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ارزیابی اثر ضد باکتریایی عصاره آویشن دنایی (Thymus daenensis) بر باکتریهای استرپتوکوکوس سالیواریوس، یرسینیا انتروکولیتیکا و شیگلا فلکسنری | ||
فصلنامه علمی زیست شناسی جانوری تجربی | ||
دوره 12، شماره 3 - شماره پیاپی 47، اسفند 1402، صفحه 73-82 اصل مقاله (923.16 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/eab.2024.69608.1929 | ||
نویسندگان | ||
رضا یدی1؛ فاطمه حق شناس گتابی1؛ نعیمه دهقانی* 2؛ حامد دانش پژوه3 | ||
1گروه کشاورزی، دانشگاه پیام نور، تهران، ایران. صندوق پستی 3697-19395 | ||
2گروه زیستشناسی، دانشگاه پیام نور، تهران، ایران | ||
3گروه زیست شناسی، دانشکده علوم پایه،دانشگاه پیام نور مرکز بوشهر، ایران | ||
چکیده | ||
بهدلیل مقاومت روز افزون باکتریهای بیماریزا به آنتیبیوتیکهای جدید پژوهشگران در پی یافتن مواد ضدمیکروبی با منشأ گیاهی بهعنوان جایگزین آنتیبیوتیکهای غیر مؤثر هستند. هدف از انجام این مطالعه ارزیابی اثر ضدباکتریایی عصاره هیدروالکلی گیاه آویشندنایی بر روی باکتریهای شیگلا فلکسنری، یرسینیا انتروکولیتیکا و استرپتوکوکوس سالیواریوس میباشد. روش بررسی: در این مطالعه تجربی، گیاه آویشندنایی جمعآوری و عصاره گیاه بهروش خیساندن (ماسراسیون) تهیه و اثرات ضد میکروبی این گیاهان در رقتهای 65/0، 25/1، 5/2، 5 و 10میلیگرم بر لیتر با استفاده از روش انتشار در دیسک بر روی باکتریهای شیگلا فلکسنری، یرسینیا انتروکولیتیکا و استرپتوکوکوس سالیواریوس در مقایسه با آنتیبیوتیکهای پنیسیلین، جنتامایسین، تتراسایکلینو ونکومایسین تعیین و قطر هاله عدم رشد اندازهگیری شد. همچنین حداقل غلظت مهار کننده رشد (MIC) و حداقل غلظت کشندگی (MBC) عصارهها بر علیه سه باکتری موردنظر تعیین گردید. یافته ها: نتایج بهدستآمده نشان داد که عصاره گیاه آویشن بر استرپتوکوکوس سالیواریوس در غلظت 10 میلیگرم بر لیتر بیشترین اثر را داشته و قطر هاله عدم رشد 33/20 اندازهگیری گردید. هم چنین این عصارهها بر روی باکتری یرسینیا هیچ اثری نداشتند. برای اثرمتقابل آنتیبیوتیک در باکتری حداکثر قطر هاله عدم رشد برای آنتیبیوتیک تتراسایکلین و باکتری استرپتوکوکوس بهدست آمد، اما در اثر متقابل آنتیبیوتیک ونکومایسین و باکتری یرسینیا هالهای تشکیل نشد. مقدار MIC مربوط به گیاه روی باکتریهای استرپتوکوکوس سالیواریوس و شیگلا فلکسنری مقدار مشابهی را نشان داد که برابر 65/0 میلیگرم بر لیتر بود و مقدار MIC روی باکتری یرسینیا انتروکولیتیکا برابر25/1 میلیگرم برلیتر بهدست آمد. همچنین مقدار مؤلفه MBC برای گیاه آویشن دنایی روی باکتری یرسینیا و شیگلا برابر 5/2 میلیگرم برلیتر و برای باکتری استرپتوکوکوس سالیواریوس برابر 25/1میلیگرم بر لیتر بهدست آمد. نتیجه گیری: بنابراین، عصاره هیدرو الکلی گیاه آویشن دارای اثر بازدارندگی بوده و این عصاره بر باکتری استرپتوکوکوس اثر بیشتری نسبت به دو باکتری یرسینیا و شیگلا نشان میدهد. | ||
کلیدواژهها | ||
آویشن دنایی؛ شیگلا فلکسنری؛ یرسینیا انتروکولیتیکا؛ استرپتوکوکوس سالیواریوس | ||
عنوان مقاله [English] | ||
Evaluation of the antibacterial effect of Danai thyme extract on Streptococcus salivarius, Yersinia enterocolitica and Shigella flexneri bacteria | ||
نویسندگان [English] | ||
Reza Yadi1؛ Fatemeh Haghshenas Gatabi1؛ Naeimeh Dehghani2؛ Hamed Daneshpazhouh3 | ||
1Department of Agriculture, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran | ||
2Department of Biology, Payame Noor University, Tehran, Iran | ||
3Department of Biology, Faculty of Basic Sciences, Payam Noor University, Bushehr Center, Iran | ||
چکیده [English] | ||
