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Selective Determination of Glucose in Blood Plasma by Using an Amperometric Glucose Biosensor Based on Glucose Oxidase and a Chitosan/ Nafion/ IL/Ferrocene Composite Film | ||
| Iranian Journal of Analytical Chemistry | ||
| مقاله 2، دوره 5، شماره 1، خرداد 2018، صفحه 9-16 اصل مقاله (1.38 M) | ||
| نوع مقاله: Full research article | ||
| نویسندگان | ||
| Arezoo Zohourtalab* ؛ Habib Razmi | ||
| Electroanalytical Chemistry Research Lab., Faculty of Sciences, Azerbaijan Shahid Madani University, P.O. Box: 53714-161, Tabriz, Iran | ||
| چکیده | ||
| A kind of ferrocene mediated enzymatic biosensor was fabricated in order to the measurement of glucose as an important biological analyte. A homogenous mixture of ferrocene including Chitosan(CS), Nafion(Nf) and an imidazolium based ionic liquid, 1-butyl-3-methyl imidazoliumhexafluorophosphate, [BMIM]HF6PO4, was prepared. The mentioned mixture was cast on the surface of carbon ceramic electrode and an enzymatic layer was entrapped between two layers of this mixture in order to improving the mechanical stability of the biosensor. Cyclic voltammetry and chronoamperometry were used to investigate the analytical performance of the biosensor. Remarkable deduction of interfering signals produced by AA and UA was obtained by applying a constant potential (180mV) on the human plasma for a short period of time (100min). Values of practical parameters such as sensitivity (2.73 µA mM-1cm-2), linear range (95.23–1367.5 µM) and detection limit (48 µM) were obtained by the chronoamperometric studies. The apparent Michaels-Menten constant, Km (3.52 mM) was also calculated. In order to estimate the practical accuracy and precision of the enzymatic biosensor, a test of spiked glucose solution recovery in natural plasma was done. | ||
| کلیدواژهها | ||
| Glucose Oxidase؛ Carbon Ceramic Electrode؛ Biosensor؛ ionic liquid؛ Chitosan؛ Nafion | ||
| عنوان مقاله [English] | ||
| تعیین گزینشی گلوکز در پلاسمای خون با استفاده از حسگر زیستی آمپرومتری گلوکز مبتنی بر آنزیم گلوکز اکسیداز و فیلم پیچیدهی متشکل از کیتوسان/ نافیون/ مایع یونی/ فروسن | ||
| نویسندگان [English] | ||
| آرزو ظهورطلب؛ حبیب رزمی | ||
| آزمایشگاه تحقیقاتی الکتروشیمیایی، دانشکده علوم، دانشگاه شهید مدنی آذربایجان، صندوق پستی 161-53714، تبریز، ایران | ||
| چکیده [English] | ||
| نوعی از حسگر زیستی آنزیمی مبتنی بر وساطت فروسن به منظور اندازه گیری گلوکز به عنوان یک آنالیت مهم زیستی تهیه شده است. مخلوط همگنی از فروسن شامل کیتوسان (CS)، نافیون (Nf) و یک مایع یونی مبتنی بر ایمیدازولیوم 1- بوتیل- 3 متیل ایمیدازولیوم هگزا فلوئورو فسفات [BMIM]HF6PO4، تهیه گردید. مخلوط مذکور بر سطح الکترود کربن سرامیک قرار داده شد و به منظور پایداری مکانیکی حسگر زیستی لایه ای از آنزیم بین دو لایه از این مخلوط به دام انداخته شد. برای بررسی عملکرد تجزیه ای حسگر زیستی از ولتامتری چرخه ای و کرونو آمپرومتری استفاده شد. با اعمال پتانسیل ثابتی (180 میلی ولت) بر پلاسمای انسانی برای مدت زمان کوتاهی (100 دقیقه)، کاهش قابل ملاحظه ای در علایم مزاحم ناشی از آسکوربیک اسید (AA) و اوریک اسید (UA) ایجاد شد. مقادیر پارامتر های عملی مانند حساسیت (µA mM-1cm-22.73)، محدوده ی خطی (µM 1367.5-95.23) و حد تشخیص (µM48) با مطالعات کرونو آمپرومتری به دست آمدند. هم چنین ثابت ظاهری مایکلیس منتن Km (mM 3.52)، محاسبه شد. برای تخمین صحت و دقت عملی حسگر زیستی، آزمایش بازیابی گلوکز وارد شده در پلاسمای طبیعی انجام شد. | ||
| کلیدواژهها [English] | ||
| گلوکز اکسیداز, اکترود کربن سرامیک, حسگر زیستی, مایع یونی, کیتوسان, نافیون | ||
| مراجع | ||
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