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مطالعه in silico خانواده ژنی شبه استریکتوسیدین سینتاز (SSL) در برنج (Oryza sativa) | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 2، دوره 6، شماره 2 - شماره پیاپی 16، اسفند 1395، صفحه 13-29 اصل مقاله (491.37 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| امین عابدی1؛ رضا شیرزادیان خرم آباد* 2؛ محمد مهدی سوهانی3 | ||
| 1دانشجوی دکترای بیوتکنولوژی کشاورزی-گیاهی، دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران | ||
| 2استادیار گروه بیوتکنولوژی کشاورزی دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران | ||
| 3دانشیار گروه بیوتکنولوژی کشاورزی دانشکده علوم کشاورزی دانشگاه گیلان، رشت، ایران | ||
| چکیده | ||
| استریکتوسیدین سینتاز آنزیم کلیدی مسیر بیوسنتزی ایندول آلکالوئیدهای مونوترپنوئیدی است. پروتئین‌های داری دمین Str_synth در گیاهان، باکتر‌ی‌ها، حشرات و پستانداران شناسایی شده‌اند و به علت نامشخص بودن نقش کارکردی آن‌ها شبه استریکتوسیدین سینتاز نامیده می‌شوند. با تکمیل پروژه توالی یابی ژنوم آرابیدوپسیس و برنج، ژن‌های شبه استریکتوسیدین سینتاز در این گیاهان نیز شناسایی شد. با این حال اطلاعات کافی در مورد ساختار ژن، تاریخچه تکاملی، نحوه گسترش و نقش کارکردی خانواده ژنی SSL برنج وجود ندارد. در این مطالعه بر اساس آنالیزهای بیوانفورماتیکی 23 ژن SSL در ژنوم برنج شناسایی شد. آنالیز فیلوژنتیکی پروتئین‌های SSL برنج و آرابیدوپسیس مشخص کرد که این ژن‌ها در چهار گروه قرار می‌گیرند و مسیر تکاملی این خانواده در برنج و آرابیدوپسیس متفاوت است. ژن‌های OsSSL دارای صفر تا پنج اینترون بوده و در 10 کروموزوم از 12 کروموزوم برنج با تراکم متفاوت قرار داشته و مضاعف‌شدگی پشت سر هم عامل اصلی گسترش این خانواده ژنی است. آنالیز پیشبر نشان داد که چندین عنصر تنظیمی cis پاسخ به تنش‌ها و هورمون‌ها در ناحیه تنظیمی وجود دارد که نشان دهنده نقش این ژن‌ها در پاسخ به تنش‌ها می-باشد. آنالیز بیان ژن‌های OsSSL بر اساس داده‌های ریزآرایه نشان داد که تعداد اندکی از این ژن‌ها در بافت‌ها و نیز تنش‌های غیر زیستی بیان بالایی دارند. این مطالعه اولین گزارش در مورد خانواده ژنی SSL در برنج بوده و می‌تواند یک چهارچوب برای بررسی کارکردهای بیولوژیکی ژن‌های SSL در برنج و گیاهان دیگر فراهم می‌کند. | ||
| کلیدواژه‌ها | ||
| آنالیز بیان؛ آنالیز فیلوژنتیکی؛ خانواده ژنی؛ مضاعف‌شدگی؛ مطالعات In Silico | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| In silico study of strictosidine synthase like (SSL) gene family in rice (Oriza sativa) | ||
| نویسندگان [English] | ||
| Amin Abedi1؛ Reza Shirzadian-Khorramabad2؛ Mohamad mehdi Sohani3 | ||
| 1Ph.D student, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 3Associate Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| Strictosidine synthase is a key enzyme in the monoterpenoid indole alkaloids biosynthesis pathway. Proteins with Str_synth domain have been identified in plants, bacteria, insects and even mammalians and called Strictosidine synthase-like due to unknown functional roles. With the Arabidopsis and rice genome sequence completed, SSL genes were also identified in these plants. However, little is known about evolutionary path, gene structure, expansion and function of SSL family in rice. In this study, through bioinformatic analysis, a total of 23 SSL genes were identified in rice genome. A phylogenetic analysis of the SSL genes in rice and Arabidopsis clarified that these genes could be divided into four different groups and the evolutionary paths are different in rice and Arabidopsis. The OsSSL genes contained zero to five introns and were distributed across 10 out of 12 chromosomes at different densities and tandem duplication was a major cause in expanding this family. Promoter analysis showed the presence of several cis-regulatory elements related to stress and hormone response in regulatory region, indicating probable their role in stress response. Microarray-based expression analysis of OsSSL genes indicated that a few number of these genes were widely expressed in various tissues and also in response to some abiotic stresses. This study is the first report about SSL gene family in rice and provides a framework for further analysis of the biological functions of SSL genes in either rice or other crops. | ||
| کلیدواژه‌ها [English] | ||
| "Expression Analysis", "Gene Family", "in silico Study", "Phylogenetic Analysis" | ||
| مراجع | ||
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