Introduction: Due to increasing resistance of pathogenetic bacteria to the new antibiotics, the researchers are seeking of finding the antimicrobial materials with herbal origins as the replacement of ineffective antibiotics. The aim of doing of this study is evalution of the antibacterial effect of hydro alcoholic extraction of the plant of thymus daensis on the bacteria of shigella flexneri, Yersinia enterocolitica and streptococcus salivarius. Investigation method: in this practical study, the plant of thymus daensis were collected and the extraction of plant were prepared by the method of maceration and the antimicrobial effects of these plant in the amounts of 0.65, 1.25, 2.5, 5 and 10 mg/l were prepared by using the method of transmission on disk on the bacteria of shigella flexneri, Yersinia enterocolitica and Streptococcus salivarius compared with the antibiotics of Penicillin, Gentamicin, Tetracycline and Vancomycin and drop of halo of lack of growth was measured. Also the minimum concentration of restraining of growth (MIC) and the minimum concentration of lethalithy (MBC) of extractions against three concerned bacteria were determined. Results: The obtained results showed that the extraction of thymus daensis on streptococcus salivarius in the concentration of 10mg/l had the most effect and the drop of halo of lack of growth were respectively measured as 20.83, 20.33, 19. Also this extractions didn’t have any effect on the bacterium of Yersinia for mutuol effect of antibiotic in bacterium the maximum drop of halo of lack of growth for antibiotic of Tetracycline and bacterium of Streptococcus salivarius was achived but in the mutual effect of antibiotic of Vancomycin and the bacterium of Yersinia any halo wasn’t composed. The amount of mic related to thymus daensis on the bacteria of Streptococcus salivarius and Shigella flexneri showed a similar amount the equals to 0.65 mg/l and the amount of mic related to thymus daensis on the bacterium of Yersinia enterocolitica was achieved equals to1.25 mg/l. the amount of mbc component for the plant of Thymus daensis on the bacteria of Yersinia and Shigella was achived equals to 2.5 mg/l and for bacteria of Streptococcus salivarius was achived equals to 1.25 mg/l . Conclusion: There for, the hydro-alcoholic extraction of thymus daensis has an inhibitory effect, this extractions showed more effect on the bacterium of Streptococcus than two bacteria Yersinia and Shigella. | ||
کلیدواژهها [English] | ||
Thymus daensis, Shigella flexneri, Yersinia enterocolitica, Streptococcus salivarius | ||
مراجع | ||
Akbarinia, A., Sharifi Ashoorabadi, E., & Mirza, M. A. H. D. I. (2010). Study on drug yield and essential oil content and composition of Thymus daenensis Celak. under cultivated condition. Iranian Journal of Medicinal and Aromatic Plants Research, 26(2), 205-212.
Bonardi, S., Bassi, L., Brindani, F., D'Incau, M., Barco, L., Carra, E., & Pongolini, S. (2013). Prevalence, characterization and antimicrobial susceptibility of Salmonella enterica and Yersinia enterocolitica in pigs at slaughter in Italy. International Journal of Food Microbiology, 163(2-3), 248-257.
Bagamboula, C. F., Uyttendaele, M., & Debevere, J. (2004). Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food microbiology, 21(1), 33-42.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods-a review. International journal of food microbiology, 94(3), 223-253.
Castor, M. L., Whitney, C. G., Como-Sabetti, K., Facklam, R. R., Ferrieri, P., Bartkus, J. M., ... & Lynfield, R. (2009). Antibiotic resistance patterns in invasive group B streptococcal isolates. Infectious diseases in obstetrics and gynecology, 2008.
Corrêa, A. B. D. A., Silva, L. G. D., Pinto, T. D. C. A., Oliveira, I. C. M. D., Fernandes, F. G., Costa, N. S. D., ... & Benchetrit, L. C. (2011). The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, 106, 1002-1006.
