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اثر عصاره مستوره خوابیده (Eclipta prostrata L.) بر پروفایل لیپیدی، انسولین و ناقل گلوکز ۲ در موشهای دیابتی القاشده با استرپتوزوتوسین | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 14، شماره 1 - شماره پیاپی 53، شهریور 1404، صفحه 1-10 اصل مقاله (858.04 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2025.75656.2006 | ||
| نویسندگان | ||
| شاکر خزیو هویدی الحمداوی1؛ مریم رفیعی راد* 2؛ زهرا شیبانی3 | ||
| 1گروه زیستشناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| 2گروه زیستشناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران | ||
| 3گروه زیستشناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران | ||
| چکیده | ||
| زمینه: دیابت ملیتوس یک اختلال متابولیکی است که با افزایش قند خون و اختلال در پروفایل لیپیدی همراه است و انتقال گلوکز در کبد بیشتر توسط ناقل GLUT2 انجام میشود. هدف: بررسی اثر عصاره گیاه مستوره خوابیده (E. prostrata) بر پروفایل لیپیدی، سطح انسولین و بیان ژن GLUT2 در موشهای دیابتی بود. روش کار: پنجاه موش نر ویستار به پنج گروه تقسیم شدند؛ کنترل سالم، دیابتی و سه گروه تجربی که دوزهای ۵۰، ۱۰۰ و ۲۰۰ میلیگرم بر کیلوگرم عصاره دریافت کردند. پس از ۲۸ روز، نمونههای خونی برای اندازهگیری قند و شاخصهای لیپیدی جمعآوری شد و بیان ژن GLUT2 با PCR بررسی گردید. تحلیل آماری با آزمون ANOVA انجام شد. یافتهها: مصرف عصاره E. prostrata بهطور معناداری کلسترول، تریگلیسرید، LDL و VLDL را کاهش و HDL را افزایش داد. همچنین بیان ژن GLUT2 در بافت کبدی تعدیل شد. نتیجهگیری: عصاره مستوره خوابیده میتواند در کنترل دیابت مؤثر باشد و احتمالاً از طریق فعالسازی مسیرهای سیگنالدهی سلولی و افزایش بیان ژنهای مرتبط با انتقال گلوکز عمل میکند. مطالعات بیشتر برای بررسی مکانیسم دقیق و کاربردهای بالینی فلاونوئیدهای گیاه ضروری است.. | ||
| کلیدواژهها | ||
| مستوره خوابیده؛ پروفایل لیپیدی؛ دیابت؛ GLUT2؛ موش صحرایی | ||
| عنوان مقاله [English] | ||
| The effect of Eclipta prostrata L. extract on lipid profile, insulin, and glucose transporter 2 in streptozotocin-induced diabetic rats | ||
| نویسندگان [English] | ||
| Shaker Khazia Hoveidi Al-Hamdawi1؛ maryam rafieirad2؛ Zahra Shaibani3 | ||
| 1Department of Biology, Shi.C., Islamic Azad University, Shiraz, Iran | ||
| 2Department of Biology, Iz.C., Islamic Azad University, Izeh, Iran | ||
| 3Department of Biology, Payame Noor University, Tehran, Iran | ||
| چکیده [English] | ||
| Background: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and dyslipidemia, with hepatic glucose uptake primarily mediated by the GLUT2 transporter. Objective: To evaluate the effects of E. prostrata extract on lipid profile, insulin levels, and GLUT2 gene expression in diabetic rats. Methods: Fifty male Wistar rats were randomly assigned to five groups: healthy control, diabetic control, and three experimental groups receiving 50, 100, and 200 mg/kg of the extract for 28 days. Blood samples were collected for glucose and lipid measurements, and hepatic GLUT2 expression was analyzed by PCR. Data were analyzed using ANOVA. Results: E. prostrata extract significantly decreased total cholesterol, triglycerides, LDL, and VLDL, while increasing HDL. GLUT2 gene expression in liver tissue was also modulated. Conclusion: E. prostrata may be effective in diabetes management, likely through activation of cellular signaling pathways and upregulation of glucose transporter genes. Further studies are needed to clarify its precise mechanisms and the clinical potential of its flavonoids. | ||
| کلیدواژهها [English] | ||
| Eclipta prostrata, Lipid profile, Diabetes, GLUT2, Rat | ||
| مراجع | ||
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Adeyi, A. O., Idowu, B. A., Mafiana, C. F., Oluwalana, S. A., Ajayi, O. L., & Akinloye, O. A. (2012). Rat model of food-induced non-obese-type 2 diabetes mellitus: comparative pathophysiology and histopathology. Int J Physiol Pathophysiol Pharmacol, 4(1), 51-58.
