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بررسی فعالیت‌های زیستی سلول‌های بنیادی مزانشیمی کشت‌شده بر روی بستر پوششی مشتق‌شده از ماهیچه اسکلتی | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 12، شماره 3 - شماره پیاپی 47، اسفند 1402، صفحه 1-13 اصل مقاله (1.5 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2024.70348.1937 | ||
| نویسندگان | ||
| فایزه زارعان1؛ سمیه عرب زاده2؛ سارا رجبی3؛ محمود تلخابی* 1؛ | ||
| 1گروه علوم جانوری و زیست‌شناسی دریا، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| 2گروه زیست‌شناسی، دانشکده علوم پایه، مؤسسه آموزش‌عالی غیرانتفاعی آل طه، تهران، ایران | ||
| 3پژوهشگاه رویان، پژوهشکده زیست‌شناسی و فناوری سلول‌های بنیادی جهاد دانشگاهی، مرکز تحقیقات علـوم سـلولی، گـروه فنـاوری نـانو و زیست مواد، تهران، ایران | ||
| چکیده | ||
| استخوان سخت‌ترین و یکی از مهم‌ترین بافت‌های بدن است. در صورت وقوع آسیب‌های استخوانی، درمان‌های فعلی باعث ترمیم و بازسازی کامل استخوان نمی‌شوند. به همین دلیل استراتژی‌های مهندسی بافت مبتنی بر سلول و به‌ویژه (Mesenchymal Stem Cells: MSCs) موردتوجه قرار گرفته است. یکی از این بسترها، هیدروژل‌های مشتق‌شده از بافت سلول‌زدایی‌شده هستند. در این پروژه، از هیدروژل مشتق‌شده از ماهیچه اسکلتی به نام میوژل (Myogel) استفاده شد. در این مطالعه تأثیر میوژل بر زنده‌مانی (روش MTT)، تکثیر (ترسیم منحنی رشد و محاسبه زمان دو برابرشدن جمعیت سلولی)، پروفایل چرخه سلولی (روش فلوسایتومتری) و مهاجرت سلول‌ها (روش ایجاد خراش) موردبررسی قرار گرفت. نتایج MTT نشان داد، زنده‌مانی MSCs در بستر میوژل با غلظت 2/0 میلی‌گرم بر میلی‌لیتر از زنده‌مانی MSCs در بستر ژلاتین با غلظت 1/0 میلی‌گرم بر میلی‌لیتر بیش‌تر و زنده‌مانی MSCs بستر ژلاتین از زنده‌مانی MSCs مربوط به کنترل بیش‌تر بود. بستر میوژل، تکثیر و مهاجرت سلول‌ها را افزایش و زمان دو برابرشدن جمعیت MSCs را کاهش داد. بررسی پروفایل چرخه سلولی نشان داد، درصد بالایی از سلول‌های کشت‌شده بر روی میوژل در مرحله G1 و S چرخه‌ی سلولی بودند که نشان‌دهنده افزایش سرعت تقسیم سلول‌ها توسط ژلاتین و در درجه بعد میوژل می‌باشد. بنابراین میوژل می‌تواند به‌عنوان بستری مناسب در مهندسی بافت مورداستفاده قرار گیرد. | ||
| کلیدواژه‌ها | ||
| میوژل؛ سلول بنیادی مزانشیمی؛ زنده‌مانی؛ چرخه سلولی | ||
| عنوان مقاله [English] | ||
| Investigating the biological activities of mesenchymal stem cells cultured on skeletal muscle-derived coating substrate | ||
| نویسندگان [English] | ||
| Faeze Zarean1؛ Somayeh Arabzadeh2؛ Sarah Rajabi3؛ Mahmood Talkhabi1؛ | ||
| 1Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran | ||
| 2Department of Biology, Faculty of Basic Science, Ale Taha Institute of Higher Education, Tehran, Iran | ||
| 3Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran | ||
| چکیده [English] | ||
| Bone is the hardest and one of the most important tissues in the body. In case of bone damage, the current treatments do not completely repair and regenerate the bone. For this reason, cell-based tissue engineering strategies, especially Mesenchymal Stem Cells (MSCs), have received attention. MSCs have the ability to self-renew and differentiate into different cell types, including bone cells, cartilage cells, and fat cells, among others. They are found in various tissues throughout the body, including bone marrow, adipose tissue, and umbilical cord tissue. Today, MSCs are a valuable resource for regenerative medicine and tissue engineering applications. In addition to the cells, scaffolds are another essential element of tissue engineering. One of these scaffolds is decellularized tissue-derived hydrogels, which are three-dimensional network of hydrophilic polymer chains that can absorb and retain a significant amount of water. In tissue engineering, they mimic the natural extracellular matrix of tissues, providing a suitable environment for cells to attach, proliferate, and differentiate. In the current study, we aimed to investigate the effects of decellularized skeletal muscle-derived hydrogel, known as Myogel, on bone