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اثرات تراکم ذخیره‌سازی بر برخی شاخصهای خونی و بیوشیمیایی سرم در ماهی کاراس طلایی Carassius auratus | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 8، دوره 6، شماره 4، خرداد 1397، صفحه 75-84 اصل مقاله (1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
| هاشم خندان بارانی* 1؛ محمدرضا حیدری2 | ||
| 1مربی، پژوهشکده تالاب بین‌المللی هامون، گروه پژوهشی شیلا،ت دانشگاه زابل | ||
| 2دانشجوی سابق کارشناسی ارشد، دانشکده منابع طبیعی، گروه شیلات، دانشگاه زابل | ||
| چکیده | ||
| چکیده در مطالعه حاضر اثر تراکم بر روی برخی از شاخصهای خون (گلبولهای قرمز و سفید) و بیوشیمیایی سرم (گلوکز، تریگلیسیرید، کلسترول، پروتئین کل، متابولیتها، آنزیمهای آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز، آلکالین فسفاتاز و هورمون کورتیزول) در ماهی کاراس طلایی (Carassius auratus) مورد بررسی قرار گرفت. بر این اساس ماهیان کاراس با میانگین وزنی 18±200 گرم به‌مدت 30 روز در دو تیمار با سطح تراکم کم (تیمار 1، Kg/m310) و تراکم زیاد (تیمار 2، Kg/m345) و سه تکرار به ازای هر تیمار به‌طور تصادفی در 6 مخزن قرار گرفتند. ماهیان در شرایط تراکم زیاد به‌طور معنیداری میزان بیشتری از کورتیزول، گلوکز و آلکالین فسفاتاز (به‌ترتیب ng/dl 9/0±36، mg/dl 2/5±1/83 و U/L1/8±2/93) را نسبت به ماهیان تحت تراکم کم (به ترتیب ng/dl4/0±3/23، mg/dl2/3±4/52 و U/L3 /6±2/61) نشان دادند، در حالیکه میزان تری گلیسیرید در اثر افزایش تراکم به‌طور معنیداری کاهش یافت (05/0P˂). تأثیر تراکم بر میزان پروتئین کل پلاسما، کلسترول، آنزیم آسپارتات آمینوترانسفراز، منیزیم، فسفر و کلسیم معنیدار نبود. در ارتباط با سلولهای خون نیز تعداد گلبولهای قرمز در ماهیان تیمار 2 به‌طور معنیداری بیشتر از ماهیان تیمار 1 بود (05/0P˂). تعداد گلبولهای سفید در ماهیان تیمار 2 کمتر بود ولی این تفاوت معنیدار نبود. به‌طور کلی نتایج این مطالعه نشان داد که تراکم زیاد در ماهی کاراس طلایی مانند بسیاری از ماهیان پرورشی دیگر باعث ایجاد استرس مزمن میشود و نتیجه آن تغییر میزان برخی شاخصها بهویژه کورتیزول، گلوکز، آنزیم آلکالین فسفاتاز و گلبولهای قرمز بود. | ||
| کلیدواژه‌ها | ||
| استرس محیطی؛ خون‌شناسی؛ شاخصهای بیوشیمیایی؛ کاراس طلایی | ||
| عنوان مقاله [English] | ||
| The effects of stocking density on some blood and serum biochemical indices of goldfish Carassius auratus | ||
| نویسندگان [English] | ||
| Hashem Khandan Barani1؛ Mohammad Reza Heydari2 | ||
| 1Instructor, International Hamoon Wetland Research Institute, University of Zabol, Iran | ||
| 2Former M. Sc. Student, Department of Fisheries, Faculity of Natural Resources, University of Zabol, Iran | ||
| چکیده [English] | ||
| Abstract In this study, the effect of stocking density on some hematological (Red and white blood cells) and serum biochemical parameters (Glucose, triglycerides, cholesterol, total protein, inorganic phosphorus, calcium and magnesium, enzymes alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and cortisol) of Carassius auratus was investigated. Fish with average weight 200±18 g were stocked randomly into 6 tank with triplicate in two treatments for a 30-day period and were submitted to different stocking densities: low density (T1, 10 kg/m3) and high density (T2, 45 kg/m3). At the end of the trial, Fish from each group were selected and their blood was collected and the following parameters were analyzed. Fish held at high density showed significant higher levels of cortisol, glucose and alkaline phosphatase (23±0.4 ng/dl, 52±3.2 mg/dl and 93.2±8.1U/L, respectively) compared with those held at low density (36±0.9 ng/dl, 83±5.2 mg/dl and 61.2±6.3U/L, respectively), while the levels of triglycerides significantly decreased with increasing density (PP | ||
| کلیدواژه‌ها [English] | ||
| Environmental stress, Hematology, Biochemical indices, Carassius auratus | ||
| مراجع | ||
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