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تحلیل فیلوژنتیکی خانواده ژنی NAC در سورگوم دانه‌ای و بررسی الگوی بیانی اعضای دخیل در پاسخ به تنش خشکی | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 1، دوره 7، شماره 3 (بهار 1397) - شماره پیاپی 21، خرداد 1397، صفحه 1-15 اصل مقاله (899.46 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| سپیده سنجری1؛ رضا شیرزادیان خرم آباد* 2؛ زهراسادات شبّر3؛ مریم شهبازی4 | ||
| 1دانشجوی دکتری بیوتکنولوژی گیاهی، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران | ||
| 2استادیار گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران | ||
| 3استادیار گروه زیست‌شناسی سیستم‌ها، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 4استادیار گروه فیزیولوژی مولکولی، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| سورگوم علی‌رغم تحمل قابل‌توجه به خشکی، در دوران قبل و بعد از گلدهی در صورت مواجهه با تنش خشکی، دچار کاهش عملکرد دانه‌ای می‌شود. عوامل رونویسیNAC ، نقش کلیدی در سازگاری سورگوم به خشکی ایفا می‌کنند. در این مطالعه، اطلاعات مربوط به خانواده پروتئینیNAC از پایگاه‌های اطلاعاتی جمع‌آوری شد. سپس، مدل مخفی مارکوف دمین NAC (PF02365) بر علیه پروتئین‌های سورگوم مورد جستجو قرار گرفت. در مجموع، 183 توالی پروتئینی کدشونده توسط 131 مکان ژنی شناسایی شدند. درخت فیلوژنی بر اساس دمینNAC خانواده ژنیNAC سورگوم، به‌همراه 11 توالی پروتئینی شناخته شده در سایر گیاهان، با روش نزدیک‌ترین همسایه‌ها ترسیم شد که این خانواده را به 15 زیرخانواده طبقه‌بندی نمود. 13 عضو از خانواده پروتئینی NAC سورگوم به زیرخانوادهSNAC های سایر گیاهان پیوستند که احتمالا در تحمل به تنش‌های غیرزیستی دخیل باشند. 14 نوع عنصر تنظیمی پاسخ‌دهنده به تنش‌ها و هورمون‌ها در راه‌انداز ژن‌های زیر‌گروه SNAC پیش‌بینی شد. به‌منظور بررسی الگوی بیانی نسبی ژن‌های SNAC، کشت مزرعه‌ای به‌صورت اسپلیت پلات در قالب طرح بلوک‌های کامل تصادفی اجرا شد. آبیاری در دو سطح شامل آبیاری معمولی و تنش خشکی (قطع آبیاری پس از گلدهی) و ارقام در دو سطح شامل کیمیا (متحمل) و سپیده (حساس) لحاظ گردید. با توجه به الگوی بیانی SbSNACها، انتظار می‌رود که برخی از اعضا به‌عنوان تنظیم‌کننده‌های رونویسی مثبت (سه عضو) و منفی (سه عضو) در پاسخ به تنش خشکی پس از گلدهی در سورگوم فعالیت کنند. همچنین، برخی در پیری برگ (دو عضو) و فرایند انتقال مجدد فلزات (دو عضو) نقش دارند. | ||
| کلیدواژه‌ها | ||
| NAC؛ خشکی؛ Sorghum bicolor (L.) Moench؛ درخت فیلوژنتیکی؛ الگوی بیانی ِژن | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| Phylogenetic analysis of NAC gene family in grain Sorghum and expression pattern analysis of drought responsive members | ||
| نویسندگان [English] | ||
| Sepideh Sanjari1؛ Reza Shirzadian-Khorramabad2؛ Zahra-Sadat Shobbar3؛ Maryam Shahbazi4 | ||
| 1Ph.D. Student, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. | ||
| 2Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. | ||
| 3Assistant Professor, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 4Assistant Professor, Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| Sorghum, in spite of its great tolerance to drought stress, suffers from grain yield loss due to pre and post flowering -drought stress conditions. NAC TFs play key roles in Sorghum drought adaptation. In this study, NAC protein family data was collected from databases. Then, hidden Markov model profiles of NAM domain (PF02365) was obtained from Pfam database and used to find the putative NAC members against Sorghum proteins. Totally, 183 protein sequences encoded by 131 gene loci were identified. The unrooted phylogenetic tree was constructed based on NAC domains of Sorghum and 11 known NAC domains of other plants using the Neighbor-Joining method, which classified the family into 15 subfamilies. 13 members of the NAC protein family of Sorghum joined to the SNAC subfamily of other plants, which are expected to be involved in abiotic stress tolerance. 14 different stress and hormone responsive regulatory elements were predicted in promoters of SNAC subgroupgenes. To study the expression pattern of these genes, two extreme Sorghum cultivars including Kimia and Sepideh were planted based on Split-plot Randomized Complete Block Design with three replications in the field. Irrigation was performed in two levels including normal irrigation and drought stress (water holding from anthesis). Based on the SbSNAC expression pattern, we predict that some members are involved in response to drought stress at post-flowering stage as positive (3 members) and negative transcriptional regulators (3 members). As well, some of them play role in leaf senescence (2 members) and metal remobilization processes (2 members). | ||
| کلیدواژه‌ها [English] | ||
| NAC, Drought, Sorghum bicolor (L.) Moench, Phylogenetic tree, gene expression pattern | ||
| مراجع | ||
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