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غنی‌سازی مواد غذایی جلبک کلرلا Chlorella sorokiniana (Shihira & R.W. Krauss) | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 11، دوره 7، شماره 2 - شماره پیاپی 26، آذر 1397، صفحه 105-114 اصل مقاله (465.59 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2018.5186 | ||
| نویسندگان | ||
| آمنه جمشیدی* 1؛ محمدعلی ابراهیمی2؛ طیبه رجبیان3؛ غلامرضا بخشی حانیکی4؛ شهلا مظفری5 | ||
| 1مربی گروه زیست‌شناسی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران. | ||
| 2دانشیار گروه بیوتکنولوژی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران. | ||
| 3دانشیار گروه زیست‌شناسی، دانشگاه شاهد، صندوق پستی 3319118651، تهران، ایران. | ||
| 4استاد گروه کشاورزی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران. | ||
| 5استادیار گروه شیمی، دانشگاه پیام نور، صندوق پستی 3697-19395، تهران، ایران. | ||
| چکیده | ||
| چکیده جلبک کلرلا (Chlorella sorokiniana) حاوی متابولیت‌های ارزشمندی مانند پروتئین‌ها، ترکیبات آنتی‌اکسیدان، لیپیدها، ویتامین‌ها و مواد معدنی است و بهعنوان غذای جانوران ودارو مورد استفاده قرار میگیرد. به همین دلیل در پژوهش حاضر، اثر تغییر شرایط کشت، جهت تولید مکمل غذایی مغذیتر از جلبک برای تغذیه آبزیان بررسی شد. بدین منظور، جلبکها در محیط کشت پایه بولد اصلاح‌شده Bold (BBM) کشت شد و بهعنوان منبع کربن به آن5 گرم در لیتر گلوکز افزوده شد. سپس اثر pH، افزودن تیامین پیروفسفات، تغییر مقدار نیترات و فسفات، افزودن مخمر و فقدان گلوکز، بر روی رشد و محتویات متابولیت‌های جلبک مورد بررسی قرار گرفت. بیشترین میزان افزایش رشد، محتوای آنتیاکسیدانها، پروتئین وبرخی موادمعدنی در کشت‌های جلبکی، در محیطهای کشت حاوی غلظت دو برابر نیترات و فسفات واجد تیامین پیروفسفات و بدون تیامین پیروفسفات بهدست آمد. افزودن مخمر به شکل غیرمعنی‌داری سبب افزایش وزن خشک و کاهش آنتیاکسیدانها شد. افزودن تیامین پیروفسفات به تنهایی و کاهش اندک اسیدیته اثر چندانی بر رشد و محتوای متابولیت‌ها در جلبک مورد مطالعه نداشت. فقدان گلوکز سبب کاهش چشمگیر رشد جلبک شد. | ||
| کلیدواژه‌ها | ||
| واژه‌های کلیدی: Chlorella sorokiniana؛ نیترات؛ فسفات؛ آنتی اکسیدان؛ پروتئین | ||
| عنوان مقاله [English] | ||
| Food enrichment of Chlorella sorokiniana (Shihira & R.W. Krauss) | ||
| نویسندگان [English] | ||
| Ameneh Jamshidi1؛ Mohammad Ali Ebrahimi2؛ Tayebeh Rajabian3؛ Gholam Reza Bakhshikhaniki4؛ Shahla Mozaffari5 | ||
| 1Instructor, Department of Biology, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran. | ||
| 2Assiocate Professor, Department of Agriculture, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran. | ||
| 3Assiocate Professor, Department of Biology, Faculty of Basic Scienses, Shahed University, P.O. Box 331911865, Tehran, Iran. | ||
| 4Professor, Department of Agriculture, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran. | ||
| 5Assistant Professor, Department of Chemistry, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran. | ||
| چکیده [English] | ||
| Abstract Chlorella sorokiniana contains valuable metabolites such as proteins, antioxidants, lipids, vitamins and minerals, and is used as a food for animals and medicine. Therefore, in the present study, the effect of some culture factors and conditions on nutrient supplementation of algal levels for aquaculture was investigated. For this purpose, algae were cultured in modified Bold Basal Media (BBM) and 0.5 gram of glucose was added as carbon source. Then, the effect of pH, addition of thiamine pyrophosphate, changes in nitrate and phosphate levels, addition of yeast and lack of glucose, were studied on growth and content of algal metabolites. The highest growth rate, antioxidant content, protein and some algal mineral content were obtained in medium containing twice the nitrate and phosphate with or without thiamine pyrophosphate. Adding yeast increased dry weight and decreased none significantly antioxidants. Adding thiamine pyrophosphate alone and low reduction of acidity did not significantly effect on growth and metabolites of alga. Lack of glucose significantly reduced alga growth. | ||
| کلیدواژه‌ها [English] | ||
| Keywords: Chlorella sorokiniana, nitrate, phosphate, antioxidant, protein | ||
| مراجع | ||
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