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بررسی بیوانفورماتیکی پروتئین (SQS) در پروکاریوت‌ها و یوکاریوت‌ها | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 مهر 1402 | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2023.69132.1925 | ||
| نویسندگان | ||
| مریم مهدیزاده حکاک1؛ مسعود توحیدفر* 2؛ محمدحسین میرجلیلی3 | ||
| 1دانشجوی دکتری بیوتکنولوژی کشاورزی گروه زیست شناسی سلولی و مولکولی، دانشکده علوم و فنآوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| 2گروه بیوتکنولوژِی و علوم زیستی، دانشکده علوم و فنآوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| 3گروه مهندسی کشاورزی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، تهران، ایران. | ||
| چکیده | ||
| بررسی بیوانفورماتیکی اسکوالن یوکاریوت تک‌سلولی و پرسلولی و پروکاریوت | ||
| کلیدواژه‌ها | ||
| آنالیز فیلوژنتیکی؛ پروتئین SQS؛ درصد GC؛ شاخص آلیفاتیک؛ مدل سازی سه بعدی | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| Bioinformatic Investigation Of Protein Stability (SQS) In Prokaryotes and Eukaryotes | ||
| نویسندگان [English] | ||
| maryam mehdizadeh hakkak1؛ masoud tohidfar2؛ Mohammad Hossein Mirjalili3 | ||
| 1Phd Student of Agricultural Biotechnology, Biotechnology and Life Sciense Department, Shahid Beheshti University of Tehran, Iran | ||
| 2Biotechnology and Life Sciense Department, Shahid Beheshti University of Tehran, Iran | ||
| 3Department of Agricultural Engineering, Research Institute of Medicinal Plants and Raw Materials, Shahid Beheshti University, Tehran, Iran. | ||
| چکیده [English] | ||
| In this research, the production of squalene and its bioinformatic analysis in unicellular and multicellular eukaryotes and prokaryotes were investigated in order to determine the difference of this gene in eukaryotes and prokaryotes. The results of phylogenetic analysis showed that algae as multicellular eukaryotes, yeast as single-celled eukaryote and bacteria as single-celled prokaryote were placed in one group and plants were placed in one group. GC percentage of SQS protein was evaluated by GC Content Calculator, as well as aliphatic index and instability index by protparam. Results showed that the SQS gene is in a range from unstable to stable. The analysis of the presence of signal sequences and the analysis of the detection of the final location of the protein showed that the possibility of transferring the SQS protein to the mitochondria, chloroplast and secretory pathway is very low and it is not among the signal proteins. It was also found in Gymnema sylvestre that this protein has three protected domains. The comparison of the secondary structure of the protein confirmed the existence of alpha sheets. 3D modeling of this protein in plant was done by homology modeling method and using Swiss Model database after selecting a suitable model with high similarity which was extracted from PDB database. In order to validate the structure of the drawn three-dimensional model and stereochemical analysis, the Ramachandran diagram was drawn and the dihedral angles were calculated. The results of structural quality evaluation showed that the proposed models have good quality and stability. | ||
| کلیدواژه‌ها [English] | ||
| Aliphatic Index, 3D modeling, GC Percentage, Phylogenetic Analysis, SQS protein | ||
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آمار تعداد مشاهده مقاله: 92 |
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