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Analytical Methods for the Determination of Amikacin in Pharmaceutical Preparations and Biological Fluids: A Review | ||
Iranian Journal of Analytical Chemistry | ||
مقاله 6، دوره 5، شماره 2، آذر 2018، صفحه 39-55 اصل مقاله (831.83 K) | ||
نوع مقاله: Full research article | ||
نویسندگان | ||
Muneeba Usmani؛ Sofia Ahmed؛ Muhammad Sheraz* ؛ Iqbal Ahmad | ||
Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, 51, Deh Tor, Super Highway, Gadap Road, Karachi, Pakistan | ||
چکیده | ||
Amikacin (AM) belongs to the family of aminoglycoside antibiotics. It is a broad spectrum antibiotic, effective against both Gram-negative and Gram-positive bacterial infections. The chemical structure of AM consists of hydroxyl (OH), glucopyranosyl and amino (NH2) groups. However, the lack of chromophore in the AM structure made it difficult to detect it in the UV-visible region (200–800 nm). Therefore, derivatization of AM is carried out to introduce a chromophore in its structure which helps in its detection in the UV-visible region. Several methods have been developed and reported for the determination of AM in pharmaceutical and biological samples. These methods include chromatographic techniques such as thin layer chromatography (TLC), liquid chromatography (LC), hydrophilic interaction liquid chromatography (HILC), high performance liquid chromatography (HPLC), ultra-high performance liquid chromatography (UHPLC), ion chromatography (IC); spectrometry such as UV-visible spectrometry, spectrofluorimetry, nuclear magnetic resonance (NMR) spectrometry, mass spectrometry (MS); chemiluminescence; Rayleigh scattering method; electrochemical methods such as polarography, cyclic voltammetry, amperometry; immunoassay and microbial assay. This review highlights the application of these methods in the analysis of AM in pharmaceutical and biological samples. | ||
کلیدواژهها | ||
Amikacin؛ Analysis؛ Chromatography؛ Spectrometry؛ Electrochemical Methods؛ Immunoassay | ||
عنوان مقاله [English] | ||
روشهای تجزیهای برای تعیین آمیکاسین در نمونههای دارویی و سیالات بیولوژیکی: مقاله مروری | ||
نویسندگان [English] | ||
منیب عثمان؛ سوفیا احمد؛ محمدعلی شراز؛ اقبال احمد | ||
موسسه علوم دارویی باکی، دانشگاه پزشکی باکی، بزرگراه سوپر، جاده گاتاپ، کراچی، پاکستان | ||
چکیده [English] | ||
چکیده: آمیکاسین متعلق به خانوادهی آمینوگلیکوزیدها از دسته آنتیبیوتیکها میباشد. آمیکاسین طیف وسیعی از آنتیبیوتیکهاست که در برابر عفونتهای باکتریایی گرم مثبت و گرم منفی موثر میباشد. ساختمان شیمیایی آمیکاسین شامل گروههای هیدروکسیل، گلیکو پیرانوزیل و آمینی میباشد. به هر حال فقدان کروموفر در ساختار آمیکاسین، شناسایی آن را در ناحیه ماوراء بنفش–مرئی ( 200 تا 800 نانومتر) مشکل میسازد. بنابراین، مشتقسازی از آمیکاسین صورت گرفت تا یک گروه کروموفر در ساختار آن وارد گشته و به شناسایی آن در ناحیه ماوراء بنفش-مرئی کمک کند. روشهای متعددی برای تعیین آمیکاسین در نمونههای بیولوژیکی و دارویی توسعه و گزارش شده است. این روشها شامل تکنیکهای کروماتو گرافی از جمله کروماتوگرافی لایه نازک، کروماتوگرافی مایع، کروماتوگرافی مایع برهمکنشی آبدوستی، کروماتوگرافی مایع با کارآیی بالا، کروماتوگرافی مایع با کارآیی فوقالعاده بالا، کروماتوگرافی یونی، طیفسنجی ناحیه مرئی-ماوراء بنفش، اسپکتروفلوئوریمتری، اسپکترومتری رزنانس مغناطیسی هسته، اسپکترومتری جرمی، کمی لومینسانس، روش پراکندگی رالی، روش های الکتروشیمی مانند پلاروگرافی، ولتامتری چرخهای، آمپرومتری، سنجشهای میکروبی و ایمنسنجی میباشد. این مقاله کاربرد این روشها را در تجزیه آمیکاسین در نمونههای دارویی و بیولوژیکی مشخص می نماید. | ||
کلیدواژهها [English] | ||
آمیکاسین, تجزیه, کروماتوگرافی, طیفسنجی, روشهای الکتروشیمی, ایمنسنجی | ||
مراجع | ||
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