marrow-derived MSCs biological behaviors, including proliferation, viability and migration. In this study, MSCs were isolated from tibia and femur of adult Wistar rats. MSCs were cultured in a complete medium (a-MEM containing 15% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Pen/Strep)). The identity of cells was determined by morphology (using inverted microscope) and expression of specific CD markers (using Flowcytometry). Skeletal muscle was decellularized and accuracy of decellularization was evaluated using special staining. Then Myogel was prepared from digested decellularized skeletal muscle. Here, Myogel substrate was used as the control group, gelatin substrate as the positive control, and un-coated plates as the negative control. The effect of Myogel on survival (MTT method), proliferation (drawing the growth curve and calculating the doubling time of the cell population), cell cycle profile (flow cytometry method), and cell migration (scratch method) were investigated. The MTT test showed that the survival of MSCs in Myogel substrate with a concentration of 0.2 mg/ml was higher than the survival of MSCs in gelatin substrate with a concentration of 0.1 mg/ml and the survival of MSCs in gelatin was higher than the survival of control MSCs. Myogel substrate increased the proliferation and migration of cells and decreased the doubling time of MSCs population. Examining the cell cycle profile showed that a high percentage of cells cultured on Myogel were in the G1 and S phase of the cell cycle, indicating an increase in cell division speed by gelatin and, in the next degree, by Myogel. Therefore, Myogel can be used as a suitable substrate to increase the proliferative and migratory potential of MSCs, which are an important factor in tissue engineering. | ||
| کلیدواژه‌ها [English] | ||
| Myogel, Mesenchymal Stem Cell, Viability, Cell cycle, Migration | ||
| مراجع | ||
|
Agmon, G., & Christman, K. L. (2016). Controlling stem cell behavior with decellularized extracellular matrix scaffolds. Current Opinion in Solid State and Materials Science, 20(4), 193-201.
Ahmadi, B. M., Noori, A., Ashtiani, M. K., Rajabi, S., & Talkhabi, M. (2023). 5-Azacytidine incorporated skeletal muscle-derived hydrogel promotes rat skeletal muscle regeneration. Cells & Development, 173, 203826.
Caplan, A. (2009). Why are MSCs therapeutic? New data: new insight. The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland, 217(2), 318-324.
Chen, X. D., Dusevich, V., Feng, J. Q., Manolagas, S. C., & Jilka, R. L. (2007). Extracellular matrix made by bone marrow cells facilitates expansion of marrow‐derived mesenchymal progenitor cells and prevents their differentiation into osteoblasts. Journal of bone and mineral research, 22(12), 1943-1956.
DeQuach, J. A., Mezzano, V., Miglani, A., Lange, S., Keller, G. M., Sheikh, F., & Christman, K. L. (2010). Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture. PloS one, 5(9), e13039.
Ding, D.-C., Shyu, W.-C., & Lin, S.-Z. (2011). Mesenchymal stem cells. Cell transplantation, 20(1), 5-14.
Dominici, M., Le Blanc, K., Mueller, I., Slaper-Cortenbach, I., Marini, F., Krause, D., Deans, R., Keating, A., Prockop, D., & Horwitz, E. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8(4), 315-317.
Duan, Y., Liu, Z., O’Neill, J., Wan, L. Q., Freytes, D. O., & Vunjak-Novakovic, G. (2011). Hybrid gel composed of native heart matrix and collagen induces cardiac differentiation of human embryonic stem cells without supplemental growth factors. Journal of cardiovascular translational research, 4, 605-615.
Fuoco, C., Sangalli, E., Vono, R., Testa, S., Sacchetti, B., Latronico, M. V., Bernardini, S., Madeddu, P., Cesareni, G., & Seliktar, D. (2014). 3D hydrogel environment rejuvenates aged pericytes for skeletal muscle tissue engineering. Frontiers in physiology, 5, 92444.
