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برهمکنش باکتریهای Bacillus amyloliquefaciens و Azospirillum oryzae در تحریک رشد گیاه گندم و کاهش بیماری زایی قارچ Fusarium graminearum | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 2، دوره 8، شماره 3 (بهار 1398) - شماره پیاپی 25، اردیبهشت 1398، صفحه 19-33 اصل مقاله (646.3 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2019.43198.1757 | ||
| نویسندگان | ||
| نگار باقری1؛ مسعود احمدزاده* 2؛ غلامرضا صالحی جوزانی3 | ||
| 1دانشجوی دکتری، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 2استاد، گروه گیاهپزشکی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 3استاد، بخش تحقیقات بیوتکنولوژی میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO) | ||
| چکیده | ||
| تلقیح توام ریزوباکتری‌های افزاینده رشد گیاه و عوامل کنترل زیستی آفات و بیماری‌ها، رویکردی در مدیریت سلامت و بهبود تولید و کیفیت محصولات کشاورزی می‌باشد. در این تحقیق به مطالعه اثرات متقابل دو سویه باکتریایی Bacillus amyloliquefaciens UTB96 و Azospirillum oryzae NBT506 در تحریک رشد گیاه گندم و بازدارندگی قارچ Fusarium graminearum (عامل بلایت خوشه و پوسیدگی طوقه گندم) در دو حالت کشت منفرد و هم‌کشتی پرداخته شد. نتایج نشان داد که باکتری‌ها در کشت منفرد و هم‌کشتی بخوبی از رشد قارچ بیماری‌زا به‌صورت مستقیم و با ترکیبات فرار جلوگیری کردند و این نتایج در شرایط اتاقک رشد هم مشاهده شد که هم‌کشتی دو سویه به شدت باعث افزایش شاخص‌های رشدی گیاه و کاهش خسارت و علائم بیماری نسبت به شاهد شد. بهترین تیمارهای باکتری‌ها در حالت محلول‌پاشی در سطح خاک بودند. در محلول‌پاشی سطح خاک با این عوامل میکروبی، طول ریشه 80-14 درصد، وزن تر ریشه 167-18 درصد، وزن خشک ریشه 110-4 درصد، طول ساقه 61-17 درصد، وزن تر ساقه 169-47 درصد و وزن خشک ساقه 90-4/0 درصد افزایش را نشان دادند همچنین درصد کاهش شاخص بیماری نیز 100- 62 درصد در تیمارهای مختلف برآورد شد. به‌طور کلی هم‌کشتی دو باکتری اثرات هم‌افزایی در افزایش رشد گندم و کاهش رشد قارچ بیمارگر نشان داد. | ||
| کلیدواژه‌ها | ||
| اثر هم‌افزایی؛ کنترل زیستی؛ هم‌کشتی؛ Bacillus amyloliquefaciens و Azospirillum oryzae | ||
| موضوعات | ||
| ایمنی زیستی | ||
| عنوان مقاله [English] | ||
| Interaction of Bacillus amyloliquefaciens and Azospirillum oryzae on wheat growth promotion and Fusarium graminearum disease inhibition | ||
| نویسندگان [English] | ||
| Negar Bagheri1؛ Masoud Ahmadzadeh2؛ Gholamreza Salehi Jouzani3 | ||
| 1Ph.D. Candidate, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Professor of Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Professor of Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| Co-inoculation of plant growth promoting bacteria and biological control agents, is a strategy to improve health, yield and quality of crop production. The objective of the present study was to evaluate interactions of two bacterial strains, Bacillus amyloliquefaciens UTB96 and Azospirillum oryzae NBT506, on growth promotion of wheat and control of the causal agent of Fusarium head blight, Fusarium graminearum. The results showed that single and co-culture of these strains inhibit mycelium growth of F. graminearum by direct inhibition and volatile organic compounds. In germinator assays, different wheat growth features were increased and disease index was decreased. Results showed that co-inoculation or single application of the bacteria in the soil significantly enhanced root length (14-80%), root fresh weight (18-167%), root dry weight (4-110%,), shoot length (17-61%), shoot fresh weight (47-169%) and shoot dry weight (up to 90%). In addition, a significant decrease in disease index (62-100%) was observed in different single and co-culture treatments. In conclusion, the studied two bacterial strains showed synergistic effects on wheat growth promotion and fungal growth inhibition. | ||
| کلیدواژه‌ها [English] | ||
| Azospirillum oryzae, Bacillus amyloliquefaciens, Biological control, Interaction, Synergistic effect | ||
| مراجع | ||
|
Ahmadi K, Qolizadeh H, Ebadzadeh H, Hosseinpour R, Abdshah H, Kazemian A, Rafiee M (2017) Crop Agri. Database 2015-2016.
