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تأثیر کامپفرول از فلاونوئیدهای گیاه بابونه (Matricaria recutita) بر روی اختلالات حافظه و استرس اکسیداتیو ناشی از اثر استرپتوزوتوسین در موشهای صحرایی نر بالغ | ||
فصلنامه علمی زیست شناسی جانوری تجربی | ||
دوره 12، شماره 4 - شماره پیاپی 48، اردیبهشت 1403، صفحه 53-64 اصل مقاله (1.4 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/eab.2024.70052.1933 | ||
نویسنده | ||
سید رضا پورربی* | ||
گروه زیستشناسی، واحد مرند، دانشگاه آزاد اسلامی، مرند، ایران | ||
چکیده | ||
بیماری آلزایمر یکی از شایعترین بیماریهای از بین برنده نورونی است. پلاکهای بتاآمیلوئیدی و کلافهای نوروفیبریلاری دو عامل مهم در بیماری آلزایمر هستند تغییرات سلولی زیادی، نظیر استرس اکسیداتیو، التهاب نورونی و اختلالات میتوکندریایی نیز در آلزایمر دیده میشوند که منجر به مرگ نورونی میشوند. کامپفرول از فلاونوئیدهای گیاهی مشتقشده از گیاه بابونه بهخاطر تولید آنتیاکسیدانها، دارای اثرات مهم بر روی اختلالات مربوط به بیماریهای مغزی نظیر بیماری آلزایمر میباشد. در این مطالعه اثرات فلاونوئیدهای گیاهی بابونه بر روی اختلالات حافظه در موشهای صحرایی آلزایمری شده موردمطالعه قرار گرفت. بدین منظور از عصاره هیدروالکلی بابونههای جمعآوری شده و آنالیز کمی- کیفی مواد شیمیایی عصاره بابونه با استفاده از روش کروماتوگراف گازی متصل به اسپکترومتر جرمی (GC/MS) جهت تهیه و تخلیص کامپفرول استفاده شد. در این مطالعه از 56 موش صحرایی نر بالغ که به هفت گروه شامل کنترل- حامل 1 (حلال کامپفرول)- حامل 2 (حلال داروی استرپتوزوتوسین)- آلزایمر- کامپفرول mg/kg120- کامپفرول mg/kg250- کامپفرول mg/kg400- مورداستفاده قرار گرفت. القای دیابت با تزریق تکدوز mg/kg60 داروی استرپتوزوتوسین بهصورت درون صفاقی صورت گرفت. تجویز کامپفرول تا 15 روز انجام و برای سنجش میزان حافظه و یادگیری از دستگاه شاتل باکس استفاده شد و مدت زمان تأخیر در ورود شاتل باکس ثبت گردید. نتایج با استفاده از نرمافزار 22 SPSS و بهکمک آزمونهای ANOVA و توکی آنالیز شدند. معنیداری اختلاف دادهها در سطح 05/0 P≤ در نظر گرفته شد. نتایج حاصل از آنالیز دادهها نشان داد دوزهای mg/kg 400 و 250 از کامپفرول گیاه بابونه باعث افزایش معنیدار مدت زمان تأخیر در ورود به بخش تاریک در سطح 001/0P≤ نسبت به گروه کنترل شدند و باعث بهبود حافظه فضایی در موشهای صحرایی شد. همچنین پارامترهای استرس اکسیداتیو در گروههای آلزایمری تیمارشده با کامپفرول گیاه بابونه کاهش معنیداری یافت (001/0P<). نتایج آزمایشات نشان میدهند که فلاونوئیدهای گیاهی قادر به بازگرداندن عملکرد حافظه فضایی و مقدار پارامترهای استرس اکسیداتیو گروههای تحت تیمار با داروی استرپتوزوتوسین به سطح طبیعی است. | ||
کلیدواژهها | ||
آلزایمر؛ کامپفرول؛ یادگیری؛ استرس اکسیداتیو؛ موش صحرایی | ||
عنوان مقاله [English] | ||
The effect of kaempferol from flavonoids of chamomile plant (Matricaria recutita) on memory disorders and oxidative stress caused by streptozotocin effect in adult male rats | ||
نویسندگان [English] | ||
Seyed Reza Pourrabie | ||
Department of Biology, Islamic Azad University, Marand Branch Iran | ||
چکیده [English] | ||
Alzheimer's disease is one of the most common neurodegenerative diseases. Beta-amyloid plaques and neurofibrillary tangles are two important factors in Alzheimer's disease. Many cellular changes, such as oxidative stress, neuronal inflammation, and mitochondrial disorders are also seen in Alzheimer's disease, which lead to neuronal death. Kaempferol, one of the plant flavonoids derived from the chamomile plant, has important effects on disorders related to brain diseases such as Alzheimer's disease due to the production of antioxidants.In this study, the effects of chamomile plant flavonoids on memory disorders in Alzheimer's rats were studied. For prepare and purify kaempferol, the hydroalcoholic extract of the collected chamomiles, and quantitative-qualitative analysis of the chemical substances of the chamomile