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Barekzahi, Neda, Akbarzadeh-T, Niloufar. (1403). Fluorescence Studies and Electronic Absorption Spectroscopic Investigation of the Interaction between the Schiff base Nano-Complex of Zn (II) with FS-DNA. فصلنامه علمی زیست فناوری گیاهان زراعی, 11(2), 104-113. doi: 10.30473/ijac.2025.73057.1313
Neda Barekzahi; Niloufar Akbarzadeh-T. "Fluorescence Studies and Electronic Absorption Spectroscopic Investigation of the Interaction between the Schiff base Nano-Complex of Zn (II) with FS-DNA". فصلنامه علمی زیست فناوری گیاهان زراعی, 11, 2, 1403, 104-113. doi: 10.30473/ijac.2025.73057.1313
Barekzahi, Neda, Akbarzadeh-T, Niloufar. (1403). 'Fluorescence Studies and Electronic Absorption Spectroscopic Investigation of the Interaction between the Schiff base Nano-Complex of Zn (II) with FS-DNA', فصلنامه علمی زیست فناوری گیاهان زراعی, 11(2), pp. 104-113. doi: 10.30473/ijac.2025.73057.1313
Barekzahi, Neda, Akbarzadeh-T, Niloufar. Fluorescence Studies and Electronic Absorption Spectroscopic Investigation of the Interaction between the Schiff base Nano-Complex of Zn (II) with FS-DNA. فصلنامه علمی زیست فناوری گیاهان زراعی, 1403; 11(2): 104-113. doi: 10.30473/ijac.2025.73057.1313

Fluorescence Studies and Electronic Absorption Spectroscopic Investigation of the Interaction between the Schiff base Nano-Complex of Zn (II) with FS-DNA

Iranian Journal of Analytical Chemistry
دوره 11، شماره 2 - شماره پیاپی 22، آذر 2024، صفحه 104-113    اصل مقاله (897.31 K)
نوع مقاله: Full research article
شناسه دیجیتال (DOI): 10.30473/ijac.2025.73057.1313
نویسندگان
Neda Barekzahi؛ Niloufar Akbarzadeh-T*
Department of Chemistry, University of Sistan and Baluchestan, P.O. Box 98135-674, Zahedan, Iran
چکیده
In this project, in the first part, the Schiff base ligand (3-nitrobenzaldehyde + 2-aminothiophenol = H2L) was prepared. Then a nano-complex with the formula [Zn(HL)2Cl2] was synthesized using sonochemical method. Zn-nano-complex was identified by FT-IR, UV-Vis, 1H-NMR and Emission Scanning Electron Microscope (SEM). The spectroscopic results showed that the H2L ligand was coordinated to the metal through the N atom. The FT-IR spectrum of Zn-nano-complex showed the vibrational banb of the immunity group (C = N) at 1680-1600 cm-1. The studies obtained from the UV-Vis spectrum confirm n → π* and π→π* intra-ligand transitions. FS-DNA (salmon sperm DNA) and Zn-nano-complex were examined using UV-Vis spectroscopy, fluorescence spectroscopy, and gel electrophoresis. The Stern–Volmer equation was used to calculate the binding constant (Kb) and apparent bimolecular quenching constant (kq) for FS-DNA. Van der Waals forces and hydrogen bonds are important in the interaction between the DNA and the Zn (II) complex, according to an analysis of thermodynamic parameters (∆H°, ΔS° and ΔG°). The interaction mechanism was determined to be the groove-binding mode. These results highlight the complex's complicated effects on DNA structure and the need to use a variety of analytical techniques in order to fully investigate metal-DNA interactions.
