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بررسی بیان miRNAهای کنترل کننده فاکتورهای رونویسی مرتبط با مسیرهای سیگنالینگ اکسین، جیبرلین و اسید آبسزیک، تحت شرایط تنش خشکی در گندم (Triticum aestivum L.) | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 3، دوره 3، شماره 3 - شماره پیاپی 6، شهریور 1393، صفحه 21-33 اصل مقاله (520.32 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| مهدیه صفرزاده* 1؛ رضا فتوت2؛ محمد رضا عظیمی2؛ احسان محسنی فرد3؛ بهنام بخشی3 | ||
| 1کارشناس‌ارشد، بخش ژنومیکس، پژوهشکده بیوتکنولوژی کشاورزی ایران، کرج، | ||
| 2استادیار، دانشکده کشاورزی، دانشگاه زنجان، زنجان. | ||
| 3دانشجوی دکتری، بخش ژنومیکس، پژوهشکده بیوتکنولوژی کشاورزی ایران، کرج. | ||
| چکیده | ||
| رشد و متابولیسم گیاه تحت تأثیر انواع محرک‌های زنده و غیرزنده از جمله تنش‌های محیطی قرار می‌گیرد که گیاه از طریق هورمون‌ها به آن‌ها پاسخ می‌دهد. miRNAها، گروهی از RNAهای کوچک غیر کدکننده هستند که برخی از آن‌ها در سیگنالینگ هورمون‌های گیاهی نقش دارند. در این مطالعه با استفاده از تکنیک qRT-PCR، الگوی بیان miR159a,b، miR160، miR167a,b و miR171a، که به‌ترتیب در تنظیم بیان فاکتورهای رونویسی MYB، ARF، ARF، SCL نقش دارند، در دو رقم حساس و متحمل به تنش خشکی در گندم مورد بررسی قرار گرفتند. بررسی میزان شباهت نوکلئوتیدی نشان داد که بیشترین شباهت در هر یک از این خانواده‌ها در ناحیه تولید کننده miRNA بالغ می‌باشد. آنالیزهای qRT-PCR نشان داد تحت شرایط تنش خشکی miR159a,bدر رقم حساس و miR160 و miR167a,b در رقم متحمل افزایش بیان معنی‌داری داشتند. تحت این شرایط در miR171a تغییر بیان معنی‌داری در هر دو رقم مشاهده نشد. احتمالاً افزایش بیان miR159a,b در رقم حساس منجر به کاهش پاسخ ژن‌های MYB درگیر در تنش خشکی خواهد شد. افزایش بیان miR160 و miR167a,b در رقم متحمل، باعث تنظیم اثر متقابل مسیرهای سیگنالینگ اکسین و آبسیزیک اسید و احتمالاً کمک به تحمل تنش، در رقم متحمل به تنش خشکی می‌شوند. همچنین با عدم تغییر بیان miR171a در هر دو رقم، احتمالاً تحت این شرایط بیان ژن‌های SCL از طریق سایر مکانیسم‌های گیاهی تنظیم می‌شوند. | ||
| کلیدواژه‌ها | ||
| miRNA؛ سیگنالینگ هورمون؛ گندم؛ تنش خشکی | ||
| موضوعات | ||
| اصلاح نباتات مولکولی؛ بیوانفورماتیک؛ بیوتکنولوژی و تنش های زنده و غیرزنده؛ ژنومیکس | ||
| عنوان مقاله [English] | ||
| Expression Analysis Of miRNAs That Regulate Transcription factors related to Auxin, Gibberellin And ABA Signaling Pathways, Under Water Stress In Wheat (Triticum aestivum L.) | ||
| نویسندگان [English] | ||
| Mahdieh Safarzadeh1؛ Reza Fotovat2؛ Mohammadreza Azimi2؛ Ehsan Mohsenifard3؛ Behnam Bakhshi3 | ||
| 1M.Sc. Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran. | ||
| 2Assistant Professors, Faculty of Agriculture, University of Zanjan, Zanjan, Iran. | ||
| 3Ph.D. Student, Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran. | ||
| چکیده [English] | ||
| Growth and metabolism of plants are affected by a variety of stimuli, including biotic and abiotic environmental stresses that could leads to responses of the plant through hormone regulation. miRNAs, are a group of Non-coding RNAs that some of them are involved in signaling of plant hormones. In this study, the expression patterns of miR159a,b, miR160, miR167a,b and miR171a have been studied in both drought susceptible and drought tolerant varieties in wheat using qRT-PCR. miR159a,b, miR160, miR167a,b and miR171a could play important roles in MYB, ARF, ARF, and SCL, transcription factors regulation, respectively. High conservation among the studied miRNA families was observed in the mature miRNA producer regions by multiplex alignment of pre-miRNAs. Results of qRT-PCR analysis indicated that expressions of miR160 and miR167a,b in tolerant Variety and miR159a,b in susceptible Variety are increased significantly. However, no significant changes in expression were observed for miR171a in both tolerant and sensitive varieties. Presumably, up-regulation miR159a,b in susceptible variety could be resulted to reduction in the expression of MYB genes involved in drought response. On the other hand, up-regulation of miR160 and miR167a,b in tolerant variety, may lead to regulation of auxin and abscisic acid pathways interaction and probably these miRNAs could contribute in stress tolerance in tolerant variety. In addition, no significant change in miR171a expression demonstrated that expression of SCL could be regulated through other mechanisms in plant. | ||
| کلیدواژه‌ها [English] | ||
| miRNA, Hormone signaling, Wheat, Drought stress | ||
| مراجع | ||
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