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بررسی پلیمورفیسم rs2016520 موجود در ژن PPARD و ارتباط آن با ﺳﻄﺢ لیپوپروتئین با چگالی پایین (LDL) در سرم خون جمعیت ایرانی | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 12، شماره 3 - شماره پیاپی 47، اسفند 1402، صفحه 29-39 اصل مقاله (1.17 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2024.69812.1931 | ||
| نویسندگان | ||
| هانیه حیدرزاده1؛ ویدا حجتی* 1؛ فرید ابن رسولی2 | ||
| 1گروه زیستشناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران | ||
| 2گروه زیستشناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| چربی خون یک مشکل جهانی و رو به افزایش در همه کشورها از جمله ایران و یکی از تهدیدهای مهم برای سلامت جامعه میباشد. چربی خون بالا بهعنوان یک مشکل چند عاملی تحت تأثیر مجموعه عوامل مختلف، بهویژه عوامل متابولیکی و ژنتیکی قرار دارد. هدف از این مطالعه، بررسی پلیمورفیسم rs2016520 در ژن PPARD و ارتباط آن با ﺳﻄﺢ لیپوپروتئین با چگالی پایین (LDL) میباشد. خونگیری از 137 فرد شامل 83 زن و 54 مرد ایرانی شامل 79 فرد سالم یا شاهد (LDL نرمال) (7/57 درصد) و 58 فرد با LDL بالاتر از 130 (3/42 درصد) در محدوده سنی 18 تا 60 سال با گرفتن رضایتنامه، انجام شد.DNA توسط کیت آماده استخراج شد. غلظت و کیفیتDNA جداشده بهوسیله دستگاه اسپکتروفتومتری و ژل آگارز سنجیده شد. شناسایی ژنوتیپها با روش PCR-RFLP انجام شد. میزان 6 درصد فراوانی ژنوتیپها در گروه باLDL نرمال: 8/3 درصد برای CC، 7/36 درصد برای CT و 5/59 درصد برای ژنوتیپ TT و در گروه باLDL بالاتر از 130: 1/12 درصد برای CC، 9/37 درصد برای CT و 0/50 درصد برای ژنوتیپ TT بود. اختلاف فراوانی ژنوتیپها بین دو گروه با LDL نرمال و گروه با LDL بالاتر از 130 معنیدار نبود (05/0 < P). 56/2 درصد از نمونهها، BMI پایینتر از 25 و 8/43 درصد، BMI بالاتر از 25 داشتند. نتایج مطالعه حاضر ارتباط معنیداری میان پلیمورفیسم rs2016520 و سطح LDL نشان نداد. | ||
| کلیدواژهها | ||
| پلیمورفیسم؛ چربی خون؛ ژن PPARD؛ لیپوپروتئین با چگالی پایین؛ rs2016520 | ||
| عنوان مقاله [English] | ||
| Evaluation of rs2016520 Polymorphism in PPARD Gene and its Association with Serum Low Density Lipoprotein (LDL) in Iranian Population | ||
| نویسندگان [English] | ||
| Hanieyh Heydarzadeh1؛ Vida Hojati1؛ Farid Ebnerasuly2 | ||
| 1Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran | ||
| 2Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Blood fat is a global and increasing problem in all countries, including Iran, and one of the most important threats to society's health. High blood fat as a multifactorial problem is under the influence of various factors, especially metabolic and genetic factors. The aim of this study is to investigate the rs2016520 polymorphism in the PPARD gene and its relationship with the level of low-density lipoprotein (LDL). Blood sampling from 137 people, including 83 Iranian women and 54 men, including 79 healthy people or controls (normal LDL) (57.7%) and 58 people with LDL above 130 (42.3%) in the age range of 18 to 60 years. It was done after obtaining consent. DNA was extracted by a ready kit. The concentration and quality of isolated DNA were measured by spectrophotometry and agarose gel. Genotypes were identified by PCR-RFLP method. 6% frequency of genotypes in the group with normal LDL: 3.8% for CC, 36.7% for CT and 59.5% for TT genotype and in the group with LDL higher than 130: 12.1% for CC, 37.9% for CT and it was 0.50% for TT genotype. The difference in the frequency of genotypes between the two groups with normal LDL and the group with LDL above 130 was not significant (p > 0.05). 2.56% of the samples had a BMI lower than 25 and 43.8% had a BMI higher than 25. The results of the present study did not show a significant relationship between rs2016520 polymorphism and LDL level. | ||
| کلیدواژهها [English] | ||
| Polymorphism, Blood lipids, PPARD gene, Low density lipoprotein, rs2016520 | ||
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