Akinsanmi OA, Mitter V, Simpfendorfer S, Backhouse D, Chakraborty S (2004) Identity and pathogenicity of Fusarium spp. isolated from wheat fields in Queensland and northern New SouthWales. Austral. J. Agri. Res. 55: 97-107.
Bagheri N, Ahmadzadeh M, Ghasemi S, Vahidinasab M, Ghorshi Sh (2018) Bacillus amyloliquefaciens UTB96, a superior plant probiotic and aflatoxin- degrading bacterium. J. Bio. Plant Protect. In press.
Bashan Y, de-Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth a critical assessment. Adv. Agro. 108: 77-136.
Bockus WW, Bowden RL, Hunger RM, Morrill WL, Murray TD, Smiley RW (2010) Compendium of wheat diseases and pests, 3th Edition, APS.
Camacho M, Santamaria C, Temprano F, Rodriguez-Navarro DN, Daza A (2001) Co-inoculation with Bacillus sp. CECT 450 improves nodulation in Phaseolus vulgaris L. Can. J. Microbiol. 47: 11.
Carissimi M, Giraudo MS, Germani JC, Van Der Sand ST (2009) Antifungal activity of Bacillus sp. E164 against Bipolaris sorokiniana. Biociens. Porto. Alegre. 17 (1): 48-58.
Castillo P, Molina R, Andrade A, Vigliocco A, Alemano S, Cassan FD (2015) Phytohormones and other plant growth regulators produced by PGPR: the genus Azospirillum. In: Cassan FD, Okon Y, Creus CM (eds) Handbook for Azospirillum, Springer International Publishing Switzerland. 514pp.
CGIAR report, Research program on wheat, 2018 (https://wheat.org/wheat-in-the-world/)
Chakraborty S, Obanor F, Westecott R, Abeywickrama K (2010) Wheat crown rot pathogen Fusarium graminearum and F. pseudograminearum lacks specialisation. Phytopathol. 100: 1057-65.
Chen L, Heng J, Qin S, Bian K (2018) A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight. PLS. One. 13(6): e0198560.
Combes-Meynet E, Pothier JF, Moenne-Loccoz Y, Prigent-Combaret C (2011) The Pseudomonas secondary metabolite 2, 4-Diacetylphloroglucinol is a signal inducing rhizoplane expression of Azospirillum genes involved in plant-growth promotion. APS. 24 (2): 271-284.
Compant S, Duffy B, Nowak J, Clement C, Barka EA (2005) Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl. Envir. Microbiol. 71: 4951-4959.
Couillerot O, Ramirez-Trujillo A, Walker V, Felten AV, Jansa J, Maurhofer M, Defago G, Prigent-Combaret C, Comte G, Caballero-Mellado J, Moënne-Loccoz Y (2012) Comparison of prominent Azospirillum strains in Azospirillum–Pseudomonas–Glomus consortia for promotion of maize growth. Appl. Microbiol. Biotechnol. 97(10): 4639-4649.
Doornbos R, van Loon L, Bakker P (2012) Impact of root exudates and plant defense signaling on bacterial communities in the rhizosphere. Rev. Agro. Sustain. Develop. 32: 227–243.
El-Komy HMA (2005) Co immobilization of Azospirillum lipoferum and Bacillus megaterium for successful phosphorus and nitrogen nutrition of wheat plants. Food Technol. Biotechnol. 43 (1): 19–27.
Fernandez MR, Holzgang G, Turkington TK (2009) Common root rot and crown rot of barley crops across Saskatchewan and in north-central Alberta. Can. J. Plant Pathol. 31: 96-102.