extract using gas chromatograph connected to mass spectrometer (GC/MS) method were used. In this study, we used of 56 adult male rats were divided into 7 groups including control - vehicle 1 (kaempferol solvent) - vehicle 2 (streptozotocin drug solvent) - Alzheimer's - kaempferol 120mg/kg - kaempferol 250mg/kg - kaempferol 400mg/kg. Diabetes was induced by injecting a single dose of 60 mg/kg streptozotocin intraperitoneally. Kaempferol were administered for 15 days. The shuttle box device was used to measure memory and learning, and the delay time in the shuttle box was recorded. The results were analyzed by SPSS 22 software, ANOVA and Tokay tests. The significance of the data difference was considered at the p ≤ 0.05 levels. The results of the data analysis showed that the doses of 400 mg/kg and 250 mg/kg of kaempferol of chamomile plant significantly increased the delay time in entering the dark section at p≤0.001 compared to the control group and improved spatial memory in rats. Which does this effect is probably due to the presence of compounds such as quercetin and phytoestrogens. Also, oxidative stress parameters were significantly reduced in Alzheimer's groups treated with chamomile flavonoids (P < 0.001) . The results of the experiments show that plant flavonoids are able to restore the spatial memory and oxidative stress parameters of the groups treated with streptozotocin to normal levels. | ||
کلیدواژهها [English] | ||
Alzheimer, kaempferol, learning, Oxidative stress, rat | ||
مراجع | ||
Agrawal, R., Tyagi E., Shukla, R., & Nath, C. (2009). A Study of brain insulin receptors, AChEactivity and oxidative stress in rat model of ICV STZ induced dementia. Neuropharmacology, 56, 779-787.
Akbari, F., Ansari-Samani, R., Karimi, A., Mortazaei, S., Shahinfard, N., & Rafieian-kopaei, M. (2013). Effect of turnip on glucose and lipid profiles of alloxan-induced diabetic rats. Iran J Endocrinol Metab, 14(5), , 492-7. [In Persian]
Asadi, S.Y., Parsaei, P., Karimi, M., Ezzati, S., Zamiri, A., Mohammadizadeh, F., et al. (2013). Effect of green tea (Camellia sinensis) extract on healing process of surgical wounds in rat. Int J Surg, 11 (4), 332-7.
Aslan, M., & Ozben, T. (2004). Reactive oxygen and nitrogen species in Alzheimer's disease. Curr Alzheimer Res, 1(2), 111-9.
Azadmehr, A., Hajiaghaee, R., Afshari, A., Amirghofran, Z., Refieian-Kopaei, M., Yousofi-Darani, H, et al. (2011). Evaluation of in vivo immune response activity and in vitro anti-cancer effect by Scrophularia megalantha. J Med Plants Res, 5(11), 2365-8.
Bassett, C.N., & Montine, T.J. (2003). Lipoproteins and lipid peroxidation in Alzheimer's disease. J Nutr Health Aging, 7(1), , 24-9.
Becker, D.J., & Henquin, J.C. (1994). Comparison of the effects of various vanadium salts on glucose homeostasis instreptozotocin-diabetic rats. Eur J Pharmacol, 260(2-3), 169-75.
Behl, C. (2005). Oxidative stress in Alzheimer's disease, Implications for prevention and therapy. Subcell Biochem, 38, 65-78. [DOI, 10.1007/0-387-23226-5_3] [PMID]
Benzie, I.F., & Strain, J.J. (1999). Ferric reducing/antioxidant power assay, Direct measure of total antioxidant activity of biological fluids and modified version forsimultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 31(299), 15-27.
Brock, O., Douhard, Q., Baum, M. J., & Bakker, J. (2010). Reduced prepubertal expression of progesterone receptor in the hypothalamus of female aromatase knockout mice. Endocrinology 151, 1814-1821. doi, 10.1210/en.2009-1379
Calsolaro, V., & Edison, P. (2016), Neuroinflammation in Alzheimer's disease, current evidence and future directions. Alzheimers Dement, 12(6), 719-32.
