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وجود اینترلوکین 35 در پلاسمای منی: آیا مسئله مهمی در ناباروری مردان محسوب می شود؟ | ||
فصلنامه علمی زیست شناسی جانوری تجربی | ||
دوره 12، شماره 1 - شماره پیاپی 45، شهریور 1402، صفحه 45-55 اصل مقاله (923.07 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/eab.2023.68036.1911 | ||
نویسندگان | ||
ساناز علائی1، 2؛ محمود صادقی عطاآبادی3؛ حمیده همایون4؛ بهیه نام آور جهرمی4، 5؛ الهام حسینی* 6 | ||
1گروه بیولوژی تولیدمثل، دانشکده علوم و فن آوریهای نوین پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران | ||
2مرکز تحقیقات فناوری سلولهای بنیادی، دانشگاه علوم پزشکی شیراز، شیراز، ایران | ||
3گروه بیولوژی تولیدمثل، دانشکده علوم و فنآوریهای نوین پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران | ||
4گروه زنان و مامایی، دانشکده پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران | ||
5مرکز تحقیقات ناباروی، دانشگاه علوم پزشکی شیراز، شیراز، ایران | ||
6گروه زنان و زایمان، بیمارستان موسوی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران | ||
چکیده | ||
علت حدوداً نیمی از ناباروریها در زوجین مربوط به فاکتورهای مردانه است که میتواند به دلایل گوناگونی رخ دهد. بررسی متغیرهای پلاسمای منی اولین و سادهترین روش بررسی ناباروری در مردان است و اطلاعات ارزندهای از وضعیت باروری فرد در اختیار قرار میدهد. در این مطالعه، حضور و ارتباط میان میزان غلظت اینترلوکین 35 (IL-35) مایع منی با متغیرهای استاندارد اسپرم در مردان نابارور بررسی گردید. تجزیه و تحلیل مایع منی روی نمونههای منی مردان مراجعهکننده به مرکز درمان ناباروری حضرت زینب(س) دانشگاه علوم پزشکی شیراز انجام گردید. نمونههای منی بر اساس دستورالعمل آزمایشگاهی سازمان بهداشت جهانی بررسی و به سه گروه تقسیمبندی شدند که شامل 21 نمونه نرمال، 21 نمونه آستنوتراتوزواسپرمی و 15 نمونه آزواسپرمی بودند. حضور و سطح غلظت IL-35 پلاسمای منی در گروههای مورد مطالعه با استفاده از روش الایزا اندازهگیری شد. بررسی IL-35 بیانگر حضور این اینترلوکین در مایع منی هر سه گروه مورد مطالعه است، اما غلظت آن از نظر آماری تفاوت معنیدار نشان نداد (p>0.05). تجزیه و تحلیل همبستگی و ارتباط میان غلظت IL-35 با متغیرهای استاندارد اسپرم نشان داد که بین غلظت IL-35 با درصد مورفولوژی نرمال اسپرم همبستگی منفی معناداری وجود دارد (317/0-r=، 04/0p=)، درحالیکه همبستگی معنادار با تعداد اسپرم (27/0-r=، 08/0p=) و حرکت اسپرم (27/0-r=، 08/0p=) مشاهده نشد. با وجود اثبات حضور IL-35 در مایع منی حتی در مردان با متغیرهای نرمال، نقش و اهمیت عملکردی آن هنوز مشخص نشده است. وجود رابطه منفی بین غلظت IL-35 با درصد مورفولوژی نرمال اسپرم میتواند نشاندهنده اهمیت آن در توانایی باروری مردان باشد. | ||
کلیدواژهها | ||
اینترلوکین 35؛ پلاسمای منی؛ کیفیت اسپرم | ||
عنوان مقاله [English] | ||
The presence of Interleukin 35 in seminal plasma: is it a significant issue in male infertility? | ||
نویسندگان [English] | ||
Sanaz Alaee1، 2؛ Mahmoud Sadeghi Ataabadi3؛ Hamideh Homayon4؛ Bahia Namavar Jahromi4، 5؛ Elham Hosseini6 | ||
1Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran |Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
2Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran |Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
3Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran | ||
4Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
5Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran |Infertility Research Center, Zeynab Hospital, Shiraz University of Medical Sciences, Shiraz, Iran | ||
6Department of Obstetrics and Gynecology, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran | ||
چکیده [English] | ||
Approximately half of infertility in couples can be attributed to male factors, which may occur in a wide range of causes. Examining seminal plasma parameters is the initial and simplest approach in male infertility evaluation and provides valuable information about fertility status. In this study, the presence and relationship between the concentration of interleukin 35 (IL-35) in seminal plasma and standard parameters of sperm in infertile men were investigated. Semen analysis was performed on semen samples of men referred to the Infertility Center of Shiraz University of Medical Sciences. Semen samples were analyzed in accordance with the laboratory guidelines of the World Health Organization and were divided into three groups including 21 normozoospermic, 21 asthenoteratozoospermic, and 15 azoospermic samples. The seminal concentration level of IL-35 was analyzed using the enzyme-linked immunosorbent assay (ELISA) method. Results showed that IL-35 was detectable with different levels in the seminal plasma of studied groups, however, its concentration did not show a statistically significant difference (p>0.05). The correlation analysis between IL-35 concentration and sperm standard parameters showed that there is a significant negative correlation between IL-35 concentration and the normal sperm morphology percentage (r=0.317, p=0.04), while no significant correlation was observed with sperm count (r=-0.27, p=0.08) and sperm motility (r=-0.27, p=0.08). Despite the presence of IL-35 in semen, even in normozoospermic male patients, its role and functional significance have not been determined. The existence of a negative relationship between the IL-35 concentration and the normal sperm morphology percentage can indicate its importance in male infertility status. | ||
کلیدواژهها [English] | ||
Interleukin 35, Seminal plasma, Sperm quality | ||
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