
تعداد نشریات | 41 |
تعداد شمارهها | 1,161 |
تعداد مقالات | 10,014 |
تعداد مشاهده مقاله | 18,729,793 |
تعداد دریافت فایل اصل مقاله | 13,003,150 |
بررسی تنوع ژنتیکی پروانه جوانه خوار بلوط Tortrix Viridana (Lep. Tortricidae) درجنگل های بلوط زاگرس با استفاده از s rDNA28 | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 13، شماره 2 - شماره پیاپی 44، دی 1402، صفحه 31-33 اصل مقاله (778.11 K) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2023.69145.1926 | ||
نویسندگان | ||
رضوان موسیوند1؛ محمد مجدی2؛ فواد فاتحی (همکار پیام نور)* 3؛ حامد غباری4 | ||
1گروه ژنتیک و تولید گیاهی، دانشگاه کردستان، سنندج، ایران. | ||
2دانشگاه کردستان گروه ژنتیک و تولید گیاهی، دانشگاه کردستان، سنندج، ایران | ||
3پیام نورگروه کشاورزی، دانشگاه پیام نور، تهران، ایران | ||
4گروه گیاهپزشکی، دانشگاه کردستان، سنندج، ایران | ||
چکیده | ||
پروانه جوانه خوار بلوط Tortrix viridana (Lep. Tortricidae)، یکی از آفات مهم جنگلهای زاگرس است. به منظور کنترل شیوع این آفت و جلوگیری از بروز خسارت به درختان بلوط زاگرس که از لحاظ اقتصادی دارای اهمیت ویژهای هستند، بررسی تنوع ژنتیکی جمعیتهای پروانه جوانه خوار بلوط در جنگلهای بلوط شمال غرب ایران، انجام شد. نمونههای مورد بررسی T. viridana از گونه میزبان بلوط Quercus branti و از مناطق جنگلی استانهای آذربایجان غربی، لرستان، کردستان و کرمانشاه جمع آوری شدند. به منظور بررسی تنوع ژنتیکی از ژن rDNA 28s هستهای با طول bp 850 استفاده شد. نمونهبرداری از قسمت سر و تنه حشره کامل انجام شد. استخراج DNA به روشCTAB انجام گرفت و سپس توالییابی قطعه ژنی هستهای28S rRNA انجام شد. توالیهای DNA با استفاده از نرم افزار Bioedit ویرایش شدند و همردیفی با نرم افزار MegaX و درخت فیلوژنتیک به روش neighbor-joining با تکرار 1000 و با نرم افزار MegaX ترسیم شد. نتایج درخت فیلوژنتیک نشان داد که نمونههای مختلف پروانه جوانه خوار بلوط بر اساس فاصله جغرافیایی دارای تنوع ژنتیکی هستند. ارزیابی ساختار ژنتیکی جمعیتها و برسی شاخصهای Fst و Nm نشان داد که به دلیل جریان ژنی کم مابین جمعیتها، تنوع ژنتیکی بین جمعیتها بیشتر از درون جمعیتها است. همچنین مارکر 28s چون جزء مارکرهای حفاظت شده میباشد توانسته به خوبی بین جمعیتها تفرق ایجاد کند و تنوع ژنتیکی را به درستی نشان دهد. نتایج این تحقیق میتواند در بهکارگیری روشهای مورد استفاده شیمیایی و بیولوژیک جهت کنترل این آفت مورد استفاده قرار گیرد. | ||
کلیدواژهها | ||
آفت؛ فاصله جغرافیایی؛ ژن 28s؛ Quercus brantia | ||
موضوعات | ||
ژنومیکس | ||
عنوان مقاله [English] | ||
genetic diversity of the green oak leaf roller moth, Tortrix viridana (Lep., Tortricidae) in Zagros oak forests using s rDNA28 | ||
نویسندگان [English] | ||
rezvan mousivand1؛ Mohammad Majdi2؛ ّFoad Fatehi3؛ Hamed ghobari4 | ||
1Department of Plant Production and Genetics, University of Kurdistan, Sanandaj Iran. | ||
2University of KurDepartment of plant production and Genetics, University of Kurdistan, Sanandaj Irandistan | ||
3Department of Agriculture, Payame Noor University, Tehran, Iran | ||
4Department of plant Protection, University of Kurdistan, Sanandaj Iran | ||
چکیده [English] | ||
the genetic diversity of the T. viridana on the host oak species Quercus branti was investigated using the 28s gene sequence in some Zagros forests. The samples were collected from the forest area of West Azarbaijan, Lorestan, Kurdistan and Kermanshah provinces. The Collected samples, which were in the larval stage, were grown in laboratory conditions until they turned into paup and then into complete insects, for DNA extraction. DNA extraction was done by CTAB method. 21 samples were selected to investigate genetic diversity using the 28s gene, and 18 sequences DNAs were of suitable quality for further investigations. In order to amplify 28s region, based on the sequence obtained from the NCBI site.The desired region in the collected samples was amplified using the PCR method and the resulting product was sequenced. The sequences were edited using Bioedit software and aligned with the help of MegaX software, and the phylogenetic tree was drawn by UPGMA method with 1000 sampling repetitions. The results of this study, based on the phylogenetic tree, showed that the different samples of the oak saplings have genetic diversity based on the geographical distance. Assessment of genetic structure of the populations showed that higher diversity was demonstrated in intrapopulations than interpopulations geographically. 28s gene showed the presence of 5 haplotypes in these populations. The presence of low diversity between populations indicated the existence of gene flow between populations as well as genetic compatibility in the studied populations. | ||
کلیدواژهها [English] | ||
Pest, geographical distance, gene 28s, Quercus branti | ||
سایر فایل های مرتبط با مقاله
|
||
مراجع | ||
Zargaran, M. Mousavi Mirkla, S.R. Banj Shafi'i, A.
