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ارزیابی تنوع ژنتیکی آفتابگردان آجیلی (Helianthus annuus L.) با استفاده از نشانگرهای ریزماهواره | ||
فصلنامه علمی زیست فناوری گیاهان زراعی | ||
مقاله 6، دوره 3، شماره 2 - شماره پیاپی 6، شهریور 1393، صفحه 61-72 اصل مقاله (367.62 K) | ||
نوع مقاله: علمی پژوهشی | ||
نویسندگان | ||
مرجان جنت دوست1؛ رضا درویش زاده* 2؛ محمدعلی ابراهیمی3 | ||
1دانشآموخته کارشناسی ارشد بیوتکنولوژی کشاورزی، پژوهشکده زیستفناوری دانشگاه ارومیه، | ||
2دانشیار گروه اصلاح و بیوتکنولوژی گیاهی دانشکده کشاورزی دانشگاه ارومیه، | ||
3دانشیار گروه بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران. | ||
چکیده | ||
تنوع ژنتیکی بین و درون 50 توده آفتابگردان آجیلی با استفاده از 10 نشانگر ریزماهواره مورد ارزیابی قرار گرفت. تعداد آلل در مکانهای ژنی بین 2 تا 3 با میانگین 1/2 آلل به ازای هر مکانژنی بود. تعداد آلل مؤثر بین 948/1 در مکانژنی ORS785 و 468/1 در مکانژنی ORS1088 با میانگین 825/1 آلل برآورد شد. بیشترین میزان هتروزیگوسیتی مورد انتظار (484/0) در مکانژنی ORS785 و کمترین آن (268/0) در مکانژنی ORS1088 برآورد شد. میانگین هتروزیگوسیتی مورد انتظار و مشاهده شده به ترتیب برابر 435/0 و 722/0 بود. محتوای اطلاعات چند شکلی (PIC) بین 573/0 در مکانژنی ORS785 و 334/0 در مکانژنی ORS1088 با میانگین 503/0 برآورد گردید. تجزیه به مختصات اصلی (PCoA) نشان داد دو مؤلفه اصلی اول مجموعا 71/31% از واریانس کل را توجیه می نمایند. گروهبندی تودههای آفتابگردان آجیلی مورد مطالعه براساس ضریب فاصله ژنتیکی نی و با استفاده از الگوریتم Complete، آنها را در 3 گروه مجزا قرار داد. تجزیه واریانس مولکولی نشان داد تنوع درون تودهها بیشتر از تنوع بین تودها میباشد. بنابراین در برنامههای بهنژادی آفتابگردان آجیلی، میتوان انتخاب را درون تودهها انجام داد. | ||
کلیدواژهها | ||
آفتابگردان؛ تجزیه واریانس مولکولی؛ تجزیه خوشهای؛ محتوای اطلاعات چند شکل؛ نشانگرهای مولکولی | ||
موضوعات | ||
ژنومیکس | ||
عنوان مقاله [English] | ||
Studying genetic diversity in confectionary sunflower (Helianthus annuus L.) by using microsatellit markers | ||
نویسندگان [English] | ||
Marjan Jannatdoust1؛ Reza Darvishzadeh2؛ Mohammad Ali Ebrahimi3 | ||
1Institute of Biotechnology, Urmia University, Urmia, Iran | ||
2Associate Professor, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran, | ||
3Associate Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. | ||
چکیده [English] | ||
In this study, genetic variability among and within 50 confectionery sunflower populations was investigated using 10 microsatellite markers (SSR). The number of alleles per locus ranged from 2 to 3 with an average of 2.1. The number of effective alleles per locus was estimated from 1.948 (locus ORS785) to 1.468 (locus ORS1088) with an average of 1.825. The highest expected heterozygosity (0.484) was estimated for ORS785 locus and the lowest one (0.268) estimated for ORS1088. The mean of expected and observed heterozygosity was 0.435 and 0.722, respectively. Polymorphic information content varied between 0.572 in ORS785 locus and 0.334 in ORS1088 with an average of 0.503. A principal co-ordinates analysis (PCoA) showed that the first two Eigen-values explained 31.71% of the cumulative variation. Cluster analysis based on complete algorithm and Nei genetic distance grouped the studied populations in 3 main classes. Analysis of molecular variance revealed that the high part of total variation was due to within populations. So it will be better to do selection within populations in breeding programs. | ||
کلیدواژهها [English] | ||
sunflower, analysis of molecular variance, Cluster analysis, Polymorphic information content, Molecular markers | ||
مراجع | ||
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