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بررسی مسیر عملکردی ژنهای هدف miRNAها در پاسخ به تنشهای شوری و خشکی در کلزا | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 12، شماره 1 - شماره پیاپی 39، مهر 1401، صفحه 15-36 اصل مقاله (1.28 M) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2022.66104.1893 | ||
نویسندگان | ||
محمد محسن زاده گلفزانی* 1؛ علیرضا ترنگ2؛ رامین صیقلانی3 | ||
1استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
2دانشیار، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
3مربی، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
چکیده | ||
تنوع و پیچیدگی تنظیم miRNA نشاندهنده اهمیت آنها در فرآیندهای زیستی است و بسیاری از بخشهای تنظیم miRNA میتوانند یک شبکه تنظیمی پیچیده miRNA-mRNA را تشکیل دهند. بنابراین، تحقیق روی شبکههای تنظیمکننده miRNA-mRNA میتواند اطلاعات مفیدی را برای درک فرآیندهای زیستی پیچیده ارائه دهد، که برای مطالعه بیشتر مکانیسمهای تحمل به تنش در گیاهان به خصوص در گیاه کلزا از اهمیت بالایی برخوردار است. در این پژوهش با استفاده از مرور مقالات انجام شده در زمینه تنش های غیر زیستی انتخاب miRNA های مؤثر در تنش خشکی و شوری انجام گرفت و با استفاده از توالیهای مربوط به miRNAهای بالغ و به کمک نرمافزار آنلاین psRNATarget، شناسایی ژنهای هدف انجام شد. لیست ژنی از 225 ژن هدف شناسایی شده با کمک پایگاه UniProt تهیه شد. شناسایی مسیر عملکردی آنها با کمک پایگاه بیوانفورماتیک DAVID و سایتKEGG طبق پارامترهای پیشفرض انجام گرفت. بررسیها نشان داد که این ژنهای هدف در مسیرهای زیستی متعددی ازجمله ریبوزوم، اسپلایسوزوم، پروتئازوم، متابولیسم پورین، متابولیسم سلنوکامپاند و متابولیسم سولفور شرکت داشتند. همچنین به منظور بررسی ژنهای هم بیان از پایگاه داده STRING استفاده شد که نشاندهنده وجود 37 ژن هم بیان در بین ژنهای هدف شناسایی شده بود. | ||
کلیدواژهها | ||
بیوانفورماتیک؛ تنظیم بیان ژن؛ DAVID؛ KEGG؛ miRNA | ||
موضوعات | ||
بیوانفورماتیک | ||
عنوان مقاله [English] | ||
Study of a functional pathway of miRNAs target genes in response to drought ant salt stresses in canola | ||
نویسندگان [English] | ||
Mohammad Mohsenzadeh Golfazani1؛ Alireza Tarang2؛ Ramin Seighalani3 | ||
1Assistant Prof., Department of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. | ||
2Associate Prof. Agricultural Biotechnology Research Institute of Iran (ABRII), North Region Branch, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran | ||
3Instructor. Agricultural Biotechnology Research Institute of Iran (ABRII), North Region Branch, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran | ||
چکیده [English] | ||
There is much information about the regulation of gene expression in response to various stresses at the transcriptional level. Nevertheless, there is limited information about this process at the post-transcriptional level. The diversity and complexity of miRNA regulation indicates their importance in biological processes. Many miRNA regulatory modules can form a complex miRNA-mRNA regulatory network. Therefore, research on miRNA-mRNA regulatory networks can provide valuable information for understanding complex biological processes. These data are very important to further study the stress tolerance mechanisms in plants, especially in rapeseed. In this research, the selection of miRNAs related to drought and salinity stress was made by reviewing the articles on abiotic stresses. Then the target genes were identified using the sequences of mature miRNAs and psRNATarget online software. A gene list of 225 identified target genes was prepared using the UniProt database. Their functional pathway was identified utilizing the DAVID bioinformatics database and KEGG database according to default parameters. Investigations showed that these target genes were involved in several biological pathways including ribosome, spliceosome, proteasome, purine metabolism, selenocompound metabolism, and sulfur metabolism. In addition, the STRING database was used to check co-expression genes. Our result indicated the existence of 37 co-expression genes among the identified target genes. | ||
کلیدواژهها [English] | ||
Bioinformatics, DAVID, KEGG, miRNA, Regulation of gene expression | ||
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