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تأثیر کامپفرول از فلاونوئیدهای گیاه بابونه (Matricaria recutita) بر روی اختلالات حافظه و استرس اکسیداتیو ناشی از اثر استرپتوزوتوسین در موش‌های صحرایی نر بالغ | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 12، شماره 4 - شماره پیاپی 48، اردیبهشت 1403، صفحه 53-64 اصل مقاله (1.4 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2024.70052.1933 | ||
| نویسنده | ||
| سید رضا پورربی* | ||
| گروه زیست‌شناسی، واحد مرند، دانشگاه آزاد اسلامی، مرند، ایران | ||
| چکیده | ||
| بیماری آلزایمر یکی از شایع‌ترین بیماری‌های از بین برنده نورونی است. پلاک‌های بتاآمیلوئیدی و کلافهای نوروفیبریلاری دو عامل مهم در بیماری آلزایمر هستند تغییرات سلولی زیادی، نظیر استرس اکسیداتیو، التهاب نورونی و اختلالات میتوکندریایی نیز در آلزایمر دیده می‌شوند که منجر به مرگ نورونی می‌شوند. کامپفرول از فلاونوئیدهای گیاهی مشتق‌شده از گیاه بابونه به‌خاطر تولید آنتی‌اکسیدان‌ها، دارای اثرات مهم بر روی اختلالات مربوط به بیماری‌های مغزی نظیر بیماری آلزایمر می‌باشد. در این مطالعه اثرات فلاونوئیدهای گیاهی بابونه بر روی اختلالات حافظه در موش‌های صحرایی آلزایمری شده موردمطالعه قرار گرفت. بدین منظور از عصاره هیدروالکلی بابونه‌های جمع‌آوری شده و آنالیز کمی- کیفی مواد شیمیایی عصاره بابونه با استفاده از روش کروماتوگراف گازی متصل به اسپکترومتر جرمی (GC/MS) جهت تهیه و تخلیص کامپفرول استفاده شد. در این مطالعه از 56 موش صحرایی نر بالغ که به هفت گروه شامل کنترل- حامل 1 (حلال کامپفرول)- حامل 2 (حلال داروی استرپتوزوتوسین)- آلزایمر- کامپفرول mg/kg120- کامپفرول mg/kg250- کامپفرول mg/kg400- مورداستفاده قرار گرفت. القای دیابت با تزریق تک‌دوز mg/kg60 داروی استرپتوزوتوسین به‌صورت درون صفاقی صورت گرفت. تجویز کامپفرول تا 15 روز انجام و برای سنجش میزان حافظه و یادگیری از دستگاه شاتل باکس استفاده شد و مدت زمان تأخیر در ورود شاتل باکس ثبت گردید. نتایج با استفاده از نرم‌افزار 22 SPSS و به‌کمک آزمون‌های ANOVA و توکی آنالیز شدند. معنی‌داری اختلاف داده‌ها در سطح 05/0 P≤ در نظر گرفته شد. نتایج حاصل از آنالیز داده‌ها نشان داد دوزهای mg/kg 400 و 250 از کامپفرول گیاه بابونه باعث افزایش معنی‌دار مدت زمان تأخیر در ورود به بخش تاریک در سطح 001/0P≤ نسبت به گروه کنترل شدند و باعث بهبود حافظه فضایی در موش‌های صحرایی شد. هم‌چنین پارامترهای استرس اکسیداتیو در گروه‌های آلزایمری تیمارشده با کامپفرول گیاه بابونه کاهش معنی‌داری یافت (001/0P<). نتایج آزمایشات نشان می‌دهند که فلاونوئیدهای گیاهی قادر به بازگرداندن عملکرد حافظه فضایی و مقدار پارامترهای استرس اکسیداتیو گروه‌های تحت تیمار با داروی استرپتوزوتوسین به سطح طبیعی است. | ||
| کلیدواژه‌ها | ||
| آلزایمر؛ کامپفرول؛ یادگیری؛ استرس اکسیداتیو؛ موش صحرایی | ||
| عنوان مقاله [English] | ||
| The effect of kaempferol from flavonoids of chamomile plant (Matricaria recutita) on memory disorders and oxidative stress caused by streptozotocin effect in adult male rats | ||
| نویسندگان [English] | ||
| Seyed Reza Pourrabie | ||
| Department of Biology, Islamic Azad University, Marand Branch Iran | ||
| چکیده [English] | ||
| Alzheimer's disease is one of the most common neurodegenerative diseases. Beta-amyloid plaques and neurofibrillary tangles are two important factors in Alzheimer's disease. Many cellular changes, such as oxidative stress, neuronal inflammation, and mitochondrial disorders are also seen in Alzheimer's disease, which lead to neuronal death. Kaempferol, one of the plant flavonoids derived from the chamomile plant, has important effects on disorders related to brain diseases such as Alzheimer's disease due to the production of antioxidants.In this study, the effects of chamomile plant flavonoids on memory disorders in Alzheimer's rats were studied. For prepare and purify kaempferol, the hydroalcoholic extract of the collected chamomiles, and quantitative-qualitative analysis of the chemical substances of the chamomile extract using gas chromatograph connected to mass spectrometer (GC/MS) method were used. In this study, we used of 56 adult male rats were divided into 7 groups including control - vehicle 1 (kaempferol solvent) - vehicle 2 (streptozotocin drug solvent) - Alzheimer's - kaempferol 120mg/kg - kaempferol 250mg/kg - kaempferol 400mg/kg. Diabetes was induced by injecting a single dose of 60 mg/kg streptozotocin intraperitoneally. Kaempferol were administered for 15 days. The shuttle box device was used to measure memory and learning, and the delay time in the shuttle box was recorded. The results were analyzed by SPSS 22 software, ANOVA and Tokay tests. The significance of the data difference was considered at the p ≤ 0.05 levels. The results of the data analysis showed that the doses of 400 mg/kg and 250 mg/kg of kaempferol of chamomile plant significantly increased the delay time in entering the dark section at p≤0.001 compared to the control group and improved spatial memory in rats. Which does this effect is probably due to the presence of compounds such as quercetin and phytoestrogens. Also, oxidative stress parameters were significantly reduced in Alzheimer's groups treated with chamomile flavonoids (P < 0.001) . The results of the experiments show that plant flavonoids are able to restore the spatial memory and oxidative stress parameters of the groups treated with streptozotocin to normal levels. | ||
| کلیدواژه‌ها [English] | ||
| Alzheimer, kaempferol, learning, Oxidative stress, rat | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 83 تعداد دریافت فایل اصل مقاله: 47 |
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