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شناسایی ژنهای خانواده HSP60 در گستره ژنوم سویا و بررسی کارکردی آنها در پاسخ به تنشهای غیرزیستی | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
دوره 13، شماره 1 - شماره پیاپی 43، مهر 1402، صفحه 11-28 اصل مقاله (2.51 M) | ||
نوع مقاله: علمی پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/cb.2023.69628.1931 | ||
نویسنده | ||
سمیرا محمدی* | ||
گروه به نژادی گیاهی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری | ||
چکیده | ||
پروتئینهای شوک حرارتی 60 کیلو دالتونی (HSP60) که بهعنوان چاپرونین (cpn60) نیز شناخته میشوند، نقش مهمی در رشد و نمو و پاسخ گیاه به تنش ایفا مینمایند. در این مطالعه از طریق ابزارهای بیوانفورماتیکی، 32 ژن HSP60 در ژنوم سویا شناسایی شد که روی 14 کروموزوم توزیع شدهاند. بیشتر این پروتئینها آبدوست، اسیدی، ناپایدار با شاخص آلیفاتیک بالا هستند. درخت تکاملی، پروتئینهای HSP60 سویا، آرابیدوپسیس و برنج را بر مبنای جایگاه سلولی در سه گروه اصلی تقسیمبندی نمود. پروتئینهای واقع در زیرگروههای مختلف از نظر ساختار ژنی، موتیفهای حفاظتشده، فاز اینترون و ساختار سهبعدی از حفاظتشدگی بالایی برخوردار بوده که این امر میتواند بیانگر شباهتهای کارکردی آنها در زیرگروههای مختلف باشد. چندین عنصر تنظیمی سیس پاسخگو به تنشها، رشد و نمو و هورمونها در پروموتر ژنهای GmHSP60 یافت شد که بیانگر نقش آنها در رشد و نمو و پاسخ گیاه به تنشهای محیطی میباشد. تجزیه و تحلیل هستی شناسی ژن (GO)، پیشبینی کرد که ژنهای GmHSP60 در پاسخ به تنشهای مختلف، مسئول تاخوردگی و تاخوردگی مجدد پروتئین به روشی وابسته به ATP هستند. بررسی الگوی ترانسکریپتوم (RNA-Seq) نشان داد که بیشتر ژنهای GmHSP60 دارای بیان بالایی در پاسخ به تنشهای شوری، خشکی، سرما، گرما، غرقاب و کمبود مواد غذایی بودند که بیانگر نقش آنها در افزایش تحمل سویا به تنشهای غیرزیستی میباشد. بهطور کلی، این یافتهها اطلاعات مفیدی را برای درک بهتر کارکرد ژنهای GmHSP60 در سویا فراهم آورده و راه را برای استفاده از ژنهای خانواده چاپرونین برای دستیابی به تحمل گیاهان در برابر تنشهای غیرزیستی تسهیل مینمایند. | ||
کلیدواژهها | ||
پروتئین شوک حرارتی؛ چاپرونین؛ ساختار سهبعدی؛ عناصر تنظیمی سیس؛ هستیشناسی ژن | ||
موضوعات | ||
بیوانفورماتیک | ||
عنوان مقاله [English] | ||
Identification of HSP60 family gene in the soybean genome and their functional analysis in response to abiotic stresses | ||
نویسندگان [English] | ||
Samira Mohammadi | ||
Department of Plant Biotechnology and breeding, Sari Agricultural Sciences and Natural Resources University | ||
چکیده [English] | ||
60 kDa heat shock proteins (HSP60s) also known as chaperonin (cpn60) play an important role in plant growth and stress response. In this study, 32 HSP60 genes were identified in the soybean genome through bioinformatics tools, which are distributed on 14 chromosomes. Most of these proteins are hydrophilic, acidic, and unstable with a high aliphatic index The evolutionary tree divided HSP60 proteins of soybean, Arabidopsis, and rice into three main groups based on their cellular location. The proteins of different subgroups have highly conserved gene structure, conserved motifs, intron phase, and three-dimensional structure, which can indicate their functional similarities in different subgroups. Several cis-regulatory elements responsive to stresses, growth and hormones were found in the promoter of GmHSP60 genes, that indicate their role in plant growth and response to environmental stresses. Gene ontology (GO) analysis predicted that GmHSP60 genes were responsible for protein folding and refolding in an ATP-dependent manner in response to various stresses. Analysis of the transcriptome pattern (RNA-seq) showed that most of the GmHSP60 genes had high expression in response to salt, drought, cold, heat, submergence, and nutrient deficiency stresses, which indicates their role in improving soybean tolerance to abiotic stresses. Overall, these findings provide useful information to better understand the function of GmHSP60 genes in soybean and facilitate the way for the utilization of chaperonin family genes for achieving plant tolerance against abiotic stresses. | ||
کلیدواژهها [English] | ||
Chaperonin, Cis-regulatory elements, Gene ontology, Heat shock protein, Three-dimensional structure | ||
مراجع | ||
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