and Ramezani Kakroudi, A. (2014). Studying the
biology of the oak borer and its distribution in
West Azarbaijan province. Journal of Forest
Research and Development, 1 (1), 31-42.
Talebi, Kh. Sajdi, T., & Yazdian, F. (2014). A look
at the forests of Iran. The second edition,
published by the Forestry and Rangeland
Research Institute. Pages 1-65.
Ghabari, H. Guldansaz, S.H. Ashuri, A., Kharazi,
A., & Bi-Hamta, M. (2016). Investigating the
presence and distribution of the oak bud-eating
moth in the forests of Kurdistan province.
Entomological Society letter. 27 (1), 47-59.
Ghiyasuddin, H., Shabanian, N., Kavossi, M., &
Talebi, R. (2019). Genetic diversity and
population structure of the oak bud-eating
butterfly in the forests of North Zagros. Journal
of modern genetics. 15 (2), 136-123.
Assefa, Y., Goftishu, M., Capdevielle-Dulac, C., &
Le Ru, B. (2017). Clarifying the source of
Conicofrontia sesamoides Hampson
(Lepidoptera: Noctuidae) population in South
African sugarcane using morphological
identification and mitochondrial DNA analysis.
Phytoparasitica, 45(1), 45-55.
Aylor, D.E., & Irwin, M.E. (1999). Aerial dispersal
of pests and pathogens: implications for
integrated pest management. Agricultural and
Forest Meteorology, 97, 233-234.
Bhau, B.S., Mech, J., Borthakur, S., Bhuyan, M., &
Bhattacharyya, P.R. (2014). Morphological and
genetic diversity studies among populations of
tea mosquito bug, Helopeltis theivora from
Assam, India. Molecular Biology Reports, 41,
7845-7856.
Castagneyrol, B., Jactel, H., Vacher, C., Brockerhoff,
E.G., & Koricheva, J. (2014). Effects of plant
phylogenetic diversity on herbivory depend on
herbivore specialization. Journal of Applied
Ecology, 51(1), 134-141.
Du Merle, P. (1999). Egg development and
diapause: ecophysiological and genetic basis of
phenological polymorphism and adaptation to
varied hosts in the green oak tortrix, Tortrix
viridana L. (Lepidoptera: Tortricidae). Journal
of Insect Physiology, 45(6), 599-611.
Fazeli, M.J., & Abai, M. (1990). Green oak leaf-roller
moth in Kohkiluyeh and Boyer-Ahmad province
(Tortrix viridana L., Lep.: Tortricidae). Applied
Entomology and Phytopathology, 57(1-2), 1-2.
Gao, B., Hedlund, J., Reynolds, D. R., Zhai, B., Hu,
G., & Chapman, J. W. (2020). The ‘migratory
connectivity’concept, and its applicability to
insect migrants. Movement Ecology, 8, 1-13.
Ghobari, H., Goldansaz, S.H., Askari, H., Ashouri,
A., kharazi, P.A., & Bihamta, M.R. (2007).
Investigation of presence, distribution and flight
period of oak leaf roller moth, Tortrix viridana
(Lep.: Tortricidae) using pheromone traps
inKurdistan province. Journal of Entomological
Society of Iran, 27(1), 47-59.
Guo, J., Wang, Z., & Francis, F. (2017). Use of
molecular markers for entomological diversity
assessment and their application in population
study of aphids. Entomologie faunistiqueFaunistic Entomology.
Haase, J., Castagneyrol, B., Cornelissen, J.H.C.,
Ghazoul, J., Kattge, J., Koricheva, J., Scherer
Lorenzen, M., Morath, S., & Jactel, H. (2015).
Contrasting effects of tree diversity on young
tree growth and resistance to insect herbivores
across three biodiversity experiments. Oikos,
124(12), 1674-1685.
