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بررسی عملکرد فرمولاسیونهای لیپوزومی حاوی اسانس میخک ساخته‌شده بهروش لایه نازک و گرمایی در شرایط دمایی و pH مشابه دهان به منظور کاربرد در تحقیقات بیماریهای دهان و دندان | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 12، شماره 2 - شماره پیاپی 46، آذر 1402، صفحه 15-26 اصل مقاله (1.05 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2024.68281.1914 | ||
| نویسندگان | ||
| مریم زارع زاده مهریزی1؛ محمد مجدی زاده* 2؛ محبوبه زارع زاده مهریزی1؛ علیرضا افشون3؛ مهتاب ژیان مهریزی3؛ بی بی فاطمه حقیرالسادات3، 4 | ||
| 1دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید صدوقی یزد، یزد، ایران | ||
| 2گروه زیست‌فناوری، شرکت دانش بنیان ریز زیست‌فناوران فردانگر، پارک علم و فناوری، یزد، ایران | ||
| 3گروه علوم و فنون نوین پزشکی، دانشکده پیراپزشکی، دانشگاه علوم پزشکی و خدمات درمانی شهید صدوقی یزد، ایران | ||
| 4مرکز تحقیقات نانوتکنولوژی پزشکی و مهندسی بافت، پژوهشکده علوم تولیدمثل، دانشگاه علوم پزشکی شهید صدوقی یزد، ایران | ||
| چکیده | ||
| با توجه به مشکلات استفاده از گیاهان دارویی در درمان بیماریها از جمله فراریت و اثرگذاری غیر هدفمند و نقش فناوری نانو در کاهش این چالشها، پژوهش حاضر با هدف دستیابی به فرمولاسیون نانویی حاوی اسانس میخک به منظور استفاده در تحقیقات دندانپزشکی انجام شد. چهار فرمولاسیون لیپوزومی حاوی اسانس میخک با استفاده از فسفاتیدیل کولین و کلسترول بهروش لایه نازک و گرمایی بهصورت جداگانه تهیه شد. بازده بارگذاری، اندازه، شاخص پراکندگی و پتانسیل زتا نانوذرت (با استفاده از زتا سایزر) سنجیده شد. در هر کدام از روشهای ساخت، فرمولاسیونی که شاخصههای فیزیکوشیمیایی مناسبی داشت به منظور بررسی الگوی رهایش اسانس و شکل ظاهری نانوذرات(با استفاده از میکروسکوپ الکترونی روبشی)، انتخاب گردید. همچنین سمیت نانولیپوزومهای حاصل از فرمولاسیونهای منتخب، به روش MTT بر رده سلولی فیبروبلاست انسانی (HFF) سنجیده شد. بازده بارگذاری، اندازه ذرات و پتانسیل زتا برای فرمولاسیون منتخب حاصل از روش لایه نازک به ترتیب 57/50%، nm3/123 و mV6/18- بود و برای روش گرمایی به ترتیب 43/55%، nm4/121 و mV2/19- بود. رهایش اسانس از فرمولاسیونهای منتخب آهسته و پیوسته و وابسته به pH بود. نانولیپوزومهای ساخته شده بهروش گرمایی نسبت بهروش لایه نازک سمیت کمتری برای رده سلولی HFF ایجاد نمودند. با توجه ویژگی‌های فیزیکوشیمیایی مناسب نانولیپوزومهای حاوی اسانس میخک و سمیت بسیار کم نانولیپوزومهای ساخته شده بهروش گرمایی برای سلولهای سالم HFF، میتوان این نانو لیپوزومها را برای انجام تحقیقات بیشتر در حوزه بهداشت دهان و دندان پیشنهاد نمود. | ||
| کلیدواژه‌ها | ||
| نانو لیپوزوم؛ میخک؛ دندانپزشکی؛ روش لایه نازک؛ روش گرمایی | ||
| عنوان مقاله [English] | ||
| Investigating the performance of liposomal formulations containing clove essential oil made by thin layer and heat method in temperature and pH conditions similar to the mouth in order to be used in the research of oral and dental diseases | ||
| نویسندگان [English] | ||
| Maryam Zarehzadeh-Mehrizi1؛ Mohammad Majdizadeh2؛ Mahboubeh Zarezadeh-Mehrizi1؛ Alireza Afshoun3؛ Mahtab Zhian- Mehrizi3؛ Bibi Fatemeh Haghiralsadat3، 4 | ||
| 1Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran | ||
| 2Nano-Biotech Foresight Company Biotechnology Campus, Science & Technology Park of Yazd, Yazd, Iran | ||
| 3Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran | ||
| 4Medical nanotechnology & tissue engineering research center, Yazd reproductive sciences institute, shahid sadoughi university of medical sciences, Yazd, Iran|Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran | ||
| چکیده [English] | ||
| Considering the problems of using medicinal plants in the treatment of diseases such as high volatility and non-targeted effects, and the role of nanotechnology in reducing these challenges, the present research was conducted with the aim of obtaining a nano formulation containing Clove essential oil for use in dental research. 4 liposomal formulations containing Clove essential oil were prepared using phosphatidylcholine and cholesterol by thin layer and heat methods separately. The encapsulation efficiency, size, polydispersity-index and zeta potential of nanoparticle (by zeta-sizer) were measured. In each of the manufacturing methods, a formulation that had suitable physicochemical parameters was selected in order to check the essential oil release pattern and the morphology of nanoparticles (by SEM). Also, the toxicity of nano-liposomes obtained from selected formulations was measured by MTT method on human fibroblast(HFF) cell line. The encapsulation efficiency, particle size and zeta potential for the selected formulation obtained from the thin layer method were 50.57%, 123.3nm and -18.6mV, respectively, and for the heat method, they were 55.43%, 121.4nm respectively and -19/2mV. The release of essential oil from the selected formulations was slow and continuous and dependent on pH. Nano-liposomes made by heat method caused less toxicity to HFF cell line compared to thin layer method. Considering the suitable physicochemical characteristics of nano-liposomes containing Clove essential oil and very low toxicity of nano-liposomes made by heat method for healthy HFF cells, these nano-liposomes can be suggested to further research in the field of oral and dental health. | ||
| کلیدواژه‌ها [English] | ||
| Nano-Liposome, Syzygium aromaticum(Clove), Dental, Thin layer method, Heat method | ||
| مراجع | ||
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