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Arvand, Majid, Pourhabib, Akram, Arvand, Bahar. (1403). Molecularly Imprinted Polymer-Based Electrochemical Sensor for the Specific Sorption and Detection of Folic Acid in Biological Matrices and Food Samples. فصلنامه علمی زیست فناوری گیاهان زراعی, 11(2), 163-172. doi: 10.30473/ijac.2025.74192.1317
Majid Arvand; Akram Pourhabib; Bahar Arvand. "Molecularly Imprinted Polymer-Based Electrochemical Sensor for the Specific Sorption and Detection of Folic Acid in Biological Matrices and Food Samples". فصلنامه علمی زیست فناوری گیاهان زراعی, 11, 2, 1403, 163-172. doi: 10.30473/ijac.2025.74192.1317
Arvand, Majid, Pourhabib, Akram, Arvand, Bahar. (1403). 'Molecularly Imprinted Polymer-Based Electrochemical Sensor for the Specific Sorption and Detection of Folic Acid in Biological Matrices and Food Samples', فصلنامه علمی زیست فناوری گیاهان زراعی, 11(2), pp. 163-172. doi: 10.30473/ijac.2025.74192.1317
Arvand, Majid, Pourhabib, Akram, Arvand, Bahar. Molecularly Imprinted Polymer-Based Electrochemical Sensor for the Specific Sorption and Detection of Folic Acid in Biological Matrices and Food Samples. فصلنامه علمی زیست فناوری گیاهان زراعی, 1403; 11(2): 163-172. doi: 10.30473/ijac.2025.74192.1317

Molecularly Imprinted Polymer-Based Electrochemical Sensor for the Specific Sorption and Detection of Folic Acid in Biological Matrices and Food Samples

Iranian Journal of Analytical Chemistry
دوره 11، شماره 2 - شماره پیاپی 22، آذر 2024، صفحه 163-172    اصل مقاله (695.65 K)
نوع مقاله: Full research article
شناسه دیجیتال (DOI): 10.30473/ijac.2025.74192.1317
نویسندگان
Majid Arvand* 1؛ Akram Pourhabib2؛ Bahar Arvand3
1Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914–41335, Rasht, Iran
2Education Organization of Guilan Province, Farhang Square, P.O. Box: 41849-83111, Rasht, Iran
3School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
چکیده
This study presents an innovative biomimetic sensor leveraging molecularly imprinted polymers (MIPs) for folic acid detection, combining advanced materials and electrochemical design for enhanced performance. The sensor employs methacrylic acid monomers polymerized onto a polymeric surface, integrated with graphite powder and paraffin oil to form a robust carbon paste electrode. Key strengths include its Nernstian response (19.2 mV decade–1) across a wide concentration range (5 × 10−9 to 1 × 10−3 mol L−1) and an impressively low detection limit of 1 × 10−9 mol L−1, surpassing many conventional methods. The design eliminates the need for additional reagents or complex instrumentation, prioritizing cost-effectiveness and simplicity. Novel aspects lie in the MIP-graphite-paraffin oil composite, which enhances stability and selectivity while enabling rapid sensor regeneration through surface polishing. The sensor demonstrates remarkable long-term stability and reproducibility, critical for real-world applications in pharmaceutical samples. Its ability to discern folic acid from interferents, validated in diverse matrices, underscores its practicality for clinical diagnostics and food quality control. By merging MIP specificity with electrochemical transduction, this work advances portable, high-sensitivity sensing platforms for routine analysis.
کلیدواژه‌ها
Folic Acid؛ Molecularly Imprinted Polymer؛ Electrochemistry؛ Potentiometric Transduction؛ Tablet؛ Serum
عنوان مقاله [English]
حسگر الکتروشیمیایی بر پایه پلیمر چاپ مولکولی برای جذب اختصاصی و آشکارسازی فولیک اسید در ماتریس های بیولوژیکی و نمونه های غذایی
نویسندگان [English]
مجید آروند1؛ اکرم پورحبیب2؛ بهار آروند3
1دانشکده شیمی، دانشگاه گیلان، رشت، ایران
2آموزش و پرورش استان گیلان، رشت، ایران
3دانشکده پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
چکیده [English]
این مطالعه یک حسگر زیست تقلیدی نوآورانه را ارائه می‌دهد که از پلیمرهای قالب مولکولی (MIPs) برای تشخیص فولیک اسید استفاده می‌کند و مواد پیشرفته و طراحی الکتروشیمیایی را برای عملکرد بهتر ترکیب می‌نماید. این حسگر از مونومرهای متاکریلیک اسید پلیمریزه شده روی یک سطح پلیمری، که با پودر گرافیت و روغن پارافین ادغام شده‌اند، برای تشکیل یک الکترود خمیر کربنی استفاده می‌کند. نقاط قوت کلیدی آن شامل پاسخ نرنستی (mV decade-119.2 ) در گستره غلظتی وسیع (9-10 × 5 تا 3-10 × 1 مول بر لیتر) و حد تشخیص بسیار پایین 9-10 × 1 مول بر لیتر است که از بسیاری از روش‌های مرسوم پیشی می‌گیرد. این طراحی نیاز به معرف‌های اضافی یا ابزار دقیق پیچیده را از بین می‌برد و مقرون به صرفه بودن و سادگی را در اولویت قرار می‌دهد. جنبه‌های بدیع در کامپوزیت MIP-گرافیت-روغن پارافین نهفته است که پایداری و گزینش‌پذیری را افزایش می‌دهد و در عین حال امکان بازسازی سریع حسگر را از طریق صیقل دادن سطح فراهم می‌کند. این حسگر پایداری و تکرارپذیری بلندمدت قابل توجهی را نشان می‌دهد که برای کاربردهای دنیای واقعی در نمونه‌های دارویی بسیار مهم است. توانایی آن در تشخیص فولیک اسید از گونه های تداخل‌کننده، که در ماتریس‌های متنوع اعتبارسنجی شد، بر کاربردی بودن آن برای تشخیص‌های بالینی و کنترل کیفیت مواد غذایی تأکید می‌کند. با ادغام ویژگی MIP با تبدیل الکتروشیمیایی، این کار بسترهای حسگری قابل حمل و با حساسیت بالا را برای آنالیزهای روتین ارائه می دهد.
 
کلیدواژه‌ها [English]
فولیک اسید, پلیمر چاپ مولکولی, الکتروشیمی, تبدیل سیگنال پتانسیومتری, قرص, سرم
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آمار
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