کلیدواژه‌ها
Schiff Base؛ FS-DNA؛ Van Der Waals Interactions؛ Gel Electrophoresis
عنوان مقاله [English]
مطالعات فلورسانس و بررسی طیف‌سنجی جذب الکترونیکی برهم‌کنش بین نانو کمپلکس شیف باز روی (II) با FS-DNA
نویسندگان [English]
ندا بارکزایی؛ نیلوفر نیلوفر اکبرزاده تربتی
گروه شیمی، دانشگاه سیستان و بلوچستان، زاهدان، ایران
چکیده [English]
در این پروژه در قسمت اول لیگاند پایه شیف (3-nitrobenzaldehyde + 2-aminothiophenol = H2L) تهیه شد. سپس یک نانو کمپلکس با فرمول [Zn(HL)2Cl2] (a) با استفاده از روش سونوشیمیایی سنتز شد. [Zn(HL)2Cl2] (a) توسط FT-IR، UV-Vis، 1H-NMR و میکروسکوپ الکترونی روبشی (SEM) شناسایی شد. نتایج طیف‌سنجی نشان داد که لیگاند H2L از طریق اتم N به فلز کوردینه شده است. طیف FT-IR ترکیب (a) باند ارتعاشی گروه ایمنی (C = N) را در  cm-11680 -1600 نشان داد. مطالعات به دست آمده از طیف سنجی UV-Vis  انتقالات درون لیگاند *n → π و *π → π را تایید می کند. FS-DNA (DNA اسپرم ماهی قزل آلا) و کمپلکس (a) با استفاده از طیف‌سنجی UV-Vis، طیف‌سنجی فلورسانس و الکتروفورز ژل مورد بررسی قرار گرفتند. از معادله استرن-ولمر برای محاسبه ثابت اتصال (Kb) و ثابت خاموش شدن دو مولکولی ظاهری (kg) برای FS-DNA استفاده شد. بر اساس تجزیه و تحلیل پارامترهای ترمودینامیکی (H°∆، °ΔS و °ΔG) نیروهای واندروالس و پیوندهای هیدروژنی در برهمکنش بین DNA و کمپلکس روی (II) مهم هستند. مکانیسم برهمکش به حالت اتصال شیاری تعیین شد. این نتایج تأثیرات پیچیده این مجموعه را بر ساختار DNA و نیاز به استفاده از انواع تکنیک های تحلیلی به منظور بررسی کامل برهمکنش های فلز-DNA را برجسته می کند.
کلیدواژه‌ها [English]
شیف باز, DNA اسپرم ماهی, برهمکنش واندروالس, الکتروفورز ژل
مراجع
  • H. Abdel-Rahman, R. M. El-Khatib, L. A. E. Nassr, and A. M. Abu-Dief, DNA Binding Ability Mode, Spectroscopic Studies, Hydrophobicity, and in Vitro Antibacterial Evaluation of Some New Fe(II) Complexes Bearing ONO Donor Amino Acid Schiff Bases, Arab. J. Chem. 10 (2017) S1834-S1846.
  • Kargar, M. Fallah-Mehrjardi, R. Behjatmanesh-Ardakani, Kh. Sh. Munawar, M. Bahadorie, and M. Moghadam, Synthesis, spectral characterization, and theoretical investigation of Pd(II) complex incorporating unsymmetrical tetradentate Schiff base ligand and its application in Suzuki–Miyaura cross-coupling reaction, Inorg. Chem. Res. 6 (2022) 76-83.
  • Asadollahi, H. Golchoubian, and A. Shirvan, Mononuclear Nickel(II) and Zinc(II) Complexes with N-(2-propanamide)-2-picolylamine Ligand: Synthesis, Chromotropism Properties and DFT Calculations, Inorg. Chem. Res. 6 (2022) 146-154.
  • Rezaeivala, Synthesis, Characterization and Theoretical Studies of a New Macroacyclic SchiffBase Ligand Containing Piperazine Moiety and Related Mn(II), Cu(II), Ni(II) and Cd(II) Complexes, Inorg. Chem. Res. 1 (2017) 85-92.
  • Golbedaghi, A. Mohammadzaheri, M. Liyaghati-Delshad, N. Ajami, and M. Mahdavi Lasibi, Cu(II) Complexes of a Tripodal Tetraamine Incorporating a Pendant Arm and Related Schiff Base Ligand; X-ray Crystal Structure and Spectral StudiesInorg. Chem. Res. 7 (2023) 22-26.
  • Sahraei, H. Kargar, M. Hakimi and M. N. Tahir, Distorted square-antiprism geometry of new zirconium (IV) Schiff base complexes: Synthesis, spectral characterization, crystal structure and investigation of biological properties, J. Mol. Struct. 1149 (2017) 576-584.
  • Zamanpour, M. Asadi and G. Absalan, New Tetraaza Schiff Base Ligands and Their Complexes: Synthesis, Characterization and Thermodynamic Studies, Inorg. Chem. Res. 1 (2016) 59-68.
