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تجزیه بیان دو ژن گیرنده شبهکینازی در گیاهچه های جو تحت تیمار سالیسیلیکاسید و شوری | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 1، دوره 6، شماره 1 - شماره پیاپی 15، آذر 1395، صفحه 1-11 اصل مقاله (319.69 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| خدیجه نوروزی نوروزی1؛ رضا فتوت* 2؛ فرید شکاری3؛ احسان محسنی فرد4 | ||
| 1دانشجوی سابق کارشناسیارشد بیوتکنولوژی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان | ||
| 2استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان | ||
| 3دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان | ||
| 4استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان | ||
| چکیده | ||
| شوریخاک از مهمترین تنش های غیرزیستی است که شدیداً تولید محصول را کاهش می دهد. مطالعات اخیر نشان داده است که کاربرد بیرونی سالیسیلیکاسید بهعنوان یک ملکول مهم در مسیر ترارسانی در انتقال پیام های تنش های غیرزیستی نقش دارد. با وجود این نقش آن در ترارسانی تنش شوری روشن نیست. در این تحقیق اثر غلظت های مختلف سالیسیلیکاسید، در بیان ژن های گیرنده ی شبهکینازی دو رقم جو (ریحان و نصرت) متفاوت از لحاظ مقاومت به شوری مورد بررسی قرار گرفت. نتایج نشان داد که شوری و سالیسیلیکاسید تأثیر معنی داری بر صفات گیاهچهای جو داشتهاند و موجب افزایش بیان نسبی ژن های Hv3ARK و IdiRLK2 در رقم نصرت شدند. ولی در رقم ریحان بیان نسبی ژن ها متفاوت بود. به نظر می رسد سالیسیلیکاسید با فعال کردن گیرندههای شبهکینازی در مسیر انتقال پیام شوری قرار دارد. این نتایج نشان می دهد که برخی از گیرنده های شبهکینازی، ممکن است در پاسخهای بواسطه سالیسیلیکاسید در گیاهان تحت تنش های غیرزیستی نقش داشته باشند. | ||
| کلیدواژهها | ||
| Hordeum vulgare؛ پروتئینهای غشائی؛ کینازهای پروتئینی؛ ترارسانی | ||
| موضوعات | ||
| بیوتکنولوژی و تنش های زنده و غیرزنده | ||
| عنوان مقاله [English] | ||
| Expression analysis of two receptor-like kinase genes in barley seedling under salt and salicylic acid treatment | ||
| نویسندگان [English] | ||
| Khadijeh Noroozi1؛ Reza Fotovat2؛ Farid Shekari3؛ Ehsan Mohsenifard4 | ||
| 1Former Student of Plant Biotechnology, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran | ||
| 2Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran. | ||
| 3Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran. | ||
| 4Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran. | ||
| چکیده [English] | ||
| Soil salinity is one of the major abiotic stresses that dramatically reducing crop productivity in the world. Recent studies indicate that the application of exogenous salicylic acid (SA) as a key molecule in the signal transduction pathway participates in the signaling of abiotic stresses. The role of salicylic acid in salinity signalling is, however, unclear. In the present study, two barley (Hordeum vulgare L.) varieties (Reyhan and Nosrat) contrasting in their salinity tolerance were used to assess the role of various SA concentrations on the expression of Receptor-like kinases (RLKs) genes. The results demonstrated that that salinity and salicylic have a significant effect on seedling traits of barley cultivars. Salinity stress and salicylic acid increased the expression of Hv3ARK, IdiRLK2 genes in the cultivar Nosrat but in the other cultivar the relative expression of the genes were different. These results suggest the involvement of a number of RLKs in SA-mediated abiotic stress responses. | ||
| کلیدواژهها [English] | ||
| Hordeum vulgare, membrane protein, protein kinase, signal transduction | ||
| مراجع | ||
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