Hunter, M.D. (2008). A variable insect–plant
interaction: the relationship between tree
budburst phenology and population levels of
insect herbivores among trees. Journal of
Ecological Entomology, 17(1), 91-95.
Jazirehi, M.H., & Ebrahimi-Rastaghi, M. (2003).
Silviculture in Zagros. Tehran University
publications. (In Farsi).
Jones, B.C., & Despland, E. (2006). Effects of
synchronization with host plant phenology occur
early in the larval development of a spring folivore.
Canadian Journal of Zoology, 84(4), 628-633.
Li, X., Wu, S., Xu, Y., Liu, Y., & Wang, J. (2022).
Population Genetic Structure of Chlorops oryzae
(Diptera, Chloropidae) in China. Insects, 13(4),
327.
Marvi mohajer, MR. (2005). Silviculture, Tehran
University Prees, Tehran, 388p. (In Pertion).
Nugnes, F., Gebiola, M., Monti, M.M., Gualtieri,
L., Giorgini, M., Wang, J., & Bernardo, U.
(2015). Genetic Diversity of the Invasive Gall
Wasp Leptocybe invasa (Hymenoptera:
Eulophidae) and of its Rickettsia Endosymbiont,
and Associated Sex-Ratio Differences. Journal
of Plos one, 10(5), 1-20.
Sabeti, H.A. (1995). Forests, trees and shrubs of
Iran. Yaz University publications. (In Farsi).
Salvato, P., Battisti, A., Concato, S., Masutti, L.,
Patarnello, T., & Zane, L. (2002). Genetic
differentiation in the winter pine processionary
moth (Thaumetopoea pityocampa–wilkinsoni
complex), inferred by AFLP and mitochondrial
DNA markers. Mol Ecol 11, 2435-2444.
44 موسیوند و همکاران: بررسی تنوع ژنتیکی پروانه جوانه خوار بلوط (Tortricidae .,Lep (.L viridana Tortrix ...
Schroder, H. (2008). Genetic differentiation of
populations of the green oak leaf roller (Tortrix
viridana L.) and its host (Quercus robur L.)
using nuclear gene markers. Mitteilungen der
Deutschen Gesellschaft fur Allgemeine und
Angewandte Entomologie, 16(4), 237-242.
Schroeder, H., & Degen, B. (2008a). Genetic structure
of the green oak leaf roller (Tortrix viridana L.)
and one of its hosts, Quercus robur L. Forest
Ecology and Management, 256, 1270-1279.
Schroeder, H., & Degen, B. (2008b). Spatial genetic
structure in populations of the green oak leaf
roller, Tortrix viridana L. (Lepidoptera,
Tortricidae). European Journal of Forest
Research, 127(6), 447-453.
Schroeder, H., Arens, P., & Smulders, M.J.M.
(2010). Autosomal and sex-linked microsatellite
loci in the green oak leaf roller Tortrix viridana
L. (Lepidoptera, Tortricidae). Journal of
Molecular Ecology Resources, 9(3), 809-811.
Serra, G., Maestrale, GB., Baratti, M., & Lentini, A.
(2014). Genetic variation in Sardinian populations
of the Green oak leaf roller Tortrix viridana L.
(Lepidoptera, Tortricidae). Integrated protection in
oak forests. IOBC/wprs Bulletin, 101, 221-225.
Shuster, S.M., Lonsdorf, E.V., Wimp, G.M., Bailey,
J.K., & Whitham, T.G. (2006). Community
heritability measures the evolutionary
consequences of indirect genetic effects on
community structure. Evolution, 60(5), 991-1003.
Wang, X., & Messing, R.H. (2003). Intra- and
interspecific competition by Fopius arisanus and
Diachasmimorpha tryoni (hymenoptera:
Braconidae), parasitoids of tephritid fruit flies.
Biological Control, 27, 251-259.
Wharton, R.A., Trostle, M.K., Messing, R.H.,
Copeland, R.S., Kimani-Njogu, S.W., Lux, S.,
Overholt, W.A., Mohamed, S., & Sivinski, J.
(2000). Parasitoids of medfly, ceratitis capitata,
and related tephritids in kenyan coffee: a
predominantly koinobiont assemblage. Bulletin
of Entomological Research, 90, 517-526.
Xu, Y., Mai, J.W., Yu, B.J., Hu, H.X., Yuan, L.,
Jashenko, R., & Ji, R. (2019). Study on the
genetic differentiation of geographic populations
of Calliptamus italicus (Orthoptera: Acrididae)
in sino-kazakh border areas based on
mitochondrial COI and COII genes. Journal of
Economic Entomology, 112(4), 1912-1919. | ||
آمار تعداد مشاهده مقاله: 82 تعداد دریافت فایل اصل مقاله: 181 |