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Shaghaghi, Masoomeh, Dehghan, Gholamreza, Khajevand, Fatemeh. (1400). Synthesis, Characterization, Antioxidant and ctDNA -Binding Activity of Bi(III)-Quercetin Complex: Multispectral Analysis. فصلنامه علمی زیست فناوری گیاهان زراعی, 9(1), 15-26. doi: 10.30473/ijac.2022.62686.1224
Masoomeh Shaghaghi; Gholamreza Dehghan; Fatemeh Khajevand. "Synthesis, Characterization, Antioxidant and ctDNA -Binding Activity of Bi(III)-Quercetin Complex: Multispectral Analysis". فصلنامه علمی زیست فناوری گیاهان زراعی, 9, 1, 1400, 15-26. doi: 10.30473/ijac.2022.62686.1224
Shaghaghi, Masoomeh, Dehghan, Gholamreza, Khajevand, Fatemeh. (1400). 'Synthesis, Characterization, Antioxidant and ctDNA -Binding Activity of Bi(III)-Quercetin Complex: Multispectral Analysis', فصلنامه علمی زیست فناوری گیاهان زراعی, 9(1), pp. 15-26. doi: 10.30473/ijac.2022.62686.1224
Shaghaghi, Masoomeh, Dehghan, Gholamreza, Khajevand, Fatemeh. Synthesis, Characterization, Antioxidant and ctDNA -Binding Activity of Bi(III)-Quercetin Complex: Multispectral Analysis. فصلنامه علمی زیست فناوری گیاهان زراعی, 1400; 9(1): 15-26. doi: 10.30473/ijac.2022.62686.1224

Synthesis, Characterization, Antioxidant and ctDNA -Binding Activity of Bi(III)-Quercetin Complex: Multispectral Analysis

Iranian Journal of Analytical Chemistry
دوره 9، شماره 1 - شماره پیاپی 17، خرداد 2022، صفحه 15-26    اصل مقاله (852.95 K)
نوع مقاله: Full research article
شناسه دیجیتال (DOI): 10.30473/ijac.2022.62686.1224
نویسندگان
Masoomeh Shaghaghi* 1؛ Gholamreza Dehghan2؛ Fatemeh Khajevand2
1Department of Chemistry, Payame Noor University, P. O. Box 19395-4697 Tehran, Iran
2Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
چکیده
Chelation therapy has been used to remove toxic metals from the body for a long time. Flavonoids such as quercetin (QUR), a well-known protective antioxidant and free radical scavenger, can bind to metal cations and protect our bodies from toxic metals. In the current study, we used UV–Vis, 1HNMR, IR, and fluorescence spectroscopic techniques as well as viscosity measurements to investigate the synthesis, characterization, and interaction between Bi(III)-QUR complex and calf thymus DNA (ctDNA) in physiological buffer. The antioxidant activity of the complex was assessed utilizing DPPH and ABTS free radical scavenging, and ferric reducing potential. After chelation of the Bi(III) cation, the antioxidant potential of QUR was reduced. In the presence of ctDNA, the absorption spectrum of Bi(III)-QUR complex was raised, and the fluorescence intensity of Bi(III)-QUR complex was increased. With the addition of the Bi(III)-QUR complex, the relative viscosity of ctDNA rose. These findings indicate that the Bi(III)-QUR complex interacts with ctDNA in a groove-binding mode. The thermodynamic parameters (ΔH, ΔS, and ΔG) of the Bi(III)-QUR complex with ctDNA, as well as well as association constant, Ka, and number of binding sites (n), were assessed from the fluorescence data, indicating that the binding of Bi(III)-QUR complex to ctDNA was primarily driven by hydrophobic interactions.
کلیدواژه‌ها
Flavonoids؛ Chelation Therapy؛ Bi(III)-Quercetin Complex؛ Radical Scavenging؛ Natural Products؛ Antioxidant
عنوان مقاله [English]
سنتز، مشخصه‌یابی و بررسی فعالیت آنتی‌اکسیدان و اتصال به DNA دو رشته کمپلکس کرستین-بیسموت (III): مطالعات اسپکتروسکوپی
نویسندگان [English]
معصومه شقاقی1؛ غلامرضا دهقان2؛ فاطمه خواجه وند2
1گروه شیمی، دانشگاه پیام نور، صندوق پستی 4697-19395 تهران، ایران
2گروه زیست‌شناسی، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران
چکیده [English]
چکیده
شلات درمانی از دیرباز برای حذف فلزات سمی از بدن مورد استفاده قرار می­گرفته است. فلاونوئیدهایی مانند کوئرستین (QUR)، یک آنتی اکسیدان محافظ شناخته شده و بعنوان پاک­کننده رادیکال­های آزاد، می­توانند به کاتیون­های فلزی متصل شده و از بدن ما در برابر فلزات سمی محافظت کنند. در مطالعه حاضر، از تکنیک‌های طیف‌سنجی ماوراء بنفش-مرئی (UV-vis)، 1HNMR، IR و فلوئورسانس و همچنین اندازه‌گیری‌های ویسکوزیته برای سنتز، مشخصه یابی و مطالعه برهم­کنش بین کمپلکس Bi(III)-QUR و DNA تیموس گوساله (ctDNA) در بافر فیزیولوژیکی استفاده شد. فعالیت آنتی اکسیدانی کمپلکس با استفاده از مهار رادیکال آزاد DPPH و ABTS و پتانسیل کاهش آهن (III) مورد ارزیابی قرار گرفت. پس از شلاسیون کاتیون بیسموت، (III)Bi، پتانسیل آنتی­اکسیدانی QUR کاهش یافت. در حضور ctDNA، طیف جذب کمپلکس Bi(III)-QUR و شدت فلوئورسانس کمپلکس Bi(III)-QUR افزایش یافت. همچنین با افزودن کمپلکس Bi(III)-QUR، ویسکوزیته نسبی ctDNA افزایش یافت. این یافته‌ها نشان می‌دهد که کمپلکس Bi (III)-QUR با ctDNA در حالت اتصال به شیار برهم کنش می­دهد. پارامترهای ترمودینامیکی (ΔH، ΔS و ΔG) کمپلکس Bi(III)-QUR با ctDNA، و همچنین ثابت اتصال، Ka، و تعداد مکان‌های اتصال (n)، از داده‌های فلوئورسانس ارزیابی شدند، و نتایج نشان داد که اتصال کمپلکس Bi(III)-QUR به ctDNA عمدتا بواسطه نیروهای آبگریز پیش می­رود.
کلیدواژه‌ها [English]
فلاونوئیدها, شلات درمانی, کمپلکس Bi(III)-QUR, پاکسازی رادیکال, محصولات طبیعی, آنتی‌اکسیدان
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