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شناسایی و تجزیه و تحلیل miRNAs در گونه‌های مختلف آویشن (Thymus) | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| دوره 13، شماره 1 - شماره پیاپی 43، مهر 1402، صفحه 1-10 اصل مقاله (489.18 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2023.67947.1908 | ||
| نویسندگان | ||
| ابوذر سورنی* 1؛ سپهر مرآتیان اصفهانی2؛ بدرالدین ابراهیم سید طباطبایی1 | ||
| 1گروه زیست فناوری، دانشکده کشاورزی، دانشگاه صنعتی اصفهان | ||
| 2گروه زیست فناوری، دانشکده کشاورزی ، صنعتی اصفهان | ||
| چکیده | ||
| امروزه گیاهان دارویی به دلیل متابولیت‌های مؤثر آن‌ها | ||
| کلیدواژه‌ها | ||
| آویشن؛ NGS؛ ترپن‌ها | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| Identification and characterization of miRNAs in various Thymus species | ||
| نویسندگان [English] | ||
| Aboozar Soorni1؛ Sepehr Meratian Esfahani2؛ Badraldin Ebrahim Sayed-Tabatabaei1 | ||
| 1Department of Biotechnology, College of Agriculture, Isfahan University of Technology | ||
| 2Department of Biotechnology, College of Agriculture, Isfahan University of Technology | ||
| چکیده [English] | ||
| Today, medicinal plants have attracted the attention of many individuals because of their secondary metabolites in the treatment of many diseases. One of these plants is called thyme, which contains a wide range of secondary metabolites. These include terpenes, flavonoids and antioxidants. Various techniques and methods have been developed to increase and produce these materials. In classical methods, environmental factors are changed to produce the most effective substance in medicinal plants, but in newer approaches that are based on plant genetics, higher yields are observed. One of these approaches is the use of miRNAs. miRNAs are a class of small RNAs that encode and encode non-coding proteins consisting of about 17-24 nucleotides. miRNAs. They control gene expression after transcription by mRNA analysis or inhibition of their translation, and play a variety of roles in biological and metabolic processes in plants and animals. There are several methods for identifying miRNAs, one of the simplest and least expensive of which is the bioinformatics detection method. In this study, in order to identify distinct miRNA in thyme, a study based on homology search between transcript information of thyme and miRNA was performed. First, transcriptomic information of thyme was refined and then blast was performed against all known miRNAs in the miRBase database, and finally 64 distinct candidate miRNAs were identified in different species of thyme. The identified target genes were analyzed using different software. In conclusion 14 miRNAs were observed in the synthetic pathways of terpenes. | ||
| کلیدواژه‌ها [English] | ||
| Thyme, NGS, Terpenes | ||
| مراجع | ||
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