Dakhili, M., Zahraei Salehi, T., Torabi Goodarzi, M., & Khavari, A. (2006). Evaluation of antimicrobial effects of 4 medicinal plants against Salmonella typhymurium and comparision them with common antibiotics in veterinary medicine. Journal of Medicinal Plants, 5(20), 21-26.
Fazeli, M. R., Amin, G., Attari, M. M. A., Ashtiani, H., Jamalifar, H., & Samadi, N. (2007). Antimicrobial activities of Iranian sumac and avishan-e shirazi (Zataria multiflora) against some food-borne bacteria. Food control, 18(6), 646-649.
Fujita, M., Shiota, S., Kuroda, T., Hatano, T., Yoshida, T., Mizushima, T., & Tsuchiya, T. (2005). Remarkable synergies between baicalein and tetracycline, and baicalein and β‐lactams against methicillin‐resistant Staphylococcus aureus. Microbiology and immunology, 49(4), 391-396.
Gandomi, H., Misaghi, A., Basti, A. A., Bokaei, S., Khosravi, A., Abbasifar, A., & Javan, A. J. (2009). Effect of Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese. Food and chemical toxicology, 47(10), 2397-2400.
Goudarzi, M., Sattari, M., Najar piraieh, S., Goudarzi, G. & Bigdeli, M. (2006). Antibacterial effects of aqueous and alcoholic extracts of Thyme on enterohemorrhagic Escherichia coli. Yafte, 8(3), 63-69. (in Persian)
Gradwohl, R. B. H. (1948). Clinical Laboratory Methods and Diagnosis, CV Mosby Co. St. Louis, Missouri, 1, 574-654.
Hanifian, S., & Khani, S. (2012). Prevalence of virulent Yersinia enterocolitica in bulk raw milk and retail cheese in northern-west of Iran. Int J Food Microbiol, 155, 85-92.
Javan, A.J. (2016). Combinational effects of Trachyspermum ammi and Zataria multiflora Boiss essential oils on some pathogenic food-borne bacteria. Koomesh, 17(2).
Jawets, A. (2014). Medical microbiology. Translated by H. Jahandideh., H. Jahandideh, Isfahan: Sena. (in Persian)
Kandian, S., Sattari, M., Nikbin, V., & Aslani, M. M. (2018). Examining the pattern of antibiotic sensitivity and determining the ipaH gene in Shigella strains isolated from selected provinces of the country. Madras Journal of Medical Sciences biological damage, 14 (1), 81-88. (in Persian)
Karaman, S., Digrak, M., Ravid, U., & Ilcim, A. (2001). Antibacterial and antifungal activity of the essential oils of Thymus revolutus Celak from Turkey. Journal of ethnopharmacology, 76(2), 183-186.
Khosravipour, S., & Rezaeian-Doloei, R. (2015). Antibacterial activity of Carum copticum essential oil against Pectobacterium carotovorum subsp. carotovorum and Escherichia coli in nutrient broth medium.
Kowalczyk, A., Przychodna, M., Sopata, S., Bodalska, A., & Fecka, I. (2020). Thymol and thyme essential oil-new insights into selected therapeutic applications. Molecules, 25(18), 4125.
Mahboubi, M. O., & Feizabadi, M. M. (2009). The antimicrobial activity of Thyme, Sweet Marjoram, Savory and Eucalyptus oils on Escherichia coli, Salmonella typhimurium, Aspergillus niger and Aspergillus flavus. Journal of Medicinal Plants, 8(30), 137-144.
Mahboubi, M., Heidarytabar, R., Mahdizadeh, E., & Hosseini, H. (2017). Antimicrobial activity and chemical composition of Thymus species and Zataria multiflora essential oils. Agriculture and natural resources, 51(5), 395-401.
Masoumian, M., & Zandi, M. (2017). Antimicrobial activity of some medicinal plant extracts against multidrug resistant bacteria. Zahedan Journal of Research in Medical Sciences, 19(11).
Miladi, H., Slama, R. B., Mili, D., Zouari, S., Bakhrouf, A., & Ammar, E. (2013). Essential oil of Thymus vulgaris L. and Rosmarinus officinalis L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens.
Momtaz, H., Farzan, R., Rahimi, E., Safarpoor Dehkordi, F., & Souod, N. (2012). Molecular characterization of Shiga toxin-producing Escherichia coli isolated from ruminant and donkey raw milk samples and traditional dairy products in Iran. The Scientific World Journal, 2012.
