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شناسایی نشانگرهای SSR پیوسته با صفات مورفولوژیک در ژنوتیپ های لوبیا چشم بلبلی (Vigna unguiculata L. Walp) تحت تنش خشکی | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 2، دوره 5، شماره 4 - شماره پیاپی 14، شهریور 1395، صفحه 43-60 اصل مقاله (381.37 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| خسرو مفاخری1؛ محمدرضا بی همتا* 2؛ علیرضا عباسی3 | ||
| 1دانشجوی دکتری اصلاح نباتات، گروه به‌نژادی و بیوتکنولوژی، دانشکده کشاورزی دانشگاه تبریز، تبریز، ایران | ||
| 2استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه تهران، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 3دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه تهران، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| چکیده | ||
| به منظور شناسایی نشانگرهای پیوسته با صفات مرفوفنولوژیک در ژنوتیپ های لوبیا چشم بلبلی، از 22 جفت نشانگرهای ریزماهواره (SSR) استفاده شد. آغازگرها، 186 مکان در 32 ژنوتیپ لوبیا چشم بلبلی تولید کردند. میانگین تعداد مکان ها 45/8 مکان برای هر آغازگر و میزان اطلاعات چندشکلی (PIC) برای آغازگرها 445/0 از 25/0 (Vm25) تا 625/0 (Vm5) متغیر بود. به منظور بررسی فاصله ژنتیکی بین ژنوتیپ ها تجزیه خوشه ای برای داده های حاصل از نشانگرهای SSR براساس ضرایب تشابه دایس و جاکارد ژنوتیپ ها را در دو گروه اصلی قرار داد. به منظور شناسایی نشانگرهای مولکولی مثبت، تجزیه رگرسیون گام به گام بین داده های مولکولی به عنوان متغیرهای مستقل و صفات مورفولوژیک به عنوان متغیرهای وابسته در هر دو شرایط خشکی و نرمال انجام گرفت. نشانگرهای مرتبط با صفات عملکرد اقتصادی و بیولوژیک به ترتیب در شرایط نرمال Vm70 و Vm33، در خشکی Vm3 و Vm26 بودند. در تنش خشکی بیشترین تغییرات مربوط به صفات تعداد روز تا 50% گلدهی، تعداد روز تا 50% رسیدگی غلاف و طول غلاف (9/99درصد) به ترتیب توسط نشانگرهای Vm22، Vm31 و Vm26تبین شد و در شرایط نرمال بیشترین تغییرات مربوط به صفات ضخامت غلاف، عرض غلاف و تعداد روز تا 50 درصد گلدهی (9/99 درصد) توسط نشانگرهای Vm14، Vm34 و Vm34 تبین شدند. در هر دو شرایط تنش خشکی و نرمال اکثر آغازگرهای مورد استفاده روی صفات ارتباط نشان دادند، بنابراین می‌توان از این آغازگرها همراه با اطلاعات صفات مرفوفنولوژیک در اصلاح لوبیا چشم‌بلبلی جهت شناسایی ژنوتیپ‌های متحمل به تنش خشکی، مناسب برای تهیه جمعیت‌های نقشه‌یابی و تولید ارقام متحمل استفاده کرد. | ||
| کلیدواژه‌ها | ||
| لوبیا چشم‌بلبلی؛ نشانگرهای مثبت؛ صفات مورفولوژیک و نشانگرهای مولکولی | ||
| موضوعات | ||
| اصلاح نباتات مولکولی | ||
| عنوان مقاله [English] | ||
| Identification of SSR markers associated with morphological traits in cowpea (Vigna unguiculata L. Walp) genotypes under drought stress condition | ||
| نویسندگان [English] | ||
| Khosro Mafakheri1؛ Mohammad Reza Bihamta2؛ Ali Reza Abbasi3 | ||
| 1Ph.D Student, Department of Biotechnology and Plant Breeding, University of Tabriz, Tabriz, Iran. | ||
| 2Professor, Department of Agronomy and Plant Breeding, University of Tehran, Tehran, Iran | ||
| 3Associate Professor, Department of Agronomy and Plant Breeding, University of Tehran, Tehran, Iran. | ||
| چکیده [English] | ||
| In the current investigation, 22 SSR primer pairs were used to identify molecular markers associated with morphological traits in cowpea (Vigna unguiculata L.) based association analysis, markers produced 186 locations in 32 cowpea genotypes. 22 SSR primers pair amplified 8.45 for each primer location and the average polymorphism information content (PIC) ranged from 0.445 to 0.25 (Vm25) and 0.625 (Vm5) were varied, respectively. Cluster analysis based on Dice, Jaccard and Simple Maching similarity coefficients based on molecular traits revealed primarily two major groups. Stepwise regression analysis between molecular data as independent variables, and morphological data as dependent variables was performed to identify informative markers associated with the studied traits. SSR loci associated with economic and biological yield, in normal conditions and in drought stress condition were Vm70 and Vm33, and Vm3 and Vm26 respectively. In drought stress condition the maximum variation of number of days to 50% flowering, days to 50% maturity of pods and pod length (99.9%, respectively) was accounted by Vm22, Vm31 and Vm26 markers. While in the normal condition the maximum variation of traits sheath thickness, width of pod and days to 50% flowering (99.9%) was accounted by Vm14, Vm34 and Vm34 markers. In both normal and drought conditions most of the used SSR primers showed significant association with the studied traits, so we can use these markers along with morphological traits in breeding cowpea genotypes to identify drought tolerant and hardy cultivars suitable for the preparation and use mapping populations. | ||
| کلیدواژه‌ها [English] | ||
| Cowpea, Positive Markers, Morphological Traits, Molecular markers | ||
| مراجع | ||
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