Cao, G., & Prior, R.L. (1998 ). Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clinical chemistry, 1;44(6), , 1309-15.
Chandrashekhar, V.M., Ranpariya, V.L., Ganapaty, S., Parashar, A., & Muchandi, A.A. (2010). Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats. J Ethnopharmacol, 127(3), 645-51.
Duelli, R., Schrock, H., Kuschinsky, W., & Hoyer S. (1994). Intracerebroventricular injection of streptozotocin induces discrete local changes in cerebral glucose utilization in rats. Int. J. Dev Neurosci, 12, 737-743.
ElAli, A., & Rivest, S. (2016). Microglia in Alzheimer’s disease, a multifaceted relationship. Brain Behav Immun, 55, 138-50.
Esteban-Santillan, C., Praditsuwan, R., Ueda, H., et al. (1998). Clock drawing test in very mild Alzheimer’s disease. J Am Geriatr Soc, 46, 1266-1269.
Gomaa, A., Hashem, T., Mohamed, M., & Ashry, E. (2003). Matricaria chamomilla extract inhibits both development of morphine dependence and expression of abstinence syndrome in rats. J Pharmacol Sci, 92(1), 50-55.
Grünblatt, E., Koutsilieri, E., Hoyer, S., & Riederer, P., (2006). Gene expression alterations in brain areas of intracerebroventricular streptozotocin treated rat. J. Alzheimers Dis, 9, 261-271.
Gu, M., Owen, A., Toffa, S., Cooper, J.M., Dexter, D.T., Jenner, P., et al. (1998). Mitochondrial function, GSH and iron in neurodegeneration and Lewy body diseases. J Neurol Sci, 158(1), 24-9.
Hardy, J., & Selkoe, D.J. (2002). The amyloid hypothesis of Alzheimer’s disease, progress and problems on the road to therapeutics. Science, 297, 353-356.
Hayashi, T., Nozawa, M., Sohmiya, K., Toko, H., Nakao, M., Okabe, M., et al.( 1998). Efficacy of pancreatic transplantation on cardiovascular alterations in diabetic rats, an ultrastructural and immunohistochemical study. Transplant Proc, 30(2), 335-8.
Höglund, K., & Salter, H. (2013). Molecular biomarkers of neurodegeneration. Expert Rev Mol Diagn, 13(8), 845-61.
Honari, N., Pourabolli, I., Hakimi zadeh, E., Shamsizadeh, A., Amin, F., & Allatavakoli, M. (2012). Effect of Vitex Agnus-Castus Extract on Learning and Memory in Ovariectomized Rats. J Mazandaran Univ Med Sci, 22 (93), 2-10
Hosseinpour, M., Mobini-Dehkordi, M., Saffar, B., & Teimori, H. (2013). Antiproliferative effects of Matricaria chamomilla on Saccharomyces cerevisiae. J Herb Med Pharmacol, 2(2), 49-51.
Karimi, M., Parsaei, P., Asadi, S.Y., Ezzati, S., Khadivi Boroujeni, R., Zamiri, A., et al. (2013). Effects of Camellia sinensis ethanolic extract on histometric and histopathological healing process of burn wound in rat. Middle-East J Sci Res, 13(1), 14-9.
Kesmati, M., Raei, H., & Zadkarami, M. (2007). Effect of Matricaria chamomilla hydroalcolic extract on locomotor activity in the presence and absence of sex gland in male and female adult mice. Shahid Chamran University Journal of Science, 17(1), 73-83.
Khan, H., Ullah, H., Aschner, M., Cheang, W.S., & Akkol, E.K. (2020). Neuroprotective effects of quercetin in Alzheimer’s disease. Biomolecules, 10(1), 59.
Khosravi-Boroujeni, H., Mohammadifard, N., Sarrafzadegan, N., Sajjadi, F., Maghroun, M., Khosravi, A., et al. (2012). Potato consumption and cardiovascular disease risk factors among Iranian population. Int J Food Sci Nutr, 63(8), 913- 20.
Khosravi-Boroujeni, H., Sarrafzadegan, N., Mohammadifard, N., Sajjadi, F., Maghroun, M., Asgari, S., et al. (2013). White rice consumption and CVD risk factors among Iranian population. J Health Popul Nutr, 31(2), 252-61
Kirk, A., & Kertesz, A. (1991). On drawing impairment in Alzheimer’s disease. Arch Neurol, 48, 73-77.