  • Kargar, M. Fallah-Mehrjardi, R, Behjatmanesh-Ardakani and Kh. Sh. Munawar, New Copper (II) Complexes with Benzimidazole and Benzoxazole Heterocyclic Ligands: Synthesis, Spectral Characterization, FMO, MEP, NBO, and DFT Study, Inorg. Chem. Res. 6 (2022) 48-57.
  • Joseph, K. Nagashri, and G. Ayisha Bibin Rani, Synthesis, characterization an antimicrobial activities of copper complexes derived from 4-aminoantipyrine derivatives Source Information, J. Saudi Chem. Soc. 17 (2013) 285-294.
  • Ö. Güngör, M. Çeşme, M. Emin Çınar, and A.  Gölcü, The new metal-based compound from anticancer drug cytarabine: Spectral, electrochemical, DNA-binding, antiproliferative effect and in silico studies,  Mol. Struct. 118 (2019) 532-543.
  • G. Mohamed, F. M. Elantabli, A. A. Abdel aziz, H. Moustafa, and S. M. El-Medani, J. Mol. Struct. 1176 (2019) 501-514.
  • Shafaatian, S. S. Mousavi, and S. Afshari, Synthesis, characterization, spectroscopic and theoretical studies of new zinc(II), copper(II) and nickel(II) complexes based on imine ligand containing 2-aminothiophenol moiety, J. Mol. Struct. 1123 (2016) 191-198.
  • Y. Nagesh, and B. H. M. Mruthyunjayaswamy, Synthesis, characterization and biological relevance of some metal (II) complexes with oxygen, nitrogen and oxygen (ONO) donor Schiff base ligand derived from thiazole and 2-hydroxy-1-naphthaldehyde, J. Mol. Struct, 1085 (2015) 198-206.
  • Abbasi, M. Salehi, A. Khaleghian, and M. Kubicki, In vitro cytotoxic activity of a novel Schiff base ligand derived from 2-hydroxy-1-naphthaldehyde and its mononuclear metal complexes, J. Mol. Struct. 1173 (2018) 213-220.
  • Izadyar, and H. Mansouri-Torshizi, E. Dehghanian, S. Shahraki, Spectroscopy, docking and molecular dynamics studies on the interaction between cisand transpalladium-alanine complexes with calf-thymus DNA and antitumor activities, J. Coord. Chem. 76 (3-4) (2023) 519-542.
  • Kondori, N. Akbarzadeh-T, and C. Graiff, Synthesis, X-ray structural analysis, antibacterial and DNA-binding studies of a lanthanum bis-(5, 5′-dimethyl-2, 2′-bipyridine) complex, J. Iran. Chem. Soc. 16(9) (2019) 1827-1838.
  • Mudasir, K.Wijaya, E. T. Wahyuni, N. Yoshioka, and H. Inoue, Salt-dependent binding of iron(II) mixed-ligand complexes containing 1,10-phenanthroline and dipyrido[3,2-a:2′,3′-c]phenazine to calf thymus DNA, Biophys. Chem. 121(1) (2006) 44-50.
  • Gurumoorthy, D. Mahendiran, D. Prabhu, C. Arulvasu, and A. K. Rahman, Mixed-ligand copper (II) phenolate complexes: Synthesis, spectral characterization, phosphate-hydrolysis, antioxidant, DNA interaction and cytotoxic studies, J. Mol. Struct. 1080 (2015) 88-98
  • Fazli, N. Akbarzadeh-T, H. Beitollahi, M. Dusˇek, and V. Eigner, New Schiff base ligand N-(2-hydroxy-1-naphthylidene)- 2-methyl aniline and its nano-sized copper(II) complex: synthesis, characterization, crystal structure and application as an electrochemical sensor of 2- phenylphenol in the presence of 4-chlorophenol, J. Mater. Sci.: Mater. Electron., 32 (2021) 25118-25136.
  • Cohen, and H. Eisenberg, Viscosity and sedimentation study of sonicated DNA–proflavine complexes. Biopolymers, 8(1) (1969) 45-55.
  • Kargar, V. Torabi, A. Akbari, R. Behjatmanesh-Ardakani, and M. N. Tahi, Synthesis, characterization, crystal structure and DFT studies of a palladium (II) complex with an asymmetric Schiff base ligand, J. Mol. Struct. 1179 (2019) 732-738.
  • . Kargar, and R. Kia, Synthesis and Crystal Structures of Three New Hetero-binuclear Hg(II)-Cu(II) Schiff Base Complexes, Chem. Res. 1 (2017) 40-49.