Fernando WGD, Ramarathnama R, Krishnamoorthyb AS, Savchuka SC (2005) Identification and use of potential bacterial organic antifungal volatiles in biocontrol. Soil Biol. Bioche. 37: 955-964.
Grosu AI, Sicuia OA, Dobre A, Voaides C, Cornea CP (2015) Evaluation of some Bacillus spp. strains for the biocontrol of Fusarium graminearum and F. culmorum in wheat. Agri Sci. Procedia. 6: 559-566.
Hagedorn C, Gould WD, Bardinelli TR (1989) Rhizobacteria of cotton and their repression of seedling disease pathogens. Appl. Envir. Microbiol. 55: 2793- 2797.
Hassani MA, Duran P, Hacquard S (2018) Microbial interactions within the plant holobiont. Microb. 6:58.
Kanchana D, Jayanthi M, Usharani G, Saranraj P, Sujitha D (2014) Interaction Effect of Combined Inoculation of PGPR on Growth and Yield Parameters of Chilli Var K1 (Capsicum annuum L.). Inter. J. Microbiol. Res. 5 (3): 144-151.
Lounaci L, Guemouri-Athmani S, Boureghda H, Achouak W, Heulin T (2016) Suppression of crown and root rot of wheat by the rhizobacterium Paenibacillus polymyxa. Phytopathol. Med. 55 (3): 355−365.
Ma LJ, Geiser DM, Proctor RH, Rooney AP, O'Donnell K, Trail F, Kazan K (2013) Fusarium pathogenomics. An. Rev. Microbiol. 67: 399-416.
Marimuthu S, Subbian P, Ramamoorthy V, Samiyappan R (2002) Synergistic effect of combined application of Azospirillum and Pseudomonas fluorescence with inorganic fertilizer on root rot incidence and yield of cotton. J. Plant Dis. Protect. 109 (6): 569-577.
Perez-Garcia A, Romero D, de Vicente A (2011) Plant protection and growth stimulation by microorganisms: biotechnological applications of Bacilli in agriculture. Cur. Opinion Biotechnol. 22: 187–193.
Rojas A, Holguin G, Glick BR, Bashan Y (2001) Synergism between Phyllobacterium sp. (N2-fixer) and Bacillus licheniformis (P-solubilizer), both from a semiarid mangrove rhizosphere. FEMS Microbiol. Ecol. 35: 181-187.
Russo A, Felici C, Toffanin A, Gottz M, Collados C, Barea JM, Moenne-Loccoz Y, Smalla K, Vanderleyden J, Nuti M (2005) Effect of Azospirillum inoculants on arbuscolar mycorrhiza establishment in wheat and maize plants. Biol. Fertil. Soils. 41: 301-309.
Siah A, Deweer C, Morand E, Reignault P, Halama P (2010) Azoxystrobin resistance of French Mycosphaerella graminicola strains assessed by four in vitro bioassay and by screening of G143A substitution. Crop. Prot. 29:737-743.
Schisler DA, Khan NI, Boehm MJ, Slininger PJ (2002) Greenhouse and field evaluation of biological control of Fusarium head blight on durum wheat. Plant Dis. 86: 1350- 1356.
Wachowska U, Packa D, Wiwart M (2017) Microbial inhibition of Fusarium pathogens and biological modification of Trichothecenes in cereal grains. Toxins. 9: 408.
Wang Q, Buxa SV, Furch A, Friedt W, Gottwald S (2015) Insights into Triticum aestivum seedling root rot caused by Fusarium graminearum. Americ. Phytopathol. Soci. (APS).
Wasim M (2006) Role of chemotaxis genes in wheat root colonization by Azospirillum brasilense. Dissertation, Georgia State University.
Wiese MV, Murray TD, Forster RL (2000) Common names of plant diseases: Diseases of wheat. Americ. Phytophatol. Soci. (APS).
Yahalom E, Okom Y, Dovrat A (1990) Possible mode of action of Azospirillum brasilense strain Cd on the root morphology and nodule formation in burr medic (Medicago plymorpha). Can. J. Microbiol. 36: 10-4. | ||
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