Omid Beigi, R. (2000). Approaches to the production and processing of medicinal plants. Tehran: Mosharan Nash Publications. (in Persian)
Omidbeigi, R. (1997). Approaches to the production and processing of medicinal plants. Tehran: Designers Publishing House. (in Persian)
Parsaeimehr, M., Akhondzade Basti, A., Misaghi, A., Zahraei Salehi, T., Radmehr, B., & Gandomi Nasrabadi, H. (2010) Effect of Zataria multiflora Boiss. Essential Oil on Enterotoxin Production by Staphylococcus aureus ATCC 6538. J. Med. Plants, 9(33), 98-102. (in Persian)
Peirano, G., Souza, F. D. S., & Rodrigues, D. D. P. (2006). Frequency of serovars and antimicrobial resistance in Shigella spp. from Brazil. Memorias do Instituto Oswaldo Cruz, 101, 245-250.
Rahimi, E., Sepehri, S., Safarpoor, F., Shaygan, S., & Momtaz, H. (2014). Prevalence of Yersinia species in traditional and commercial dairy products in Isfahan province, Iran. Jundishapur J Microbiol, 7(4), e9249. Ranjbar, R., Seif, A., & Dehkordi, F. S. (2019). Prevalence of antibiotic resistance and distribution of virulence factors in the shiga toxigenic Escherichia coli recovered from hospital food. Jundishapur Journal of Microbiology, 12(5).
Ranjbar, R., Yadollahi Farsani, F., & Safarpoor Dehkordi, F. (2019). Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products. Journal of Food Safety, 39(2), e12594.
Rasouli, B. (1998). Investigating the antimicrobial effects of thyme and purple spike from the mint family, sumac and pistachio tuber by in vitro method. Master thesis of Urmia University. (in Persian)
Rechinger, K. H., & Iranica F. (1982). Akademische Druck-U: Verlagsnstalt GRAZ, 150, 532-551.
Safarpoor Dehkordi, F., Gandomi, H., Basti, A. A., Misaghi, A., & Rahimi, E. (2017). Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food. Antimicrobial resistance & infection control, 6(1), 1-11.
Seo, Y. S., Srinivasan, U., Oh, K. Y., Shin, J. H., Chae, J. D., Kim, M. Y., ... & Ki, M. (2010). Changing molecular epidemiology of group B streptococcus in Korea. Journal of Korean medical science, 25(6), 817-823.
Shahnia, M., & Khaksar, R. (2013). Antimicrobial effects and determination of minimum inhibitory concentration (MIC) methods of essential oils against pathogenic bacteria. Iranian Journal of Nutrition Sciences & Food Technology, 7(5), 949-955.
Sheeladevi, A., & Ramanathan, N. (2012). Antibacterial activity of plant essential oils against food borne bacteria. International Journal of Pharmaceutical & Biological Archives, 3(5), 1106-1109.
Soltan Dallal, M. M., Bayat, M., Yazdi, M. H., Aghaamiri, S., Ghorbanzadeh Meshkani, M., Abedi Mohtasab, T. P., & Shojaee Sadi, B. (2012). Antimicrobial effect of Zataria multiflora on antibiotic-resistant Staphylococcus aureus strains isolated from food. Scientific Journal of Kurdistan University of Medical Sciences, 17(2), 21-29.
Soltani Nejad, S., Mokhtari, T., & Rahbarian, P. (2010). Antibacterial effect of Ziziphora clinopodiodes essential oil and methanol extract on pathogen bacteria. J. Mic. Bio. Tech. of Azad Islamic University, 2(5), 1-6.
Tajkarimi, M. M., Ibrahim, S. A., & Cliver, D. O. (2010). Antimicrobial herb and spice compounds in food. Food control, 21(9), 1199-1218.
Zahrai Salehi, T., Vejgani, M., Bayat, M., Tarshizi, H., & Akhundzadeh, M. (2013). Determining the minimum inhibitory concentration (MIC) of Shirazi thyme essential oil on Staphylococcus aureus, Streptococcus agalitae and Escherichia coli bacteria. Journal of Faculty of Veterinary Medicine, University of Tehran, 6(2), 110-107. (in Persian)
Zc N, ra ić , Fi i ić I. (2015). Prac Salmonella spp. and Yersinia enterocolitica in/on tonsils and mandibular lymph nodes of slaughtered pigs. Folia Microbiol, 60: 131-135. | ||
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