Akcilar, R., Kocak, F. E., Simsek, H., Akcilar, A., Bayat, Z., Ece, E., & Kokdasgil, H. (2016). Antidiabetic and hypolipidemic effects of adropinin streoptozotocin-induced type 2 diabetic rats. Bratisl Lek Listy, 117(2), 100-105. https://doi.org/4941.10/bll_2016_020
Al-Ishaq, R. K., Abotaleb, M., Kubatka, P., Kajo, K., & Büsselberg, D. (2019). Flavonoids and Their Anti-Diabetic Effects: Cellular Mechanisms and Effects to Improve Blood Sugar Levels. Biomolecules, 9(9). https://doi.org/10.3390/biom9090430
Amiri, M. S., Yazdi, M. E. T., & Rahnama, M. (2021). Medicinal plants and phytotherapy in Iran: Glorious history, current status and future prospects. Plant Science Today, 8(1), 95-111. https://doi.org/10.14719/pst.2021.8.1.926
Antoine, B., Lefrançois-Martinez, A. M., Le Guillou, G., Leturque, A., Vandewalle, A., & Kahn, A. (1997). Role of the GLUT 2 glucose transporter in the response of the L-type pyruvate kinase gene to glucose in liver-derived cells. J Biol Chem, 272(29), 17937-17943. https://doi.org/10.1074/jbc.272.29.17937
Asmat, U., Abad, K., & Ismail, K. (2016). Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal, 24(5), 547-553. https://doi.org/https://doi.org/10.1016/j.jsps.2015.03.013
Cazzo, E., Jimenez, L. S., Gestic, M. A., Utrini, M. P., Chaim, F. H. M., Chaim, F. D. M., Pareja, J. C., & Chaim, E. A. (2018). Type 2 Diabetes Mellitus and Simple Glucose Metabolism Parameters may Reliably Predict Nonalcoholic Fatty Liver Disease Features. Obes Surg. 28(1), 187-194. https://doi.org/10.1007/s11695-017-2829-9
Chahardoli, M., Mahmoodi, M., Hajizadeh, M. R., Khoramdel Azad, H., Khoshdel, A. R., & Mirzaei, M. R. (2015). Effect of Aloe Vera Hydroalcoholic Extract on Blood Glucose, Serum Insulin and the Key Enzymes in Metabolic Pathways of Glycolysis and Gluconeogenesis in Hepatocytes of Type 1 Diabetic Rats [Research]. Journal of Rafsanjan University of Medical Sciences, 13(8), 669-682.
Chunudom, L., Thongsom, M., Karim, N., Rahman, M. A., Rana, M. N., & Tangpong, J. (2020). Tithonia diversifolia aqueous fraction plays a protective role against alloxan-induced diabetic mice via modulating GLUT2 expression. South African Journal of Botany, 133, 118-123. https://doi.org/https://doi.org/10.1016/j.sajb.202007.07
Dihingia, A., Ozah, D., Ghosh, S., Sarkar, A., Baruah, P. K., Kalita, J., Sil, P. C., & Manna, P. (2018). Vitamin K1 inversely correlates with glycemia and insulin resistance in patients with type 2 diabetes (T2D) and positively regulates SIRT1/AMPK pathway of glucose metabolism in liver of T2D mice and hepatocytes cultured in high glucose. J Nutr Biochem, 52, 103-114. https://doi.org/10.1016/j.jnutbio.2017.09.022
Dimitriadis, G. D., Maratou, E., Kountouri, A., Board, M., & Lambadiari, V. (2021). Regulation of Postabsorptive and Postprandial Glucose Metabolism by Insulin-Dependent and Insulin-Independent Mechanisms: An Integrative Approach. Nutrients, 13(1). https://doi.org/10.3390/nu13010159
Feng, L., Zhai, Y. Y., Xu, J., Yao, W. F., Cao, Y. D., Cheng, F. F., Bao, B. H., & Zhang, L. (2019). A review on traditional uses, phytochemistry and pharmacology of Eclipta prostrata (L.). J Ethnopharmacol, 245, 112109. https://doi.org/10.1016/j.jep.2019.112109
Freitas, H. S., Schaan, B. D., Seraphim, P. M., Nunes, M. T., & Machado, U. F. (2005). Acute and short-term insulin-induced molecular adaptations of GLUT2 gene expression in the renal cortex of diabetic rats. Mol Cell Endocrinol, 237(1-2), 49-57. https://doi.org/10.1016/j.mce.2005.03.005
