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مطالعه رفتاری و الکتروفیزیولوژی روند ترمیم عصب سیاتیک قطع‌شده در موش صحرایی نر نژاد ویستار به‌کمک سلول‌های استرومایی بند ناف کشت داده‌شده بر داربست نانو الیاف Plga | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 13، شماره 1 - شماره پیاپی 49، شهریور 1403، صفحه 69-80 اصل مقاله (1.5 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2024.71486.1954 | ||
| نویسندگان | ||
| نسیم حیاتی* 1؛ امیرحسین فضلعلی1؛ غلامرضا کاکا2؛ کاظم پریور1 | ||
| 1گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد علوم تحقیقات، تهران، ایران | ||
| 2دانشگاه علوم پزشکی بقیه الله، تهران، ایران | ||
| چکیده | ||
| سابقه هدف: این مطالعه اثر درمانی سلول‌های خون بند ناف بر داربست غشا PLGA بر ترمیم عصب سیاتیک قطع‌شده موش صحرایی نر نژاد ویستار توسط بررسی رفتاری، الکتروفیزیولوژی ارزیابی شد. مواد و روش‌ها: موش‌های صحرایی نر در پنج گروه هفت‌تایی تقسیم شدند.که شامل گروه سالم؛ گروه دوم گروهی که عصب سیاتیک قطع‌شده و غشا PLGA در محل ضایعه بسته شد؛ گروه سوم فقط تزریق سلول‌های خون بند ناف در محل آسیب انجام شد گروه چهارم سلول‌های خون بند ناف بر داربست غشا PLGA در محل قطع عصب تزریق شد و گروه کنترل که عصب سیاتیک بدون مداخله درمانی قطع شد. میزان بهبودی به‌وسیله فعالیت حسی حرکتی عصب سیاتیک، مطالعات الکتروفیزیولوژی ارزیابی گردید. یافته‌ها: ارزیابی حرکتی عصب سیاتیک، گروه کنترل بازگشتِ حالت طبیعی در هفته هشتم مشاهده نشد، گروه سلول درمانی بر داربست غشا PLGA هفته هشتم ترمیم شد. میزان AMP هفته هشتم بعد از ترمیمِ گروه سلول درمانی با شیب ملایم نشانه روند بهبودی گروه سلول درمانی است. شمارش تعداد رشته‌های عصبی در سطحی برابر 1000 میکرومتر مربع، تعداد رشته‌های عصبی گروه‌های سلول درمانی هفته هشتم بعد از ترمیم، مقایسه با گروه کنترل و گروه غشا PLGA افزایش داشت. پایان هفته هشتم شاخص فعالیت حسی عصب سیاتیک (تست Hot Plate)، روند ترمیم گروه سلول درمانی بر داربست غشا PLGA به سایر گروه‌ها مشهودتر بود. نتیجه‌گیری: پیوند سلول خون بند ناف سبب ترمیم عصب سیاتیک شده و داربست غشا PLGA همراه سلول‌های خون بند ناف موجب تسریع ترمیم عصب سیاتیک می‌شود. | ||
| کلیدواژه‌ها | ||
| سلول‌های خون بند ناف؛ غشا PLGA؛ عصب سیاتیک؛ موش صحرایی | ||
| عنوان مقاله [English] | ||
| Behavioral and electrophysiological study of the healing process of severed sciatic nerve in male Wistar rats with the help of umbilical cord stromal cells cultured on Plga nanofiber scaffolds | ||
| نویسندگان [English] | ||
| Nasim Hayati1؛ Amirhosein Fazlali1؛ Gholamreza Kaka2؛ Kazem Parivar1 | ||
| 1Department of Cell Biology, Faculty of Basic Sciences, Islamic Azad University, Research Branch, Tehran, Iran | ||
| 2Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| چکیده [English] | ||
| Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies. Materials and Methods: After cutting the sciatic nerve, male rats were divided into 4 groups of 7 Healthy rats, sciatic nerve cut rats without therapeutic intervention, rats with umbilical cord blood cells on PLGA membrane scaffold at the nerve cut site, rats were treated with umbilical cord blood cell injection at the injury site. The degree of recovery was evaluated by the sensorimotor activity of the sciatic nerve, electrophysiological studies. Findings: motor evaluation of the sciatic nerve, the control group was not observed to return to the normal state in the eighth week, the cell therapy group was repaired on the PLGA membrane scaffold in the eighth week. The level of AMP in the 8th week after recovery of the cell therapy group with a gentle slope is a sign of the healing process of the cell therapy group. Counting the number of nerve fibers in a surface equal to 1000 square meters, the number of nerve fibers in the cell therapy groups increased after the eighth week of repair, compared to the control group and the PLGA membrane group. At the end of the eighth week, the sensory activity index of the sciatic nerve (Hot Plate test), the healing process of the cell therapy group on the PLGA membrane scaffold was more evident than the other groups. Conclusion: Umbilical cord blood cell transplantation causes sciatic nerve repair and PLGA membrane scaffold with cord blood cells accelerates sciatic nerve repair. | ||
| کلیدواژه‌ها [English] | ||
| Umbilical cord blood cells, PLGA membrane, sciatic nerve | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 25 تعداد دریافت فایل اصل مقاله: 28 |
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