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اثرات محافظتی عصاره هیدروالکلی گیاه خرفه بر فاکتورهای اکسیدانی و آنتیاکسیدانی در آسیب کبدی ناشی از بیسفنول A در موشهای صحرایی نر | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 11، شماره 2 - شماره پیاپی 42، آذر 1401، صفحه 23-31 اصل مقاله (231.65 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2023.62375.1864 | ||
| نویسندگان | ||
| داود فضلی1؛ علی اکبر ملکی راد* 2 | ||
| 1استادیار، گروه زیستشناسی، دانشکده علوم- دانشگاه پیام نور، تهران، ایران | ||
| 2دانشیار، گروه زیستشناسی، دانشکده علوم- دانشگاه پیام نور، تهران، ایران | ||
| چکیده | ||
| گونههای فعال اکسیژن عوامل سیتوتوکسیک هستند که موجب آسیب اکسیداتیو میشوند. بیسفنول A یک آلاینده سمی، اکسیداتیو و شبهاستروژنی است. گیاه خرفه یک محصول امیدوارکننده طبیعی است که میتواند برای جلوگیری از بیماری های مزمن با منشأ استرس اکسیداتیو مفید واقع شود. در این مطالعه 49 سر رت نر بالغ (10±250گرم) بهطور تصادفی به هفت گروه هفتتایی تقسیم شدند؛ گروه کنترل، شاهد (500 میکروگرم روغن ذرت/ کیلوگرم وزن بدن)، گروه دریافتکننده سم (500 میکروگرم بیسفنول A/ کیلوگرم وزن بدن)، کنترل مثبت (100 میلیگرم عصاره هیدروالکلی گیاه خرفه/ کیلوگرم وزن بدن)، سه گروه تیمار دریافتکننده خرفه (500 میکروگرم بیسفنول A+ 50، 100، 150 میلیگرم عصاره هیدروالکلی گیاه خرفه/ کیلوگرم وزن بدن). تمام تزریقات از طریق روش درون صفاقی و طی 14 روز پیاپی انجام شد. سپس تمامی حیوانات با کلرال هیدرات بیهوش شده و نمونههای کبدی جهت تهیه هموژن بافتی جداسازی شدند تا سنجش آنزیمها آنتیاکسیدانی شامل TAC، CAT، GPx، GSH و MDA در این بافت انجام شود. آنالیز دادهها در نرمافزار آماری SPSS توسط روش ANOVA با تست تعقیبی توکی انجام شد. تجزیه و تحلیل دادهها حاکی از آن است که تجزیه و تحلیل دادهها حاکی از آن است که مقادیر MDA، TAC، CAT، GSH و GPX در گروههای تیمار با خرفه نسبت به گروه بیسفنول تعدیل شدهاند که این امر حاکی از آن است که عصاره خرفه میتواند سمیت بافت کبدی ناشی از بیسفنول A را بهبود بخشد. | ||
| کلیدواژهها | ||
| آنتیاکسیدان؛ استرس اکسیداتیو؛ بیسفنول A؛ عصاره خرفه | ||
| عنوان مقاله [English] | ||
| Protective Effects of Hydroalcoholic Extract of Portulaca oleracea on Oxidant and Antioxidant Factors in Male Rats with Bisphenol A -Induced Liver Toxicity | ||
| نویسندگان [English] | ||
| Davoud Fazli1؛ Aliakbar Malekirad2 | ||
| 1Assistant Professor, Department of Biology, Faculty of Science, Payame Nour University (PNU), Tehran, Iran | ||
| 2Associate Professor, Department of Biology, Faculty of Science, Payame Nour University (PNU), P.O.Box, 19395-3697, Tehran, Iran | ||
| چکیده [English] | ||
| Reactive oxygen species (ROS) are cytotoxic agents that lead to significant oxidative damage. Bisphenol A (BPA) is a contaminant toxic, oxidative and estrogenic effects. Purslane is a promising natural product, which could be useful for the prevention of chronic diseases caused by oxidative stress. In this study 49 adult male rats (250 10g) were divided randomly in 7 groups (n=7): Control, Sham (500 ug corn oil\Kg BW), Toxin group (500ug BPA\kg BW), Positive control (100mg purslane hydroalcoholic extract\kg BW) and 3 purslane treated groups (500ug BPA + 50,100&150 mg purslane\kg BW). All injections are done by i.p rout in 14 consequently days. At the end of administration time (14 day) all animals were anesthetized with Chloral hydrate and sacrificed, then liver samples isolated to preparing tissue homogenates for measure antioxidant enzymes including SOD, CAT, TAC, GPX and MDA. Data are analyzed in SPSS statistical software by one way ANOVA with Tukey posttest. Analyzed data showed that MDA, TAC, CAT, GSH and GPX measures are modified in purslane treated groups compared to the bisphenol A group which indicates that the portulaca oleracea extract can improve the hepatotoxicity of bisphenol A induced. | ||
| کلیدواژهها [English] | ||
| Antioxidant, Bisphenol A, Oxidative stress, Purslane extract | ||
| مراجع | ||
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