Ghiyasvand, F., Arabzadeh, S., & Talkhabi, M. (2023). Investigating the Effect of Ascorbic Acid on the Viability of Mesenchymal Stem Cell Cultured on the Skeletal Muscle Decellularized Scaffold. Experimental animal Biology, 12(1), 57-66.
Guilak, F., Cohen, D. M., Estes, B. T., Gimble, J. M., Liedtke, W., & Chen, C. S. (2009). Control of stem cell fate by physical interactions with the extracellular matrix. Cell stem cell, 5(1), 17-26.
Huang, S., Xu, L., Sun, Y., Wu, T., Wang, K., & Li, G. (2015). An improved protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Journal of orthopaedic translation, 3(1), 26-33.
Jensen, T., Roszell, B., Zang, F., Girard, E., Matson, A., Thrall, R., Jaworski, D. M., Hatton, C., Weiss, D. J., & Finck, C. (2012). A rapid lung de-cellularization protocol supports embryonic stem cell differentiation in vitro and following implantation. Tissue Engineering Part C: Methods, 18(8), 632-646.
Khanban, H., Fattahi, E., & Talkhabi, M. (2019). In vivo administration of G9a inhibitor A366 decreases osteogenic potential of bone marrow-derived mesenchymal stem cells. Excli journal, 18, 300.
Lee, H., Ju, Y. M., Kim, I., Elsangeedy, E., Lee, J. H., Yoo, J. J., Atala, A., & Lee, S. J. (2020). A novel decellularized skeletal muscle-derived ECM scaffolding system for in situ muscle regeneration. Methods, 171, 77-85.
Lee, W., Lee, V., Polio, S., Keegan, P., Lee, J. H., Fischer, K., Park, J. K., & Yoo, S. S. (2010). On‐demand three‐dimensional freeform fabrication of multi‐layered hydrogel scaffold with fluidic channels. Biotechnology and bioengineering, 105(6), 1178-1186.
Lin, H., Yang, G., Tan, J., & Tuan, R. S. (2012). Influence of decellularized matrix derived from human mesenchymal stem cells on their proliferation, migration and multi-lineage differentiation potential. Biomaterials, 33(18), 4480-4489.
Ohishi, M., & Schipani, E. (2010). Bone marrow mesenchymal stem cells. Journal of cellular biochemistry, 109(2), 277-282.
Porter, J. R., Ruckh, T. T., & Popat, K. C. (2009). Bone tissue engineering: a review in bone biomimetics and drug delivery strategies. Biotechnology progress, 25(6), 1539-1560.
Rather, H. A., Jhala, D., & Vasita, R. (2019). Dual functional approaches for osteogenesis coupled angiogenesis in bone tissue engineering. Materials Science and Engineering: C, 103, 109761.
Re, F., Sartore, L., Moulisova, V., Cantini, M., Almici, C., Bianchetti, A., Chinello, C., Dey, K., Agnelli, S., & Manferdini, C. (2019). 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation. Journal of tissue engineering, 10, 2041731419845852.
Wang, S., Qu, X., & Zhao, R. C. (2012). Clinical applications of mesenchymal stem cells. Journal of hematology & oncology, 5(1), 1-9.
Xiong, X., Yang, X., Dai, H., Feng, G., Zhang, Y., Zhou, J., & Zhou, W. (2019). Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells. Stem cell research & therapy, 10(1), 1-16.
Xuan, Z., Peng, Q., Larsen, T., Gurevich, L., de Claville Christiansen, J., Zachar, V., & Pennisi, C. P. (2023). Tailoring hydrogel composition and stiffness to control smooth muscle cell differentiation in bioprinted constructs. Tissue engineering and regenerative medicine, 20(2), 199-212.
Zhang, Y., He, Y., Bharadwaj, S., Hammam, N., Carnagey, K., Myers, R., Atala, A., & Van Dyke, M. (2009). Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype. Biomaterials, 30(23-24), 4021-4028.
Zhang, Z., Luo, X., Xu, H., Wang, L., Jin, X., Chen, R., Ren, X., Lu, Y., Fu, M., & Huang, Y. (2015). Bone marrow stromal cell‐derived extracellular matrix promotes osteogenesis of adipose‐derived stem cells. Cell biology international, 39(3), 291-299.
Zhu, J., & Marchant, R. E. (2011). Design properties of hydrogel tissue-engineering scaffolds. Expert review of medical devices, 8(5), 607-626. | ||
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