Gaeini, A., Ramezani, N., & Shafiei Neek, L. (2019). Changes of LXR α, GLUT2 genes expression in liver and insulin resistance after aerobic training in type 2 diabetic rats. Metabolism and Exercise, 9(1), 1-13. https://doi.org/10.22124/jme.2020.4352
Hosokawa, M., Dolci, W., & Thorens, B. (2001). Differential Sensitivity of GLUT1- and GLUT2-Expressing β Cells to Streptozotocin. Biochemical and Biophysical Research Communications, 289(5), 1114-1117. https://doi.org/https://doi.org/10.1006/bbrc.2001.6145
Kumar, S., Kumari, B., Kaushik, A., Banerjee, A., Mahto, M., & Bansal, A. (2022). Relation Between HbA1c and Lipid Profile Among Prediabetics, Diabetics, and Non-diabetics: A Hospital-Based Cross-Sectional Analysis. Cureus, 14(12), e32909. https://doi.org/10.775/9cureus.32909
Low, B. S. J., Lim, C. S., Ding, S. S. L., Tan, Y. S., Ng, N. H. J., Krishnan, V. G., Ang, S. F., Neo, C. W. Y., Verma, C. S., Hoon, S., Lim, S. C., Tai, E. S., & Teo, A. K. K. (2021). Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Nature Communications, 12(1), 3133. https://doi.org/10.1038/s41467-021-22843-4
Maritim, A. C., Sanders, R. A., & Watkins, J. B. (2003). Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol, 17(1), 24-38. https://doi.org/10.1002/jbt.10058
Marks, J., Carvou, N. J., Debnam, E. S., Srai, S. K., & Unwin, R. J. (2003). Diabetes increases facilitative glucose uptake and GLUT2 expression at the rat proximal tubule brush border membrane. J Physiol, 553(Pt 1), 137-145. https://doi.org/10.1113/jphysiol.2003.046268
Maryam, R., Abdolhassan, D., & Samira, G. (2022). Evaluation of the Effect of Alpha-pinene on Blood Glucose and Lipid Profiles, in Diabetic Rats. Journal of Animal Biology, 14(3), 171-180.
Okamoto, Y., Tanaka, S., & Haga, Y. (2002). Enhanced GLUT2 gene expression in an oleic acid-induced in vitro fatty liver model. Hepatol Res, 23(2), 138-144. https://doi.org/10.1016/s1386-6346(01)00172-3
Poyil, M. M., Alsharif, M. H. K., El-Bidawy, M. H., Bin Dayel, S., Khan, M. S., Omar, Z. M. M., Mohamed, A. A., Fayyad, R. M., Alarabi, T. G. M., Khairy, H. A., Bahakim, N. O., Samhan, M. A., & El-Lateef, A. (2024). Anti-Inflammatory Potential and Synergic Activities of Eclipta prostrata (L.) Leaf-Derived Ointment Formulation in Combination with the Non-Steroidal Anti-Inflammatory Drug Diclofenac in Suppressing Atopic Dermatitis (AD). Life (Basel), 15(1). https://doi.org/10.3390/life15010035
Rathinam, A., & Pari, L. (2016). Myrtenal ameliorates hyperglycemia by enhancing GLUT2 through Akt in the skeletal muscle and liver of diabetic rats. Chem Biol Interact, 256, 161-166. https://doi.org/10.1016/j.cbi.2016.07.009
Roy, J. R., Janaki, C. S., Jayaraman, S., Veeraraghavan, V. P., Periyasamy, V., Balaji, T., Vijayamalathi, M., Bhuvaneswari, P., & Swetha, P. (2023). Hypoglycemic Potential of Carica papaya in Liver Is Mediated through IRS-2/PI3K/SREBP-1c/GLUT2 Signaling in High-Fat-Diet-Induced Type-2 Diabetic Male Rats. Toxics, 11(3). https://doi.org/10.3390/toxics11030240
Shah, N. P., Wang, Q., Wolski, K. E., Cho, L., McErlean, E., Ruotolo, G., Weerakkody, G., Riesmeyer, J. S., Nicholls, S. J., Lincoff, A. M., Nissen, S. E., & Menon, V. (2020). The Role of Lipoprotein (a) as a Marker of Residual Risk in Patients With Diabetes and Established Cardiovascular Disease on Optimal Medical Therapy: Post Hoc Analysis of ACCELERATE. Diabetes Care, 43(2), e22-e24. https://doi.org/10.2337/dc19-1117
Shahrbano, A., & Maryam, R. (2023). Investigating the effect of Eclipta prostrata hydroalcoholic extract on motor activity, avoidance memory and oxidative stress in an animal model of Parkinson's disease in adult male rats. Journal of Plasma and Biomarkers, 16(3), 1-18.