Luigi, T., & Guido, G. (2016). Drawing Disorders in Alzheimer's Disease and Other Forms of Dementia. National library of medicine, 21;53(1), 31-52. doi, 10.3233/JAD-160009.
Lustbader, J.W., Cirilli, M., Lin, C., Xu, H.W., Takuma, K., Wang, N., et al. (2004). ABAD directly links Aß to mitochondrial toxicity in Alzheimer's disease. Science, 304(5669), 448-52.
Lykkesfeldt, J., & Svendsen, O. (2007 ). Oxidants and antioxidants in disease, oxidative stress in farm animals. The Veterinary Journal, 31;173(3), 502-11.
Martini, L., Melcangi, R.C., & Maggi, R. (1993). Androgen and progesterone metabolism in the central and peripheral nervous system. J Steroid Biochem Mol Biol, 47, 195-205.
McDonald, S., & Prenzler, P.D. (2001). Autolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73, 73-84.
Merzouk, H., Madani, S., Chabane Sari, D., Prost, J., Bouchenak, M., & Belleville, J. (2000). Time course of changes in serum glucose, insulin, lipids and tissue lipase activities in macrosomic offspring of rats with streptozotocin-induced diabetes. Clin Sci, 98(1), 21-30.
Monica, R., & Monica, P. (2016). The clock drawing test as a screening tool in mild cognitive impairment and very mild dementia, a new brief method of scoring and normative data in the elderly. Noural Sci, 37(6), 867-73. doi, 10.1007/s10072-016-2480-6.
Moradi, M.T., Rafieian-Koupaei, M., Imani-Rastabi, R., Nasiri, J., Shahrani, M., Rabiei, Z., et al. (2013). Antispasmodic effects of yarrow (Achillea millefolium L.) extract in the isolated ileum of rat. Afr J Tradit Complement Altern Med, 10(6), 499-503.
Mu, Y., & Gage, F.H. (2011). Adult hippocampal neurogenesis and its role in Alzheimer's disease. Mol Neurodegener, 6(1), 1-9.
Navidi, A., & Abbasi Maleki, S. (2024). Involvement of the Dopaminergic and Serotonergic Systems in the Antidepressant-like Effect Caused by Ethanolic Extract of Pistacia lentiscus in Tail Suspension Test. Arch Neurosci, 11(1), e140852. https, //doi.org/10.5812/ans-140852.
Nasri, H., & Rafieian-Kopaei, M. (2013). Oxidative stress and aging prevention. Int J Prev Med, 4(9), 1101-2.
Nasri, H., & Rafieian-Kopaei, M. (2014). Protective effects of herbal antioxidants on diabetic kidney disease. J Res Med Sci, 19(1), 82-3.
Parsaei, P., Karimi, M., Asadi, S.Y., & Rafieian-kopaei, M. (2013). Bioactive components and preventive effect of green tea (Camellia sinensis) extract on post-laparotomy intra-abdominal adhesion in rats. Int J Surg, 11(9), 811 5.
Parizad, M., & Abbasi Maleki, S. (2023). Potential Antidepressant-Like Activity of Rhus coriaria L. (Sumac) Ethanolic Extract, The Mechanism of Action via the Monoaminergic System in a Mouse Model. Jundishapur J Nat Pharm Prod, 18(3), e139330. https, //doi.org/10.5812/jjnpp-139330
Pereira, R.P., Fachinetto, R., de Souza Prestes, A., Puntel, R.L., Santos da Silva, G.N., Heinzmann, B.M., et al. (2009). Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochem Res, 34(5), 973- 83
Price, B.H., Gurvit, H., Weintraub, S., et al. (1993). Neuropsychological patterns and language deficits in20 consecutive cases of autopsy-confirmed Alzheimer’s disease. Arch Neurol, 50, 931-937.
Rabiei, Z., Bigdeli, M.R., Mohagheghi, F., & Rasolian, B. (2012). Relationship between dietary virgin Olive oil on brain Cholesterol, Cholesteryl ester and Triglyceride levels and Blood Brain Barrier (BBB) permeability in a rat stroke model. Physiol Pharmacol, 16(3), 245-54. [In Persian]
Rabiei, Z., Bigdeli, M.R., Mohagheghi, F., Rasoulian, B., & Sharifi, A. (2013). Effect of dietary olive leaf extract on brain cholesterol, cholesterol ester and triglyceride levels and of brain edema in rat stroke model. Razi J Med Sci, 19(103), 18-25. [In Persian]
Rabiei, Z., Bigdeli, M.R., Rasoulian, B., Mohagheghi, F., & Sharifi, A. (2012). The effect of various doses of olive leaf extract on brain lipid levels and blood brain barrier permeability in rat stroke model. Pajoohandeh, 17(2), 67 72. [In Persian]
Rabiei, Z., Bigdeli, M.R., & Rasoulian, B. (2013). Neuroprotection of dietary virgin olive oil on brain lipidomics during stroke. Curr Neurovasc Res, 10(3), 231-7.
