاورزی دانشگاه فردوسی مشهد | ||
| 3دانشیار، گروه علوم خاک دانشکده کشاورزی دانشگاه فردوسی مشهد | ||
| 4دانشیار، مؤسسه تحقیقات خاک وآب کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| جداسازی و شناسایی جدایه‌هایی با خصوصیت PGPR میتواند برای بهبود رشد گیاهان در مناطق شور مورد استفاده قرار گیرد. به منظور اندازه‌گیری کمی برخی خصوصیات PGPR در جدایه‌های باکتریایی شور، قلیا و شورو قلیاپسند بومی خاک های استان خراسان رضوی، از شش منطقه مختلف، جدایه‌ها جداسازی و خالص‌سازی شدند تا غلظت آمونیاک به روش نسلر اصلاح شده و تری ایندول استیک اسید به روش سالکووسکی در آنها اندازه گیری گردد. جدایه‌های قلیاپسند بیشترین تولید آمونیاک (055/0%) را در بین هر سه گروه باکتری نشان دادند که 5/9 برابر متوسط آن در جدایه‌های شوروقلیاپسند (0058/0 %) و 13 برابر متوسط آن در شورپسندها (004/0 %) بود. اکثر جدایه‌های شور، قلیا و شور و قلیاپسند به ترتیب با میانگین 0003/0، 0001/0 و 0021/0% تولیدکننده IAA بودند که مقدار تولیدی در گروه شور و قلیا حدود 6 برابر شورپسندها و 5/14 برابر قلیاپسندها بود. بررسی معادلات پیشبینی غلظت آمونیاک و IAA تولیدی بهترتیب فقط در جدایه‌های شوروقلیاپسند برای تولید آمونیاک (046/0=P) و در جدایه‌های شورپسند برای تولید IAA (015/0=P) تحت تأثیر EC و pH محیط کشت معنی‌دار شد (05/0P≤). معادلات رگرسیون چند متغیره جهت پیش بینی غلظتهای آمونیاک و IAA تولیدی جدایه‌ها تحت تأثیر EC وpH محیط در غلظتهای بالا و پایین از آمونیاک و IAA از کارایی چندانی برخوردار نبود. استفاده از روشهای نسلر و سالکووسکی پس از کمی تغییرات به دلیل سهولت اجرا و نتایج نسبتاً دقیق آن، قابل توصیه و پیشنهاد در تحقیقات بعدی میباشد. | ||
| کلیدواژه‌ها | ||
| تری ایندول استیک اسید؛ روش سالکووسکی؛ روش نسلر؛ شورپسند؛ شور و قلیاپسند؛ قلیاپسند | ||
| عنوان مقاله [English] | ||
| Quantitative comparison of ammonia and 3-indoleacetic acid production in halophilic, alkalophilic and haloalkalophilic bacterial isolates in soil | ||
| نویسندگان [English] | ||
| Amir Lakzian1؛ Mehrnoush Eskandari Torbaghan2؛ Ali Reza Astaraei3؛ Amir Fotovat3؛ Hossein Besharati4 | ||
| 1Professor, Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad | ||
| 2Professor, Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran | ||
| 3Associate Professor, Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran | ||
| 4Associate Professor, Soil and Water Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran | ||
| چکیده [English] | ||
| Isolation and identification of isolates with PGPR characteristic can be used to improve plant growth in saline areas. In order to quantitative measure of PGPR characteristic in native halophilic, alkalophilic and haloalkalophilic bacterial isolates of Khorasan Razavi soils, isolates were isolated and purified from six different areas to measure the concentration of ammonia production via corrected Nesslerization method and 3-indoleacetic acid as Salkowski method. The alkalophiles isolates showed maximum ammonia production (0.055%) among the three groups of bacteria which this amount was 9.5 times of its average in haloalkalophiles isolates (0.0058%) and 13 times of ammonia production average in halophiles (0.004%). Most of halophiles, alkalophiles and haloalkalophiles isolates were IAA producer with 0.0003, 0.0001 and 0.0021 percent respectively that the IAA amount in haloalkalophilic group was about 6 and 14.5 times of it in halophilic and alkalophilic isolates respectively. Equations to predict the concentration of ammonia and 3-indole acetic acid production was only significant in the haloalkalophilic isolates for ammonia production (P=0.046) and halophilic isolates for IAA production (P=0.015) under effect of electrical conductivity and pH in 0.05 probability level. Results represented that the multiple regression analysis for prediction of ammonia and IAA concentrations producing by isolates had not any significant performance in high and low concentrations under effect of electrical conductivity and pH. Uses of Nesslerization and Salkowski methods after some modifications show promises and are recommendable in research due to their ease of implementation and relatively accurate results. | ||
| کلیدواژه‌ها [English] | ||
| 3-Indoleacetic acid, Alkalophiles, Haloalkalophiles, Halophiles, Nesslerization method, Salkowski method | ||
| مراجع | ||
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