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تأثیر نانو‌ذرات نقره بر شاخصهای ایمنی و آنزیمهای سرم خون ماهی شیربت (Barbus grypus) | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 1، دوره 7، شماره 1، شهریور 1397، صفحه 11-25 اصل مقاله (1.11 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2018.6156 | ||
| نویسندگان | ||
| بیتا سراج1؛ مصباح مهرزاد2؛ پریا اکبری* 1 | ||
| 1استادیار، گروه شیلات، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران | ||
| 2استادیار، گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| چکیده | ||
| قدرت باکتری کشی سرم در تیمارهای 1 درصد، 2 درصد و 4 درصد 50LC در روز 21 و در تیمار 8 درصد 50LC در روزهای 14 و 21 افزایش معنیداری را نسبت به تیمار شاهد نشان داد (05/0p<). همچنین میزان فعالیت لیزوزیم سرم در تیمار 4 درصد 50LC در روز 21 و در تیمار 8 درصد 50LC در روز 14و 21 افزایش معنیداری را نسبت به تیمار شاهد نشان داد (05/0p<). میزان آنزیمهای آلکالین فسفاتاز (ALP) و لاکتات دهیدروژناز (LDH)در تیمار 8 درصد 50LC در روز 21 افزایش معنیداری پیدا کرد (05/0p<). همچنین میزان آنزیمهای گلوتامیک اگزال استیک ترانس آمیناز (SGOT) و گلوتامیک پیروویک ترانس آمیناز (SGPT) در تیمار 2 درصد 50LC در روزهای 14 و 21 و در تیمارهای 4 و 8 درصد 50LC در روزهای 7، 14 و21 افزایش معنیداری نسبت به تیمار شاهد پیدا کرد (05/0p<). لذا میتوان نتیجه گرفت که غلظتهای تحت کشنده نانو ذرات نقره علاوه بر تأثیر مثبت بر روی شاخصهای ایمنی سبب افزایش آنزیمهای سرم خون ماهی شیربت میشوند. | ||
| کلیدواژه‌ها | ||
| آمینو ترانسفرازها؛ بیوشیمیایی؛ لیزوزیم؛ ماهی شیربت؛ نانو ذرات نقره | ||
| عنوان مقاله [English] | ||
| The effect of silver nanoparticles on the immune parameters and blood serum enzymes of farmed Shirbut (Barbus grypus) | ||
| نویسندگان [English] | ||
| Bita Seraj1؛ Mesbah Mehrzad2؛ Paria Akbari1 | ||
| 1Assistant Professor, Fisheries group, Marine Sciences, Chabahar Maritime University, Chabahar, Iran | ||
| 2Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran | ||
| چکیده [English] | ||
| In this study, in addition to the LC50 and the maximum allowable concentration, effects of silver nanoparticles on several of immune and blood serum enzymes parameters of Barbus grypus were determined For this purpose, 240 shirbut, to investigate the effects of sub lethal toxicity were divided as follows-control treatment (G1), the concentration LC50 1% (0.012 mg/L AgNP) (G2), concentration LC50 2% (0.025 mg/L AgNP) (G3) and concentration LC50 4% (0.05 MG/L AgNP) (G4) and concentration LC50 8% (0.1 Mg/ L AgNP) (G5). Then on days zero, 7, 14 and 21 immune responses and serum enzymes (ALT, LDH, SGOP, SGPT) in Barbus grypus were measured. In the study, WBC counts increased significantly (P<0.05) in G2, G3, G4 and G5 treatments compared to control (G1). Serum anti-bacterial activity was enhanced in G3 in twenty first day and G4 and G5 in 14th and 21s days in compared to control. Serum lysozyme activity enhanced significantly (P<0.05) in (G4) in 14th and (G5) 14th and 21s in days compared to control. ALT and LDH Level decreased significantly in (G2), (G3), (G4) and (G5) treatments in 21s day compared to control. SGPT level decreased significantly in (G2), (G3), (G4) and (G5) in 21s day compared to control. SGOT level decreased significantly in (G3), (G4) and (G5) treatments in 21s day compared to control. According to the results of this study concentrations toxicity of nano silver increased immune responses and serum enzymes. | ||
| کلیدواژه‌ها [English] | ||
| Aminotransferase, Barbus grypus, lysozyme, Silver nanoparticles | ||
| مراجع | ||
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