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تغییرات سطح بیان ژن BTG1 در افراد مبتلا به سرطان معده و مقایسه آن با افراد سالم | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 10، شماره 4 - شماره پیاپی 40، خرداد 1401، صفحه 35-46 اصل مقاله (1.54 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2021.58999.1829 | ||
| نویسندگان | ||
| حمید علی بهار1؛ یوسف پریدار2؛ میثم مردسلطانی3؛ رضا حاجی حسینی* 4 | ||
| 1دکتری، گروه بیوشیمی، پیام نور، تهران، ایران | ||
| 2استادیار، کلینیک ویژه بیماریهای گوارش و کبد بالغین، دانشگاه علوم پزشکی دزفول، دزفول، ایران | ||
| 3استادیار، گروه بیوشیمی بالینی، دانشکده علوم پزشکی، دانشگاه علوم پزشکی دزفول، دزفول، ایران | ||
| 4استاد، گروه بیوشیمی، پیام نور، تهران، ایران | ||
| چکیده | ||
| سرطان معده (GC) یکی از متداول ترین انواع سرطان و دومین علت اصلی مرگومیر ناشی از سرطان است. تأخیر در تشخیص و عدم غربالگری از دلایل اصلی مرگومیر بالا در اثر این بیماری است. به نظر میرسد یافتن یک نشانگر زیستی تشخیصی دقیق و مؤثر برای درمان مؤثرGC ضروری است. در این راستا با استفاده از گاستروسکوپ، ما نمونههای بافتی را از بیماران مبتلا بهGC و افراد سالم جمع آوری کردیم. از نمونههای بهدستآمده برای استخراج RNA آنها با استفاده از کیت محلولTrizol استفاده شد. از نمونههای RNA برای انجامqRT-PCR با استفاده از آغازگرهای اختصاصی طراحیشده برای ژنهایBTG1 وGAPDH استفاده شد. نتایجqRT-PCR با استفاده از روش -ΔCt2 و آزمونهای آماری مختلف از طریق نرمافزار SPSS مورد تجزیه و تحلیل قرار گرفت. در مجموع، 40 نمونه از GC و شاهد سالم جمع آوری شد و اطلاعات دموگرافیک آنها ثبت شد. استخراج RNA مقدار RNA لازم را برای qRT-PCR تولید کرده بود. نتایج qRT-PCR نشان داد، که بیانBTG1 بهطور قابلتوجهی در نمونههای GC کاهش یافته است. باتوجه به نتایج بهدستآمده میتوان نتیجه گرفت که کاهش بیانBTG1 میتواند بهعنوان یک نشانگر زیستی دقیق برایGC عمل کند. این ژن همچنین میتواند شاخص بیماریزاییGC باشد. این نتایج میتواند کاربردهای تشخیصی و درمانی احتمالیBTG1 برای GC را نشان دهد. | ||
| کلیدواژهها | ||
| تشخیص؛ سرطان معده؛ نشانگرزیستی؛ BTG1 | ||
| عنوان مقاله [English] | ||
| Changes in BTG1 Gene Expression Level in People with Gastric Cancer and Its Comparison with Healthy People | ||
| نویسندگان [English] | ||
| Hamid Alibahar1؛ Yousef Paridar2؛ Maysam Mard-soltani3؛ Reza Haji Hosseini4 | ||
| 1Ph. D., Department of Biochemistry, Payame Noor University (PNU), P.O. Box 19395-4697, Tehran, Iran | ||
| 2Assistant Professor, Gastroenterology Clinic, Dezful University of Medical Sciences, Dezful, Iran | ||
| 3Assistant Professor, Department of Clinical Biochemistry, Faculty of Medical Sciences, Dezful University of Medical Sciences, Dezful, Iran | ||
| 4Professor, Department of Biochemistry, Payame Noor University (PNU), P.O. Box 19395-4697, Tehran, Iran | ||
| چکیده [English] | ||
| Gastric cancer (GC) is one of the most common types of cancer and the second leading cause of cancer mortality. Delay in diagnosis and lack of screening are the main causes of high mortality from this disease. Finding an accurate and effective diagnostic biomarker seems to be essential for effective treatment of GC. In this regard, using a gastroscope, we collected tissue samples from patients with GC and healthy individuals. The obtained samples were used to extract their RNA using Trizol solution kit. RNA samples were used for qRT-PCR using specific primers designed for BTG1 and GAPDH genes. QRT-PCR results were analyzed using 2-ΔCt method and various statistical tests using SPSS software. In total, 40 samples of GC and healthy controls were collected and their demographic information was recorded. RNA extraction produced the amount of RNA required for qRT-PCR. The qRT-PCR results showed that BTG1 expression was significantly decreased in GC samples. According to the obtained results, it can be concluded that the reduction of BTG1 expression can act as an accurate biomarker for GC. This gene can also be an indicator of GC pathogenicity. These results could indicate possible diagnostic and therapeutic applications of BTG1 for GC. | ||
| کلیدواژهها [English] | ||
| BTG1, gastric cancer, biomarkers, diagnosis | ||
| مراجع | ||
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