Rabiei, Z., Rafieian-kopaei, M., Heidarian, E., Saghaei, E., & Mokhtari, S. (2014). Effects of Zizyphus jujube extract on memory and learning impairment induced by bilateral electric lesions of the nucleus Basalis of Meynert in rat. Neurochem Res, 39(2), 353-60.
Rabiei, Z., Rafieian-Kopaei, M., Mokhtari, S., Alibabaei, Z., & Shahrani, M. (2014). The effect of pretreatment with different doses of Lavandula officinalis ethanolic extract on memory, learning and nociception. Biomed Aging Pathol, 4(1), 71-6.
Rafieian-Kopaei, M., Baradaran, A., & Rafieian, M. (2013). Plants antioxidants, From laboratory to clinic. J Nephropathol, 2(2), 152-3.
Ranpariya, V.L., Parmar, S.K., Sheth, N.R., & Chandrashekhar, V.M. (2011). Neuroprotective activity of Matricaria recutita against fluoride- induced stress in rats. Pharm Biol, 49(7), 696-701.
Schneider, J.A., Arvanitakis, Z., Leurgans, S.E., & Bennett, D.A. (2009). The neuropathology of probable Alzheimer disease and mild cognitive impairment. Ann Neurol, 66(2), 200-8.
Sedighi, M., Nasri, H., Rafieian-kopaei, M., & Mortazaei, S. (2013). Reversal effect of Achillea millefolium extract on ileum contractions. J Herb Med Pharmacol, 2(1), 5-8.
Sharafati Chaleshtori, R., Sharafati Chaleshtori, F., & Rafieian-kopaei, M. (2011). Biological characterization of Iranian walnut (Juglans regia) leaves. Turk J Biol, 35(5), 635-9.
Sharifzadeh, M., Sharifzadeh, K., Naghdi, N., Ghahremani, M.H., & Roghani, A. (2005). Posttraining intrahippocampal infusion of a protein kinase AII inhibitor impairs spatial memory retention in rats. J Neurosci Res, 79(3), 392-400.
Shirzad, H., Shahrani, M., & Rafieian-Kopaei, M. (2009). Comparison of morphine and tramadol effects on phagocytic activity of mice peritoneal phagocytes in vivo. Int Immunopharmacol, 9(7-8), 968-70.
Shoham, S., Bejar, C., Kovalev, E., Schorer-Apelbaum, D., & Weinstock, M. (2007). Ladostigil prevents gliosis, oxidative-nitrative stress and memory deficits induced by intracerebroventricular injection of streptozotocin in rats. Neuropharmacology, 52, 836-843.
Shoham, S., Bejar, C., Kovalev, E., & Weinstock, M. (2003). Intracerebroventricular injection of streptozotocin causes neurotoxicity to myelin that contributes to spatial memory deficits in rats. Exp Neurol, 184, 1043-1052.
Szkudelski, T. (2001). The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res, 50, 336-346.
Szkudelski, T. (2001). The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res, 50, 336-346.
Villemagne, V.L., Burnham, S., Bourgeat, P., Brown, B., Ellis, K.A., Salvado, O., et al. (2013). Amyloid ß deposition, neurodegeneration, and cognitive decline in sporadic Alzheimer’s disease, A prospective cohort study. Lancet Neurol, 12(4), 357-67.
Weinstock, M., & Shoham, S., (2004). Rat models of dementia based on reductions in regional glucose metabolism, cerebral blood flow and cytochrome oxidase activity. J. Neural Transm, 111, 347-366.
Youdim, K.A., Dobbie, M.S., Kuhnle, G., Proteggente, A.R., Abbott, N.J., & Rice‐Evans, C. (2003). Interaction between flavonoids and the blood–brain barrier, in vitro studies. J Neurochem, 85(1), 180-92.
Zand, R.S., Jenkins, D.J., & Diamandis, E.P. (2001). Effects of natural products and nutraceuticals on steroid hormone-regulated gene expression. Clin Chim Acta, 312(1-2), 213-219. | ||
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