  • Alizadeh, and V. Amani, Syntheses, crystal structures, and photoluminescence of three cadmium(II) coordination complexes based on bipyridine ligands with different positioned methyl substituents, J. Inorg. Chim. Acta. 443 (2016)151–159.
  • Kashanian, M. M. Khodaei, and P. Pakravan, Spectroscopic studies on the interaction of isatin with calf thymus DNA, DNA and Cell Biology. 29(10) (2010) 639-648.
  • Abdi, H. Hadadzadeh, M. Salimi, J. Simpson, and A. D. Khalaji, A mononuclear copper(II) complex based on the polypyridyl ligand 2,4,6-tris(2-pyridyl)-1,3,5-triazine (tptz), [Cu(tptz)2]2+: X-ray crystal structure, DNA binding and in vitrocell cytotoxicity. Polyhedron.44 (1) (2012)101-112.
  • R. Lakowicz, and G. Weber, Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale, J. Biochem. 12(21) (1973) 4171-4179.
  • Kumari, J. K. Maurya, U. K. Singh, A. B. Khan, M. Ali, P. Singh, and R. Patel, Spectroscopic and docking studies on the interaction between pyrrolidinium based ionic liquid and bovine serum albumin, Spectrochim. Acta. A Mol. Biomol. Spectrosc. 124 (2014) 349-356.
  • Belatik , S. Hotchandani,  J. Bariyanga, and H. A. Tajmir-Riahi, Binding sites of retinol and retinoic acid with serum albumins, Eur. J. Med. Chem. 48 (2021) 114-123.
  • Mansouri-Torshizi, S. Zareian-Jahromi, Kh. Abdi, and M. Saeidifar, Nonionic but water soluble,[Glycine-Pd-Alanine] and [Glycine-Pd-Valine] complexes. Their synthesis, characterization, antitumor activities and rich DNA/HSA interaction studies, J. Biomol. Struct. Dyn. 37(13) (2019) 3566-3582.
  • Saeidifar, H. Mansouri-Torshizi, Y. Palizdar, M. Eslami-Moghaddam, A. Divsalar, and A. A. Saboury, Synthesis, characterization, cytotoxicity and DNA binding studies of a novel anionic organopalladium (II) complex, Acta. Chimica.Slovenica. 61(1) (2014) 126–136.
  • Heydari, and H. Mansouri-Torshizi, Synthesis, characterization, cytotoxicity and DNA binding studies of a novel anionic organopalladium (II) complex, RSC Advances. 6(98) (2016) 96121-96137.
  • Mahaki, H. Tanzadehpanah, O. K. Abou-Zied, N. H. Moghadam, A. Bahmani, S. Salehzadeh, D. Dastan, and M. Saidijam, Cytotoxicity and antioxidant activity of Kamolonol acetate from Ferula pseudalliacea, and studying its interactions with calf thymus DNA (ct-DNA) and human serum albumin (HSA) by spectroscopic and molecular docking techniques, Process Biochem. 79 (2019) 203-213.
  • S. Lerman, Structural considerations in the interaction of DNA and acridines, J. Mol. Biol. 3(1) (1961) 18-IN14.
  • Moradi, M. Khorasani-Motlagh, A. R. Rezvani, and M. Noroozifar, Evaluation of DNA, BSA binding, and antimicrobial activity of new synthesized neodymium complex containing. 2-dimethyl 1-10-phenanthroline. J. Biomol. Struct. Dyn.36 (3) (2018) 779-794.
  • ]T. Kondori, O. Shahraki, N. Akbarzadeh-T, and Z. Aramesh-Boroujeni, Two Novel Bipyridine-based Cobalt (II) Complexes: Synthesis, Characterization, Molecular Docking, DNA-Binding and Biological Evaluation, Biomol. Struct. Dyn. 39(2) (2020) 1-27.
  • Grueso, G. Lopez-Perez, M. Castellano, and R. Prado-Gotor, Thermodynamic and structural study of phenanthroline derivative ruthenium complex/DNA interactions: probing partial intercalation and binding properties, J. Inorg. Biochem. 106(1) (2012) 1-9.
  • Zareian-Jahromi, and H. Mansouri-Torshizi, Synthesis, characterization, DNA and HSA binding studies of isomeric Pd (II) antitumor complexes using spectrophotometry techniques, J. Biomol. Struct. Dyn. 36(5) (2018) 1329-1350.
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