Shaibani, Z., & Rafieirad, M. (2024). Protective Effect of Oleuropein on Memory Impairment and Oxidative Stress in Streptozotocin-Induced Diabetes Rats via Modulation of NF-kB and Nrf-2 Pathways [Research]. Journal of Advanced Biomedical Sciences, 14(2), 115-127. https://doi.org/10.18502/jabs.v14i2.15756
Sok Yen, F., Shu Qin, C., Tan Shi Xuan, S., Jia Ying, P., Yi Le, H., Darmarajan, T., Gunasekaran, B., & Salvamani, S. (2021). Hypoglycemic Effects of Plant Flavonoids: A Review. Evid Based Complement Alternat Med, 2021, 2057333. https://doi.org/10.1155/2021/2057333
Sun, B., Chen, H., Xue, J., Li, P., & Fu, X. (2023). The role of GLUT2 in glucose metabolism in multiple organs and tissues. Mol Biol Rep, 50(8), 6963–6974. https://doi.org/10.1007/s11033-023-08535-w
Valizadeh, Z., & Rafieirad, M. (2016). Effects of Hydro-Alcoholic Leaf Extract of Kardeh (Biarum Bovei Blume) on the Blood Glucose and Lipid Peroxidation in Cerebral Tissues and Lipid Profile in Streptozotocin Induced Diabetic Rats [Research]. Iranian Journal of Diabetes and Obesity, 8(1), 16-23.
Velasco-Amador, J. P., Prados-Carmona, Á., & Navarro-Triviño, F. J. (2023). Medical Devices in Patients With Diabetes and Contact Dermatitis. Actas Dermosifiliogr. https://doi.org/10.1016/j.ad.2023.10.011 (Original work published Dispositivos médicos en pacientes diabéticos y dermatitis de contacto).
Yamamoto, T., Fukumoto, H., Koh, G., Yano, H., Yasuda, K., Masuda, K., Ikeda, H., Imura, H., & Seino, Y. (1991). Liver and muscle-fat type glucose transporter gene expression in obese and diabetic rats. Biochem Biophys Res Commun, 175(3), 995–1002. https://doi.org/10.1016/0006-291x(91)91663-w
Yang, D. K., & Kang, H. S. (2018). Anti-Diabetic Effect of Cotreatment with Quercetin and Resveratrol in Streptozotocin-Induced Diabetic Rats. Biomol Ther (Seoul), 26(2), 130–138. https://doi.org/10.4062/biomolther.2017.254
Yonamine, C. Y., Pinheiro-Machado, E., Michalani, M. L., Alves-Wagner, A. B., Esteves, J. V., Freitas, H. S., & Machado, U. F. (2017). Resveratrol Improves Glycemic Control in Type 2 Diabetic Obese Mice by Regulating Glucose Transporter Expression in Skeletal Muscle and Liver. Molecules, 22(7). https://doi.org/10.3390/molecules22071180
Yonamine, C. Y., Pinheiro-Machado, E., Michalani, M. L., Freitas, H. S., Okamoto, M. M., Corrêa-Giannella, M. L., & Machado, U. F. (2016). Resveratrol improves glycemic control in insulin-treated diabetic rats: participation of the hepatic territory. Nutrition & Metabolism, 13(1), 44. https://doi.org/10.1186/s12986-016-0103-0
Zar, A., Hoseini, A., Ahmadi, F., & Rezaei, M. (2016). Effects of Ginger together with Swimming Training on Blood Fat Profiles in Adult Diabetic Rats with Streptozotocin [Research]. Iranian Journal of Nutrition Sciences and Food Technology, 11(2), 65-74. | ||
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