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تجزیه QTL غلظت سدیم و پتاسیم و نسبت پتاسیم به سدیم در گندم تحت شرایط تنش شوری | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 5، دوره 5، شماره 4 - شماره پیاپی 16، اسفند 1395، صفحه 61-73 اصل مقاله (1.28 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| امین آزادی* 1؛ محسن مردی2؛ اسلام مجیدی3؛ سید ابوالقاسم محمدی4؛ فواد مرادی5 | ||
| 1گروه اصلاح نباتات، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2دانشیار، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| 3گروه اصلاح نباتات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 4گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران | ||
| 5استادیار، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| چکیده | ||
| تاکنون QTLهای زیادی برای عملکرد و اجزای آن در گندم نان در شرایط نرمال شناسایی شده است. به منظور شناسایی QTLهای مرتبط با غلظت سدیم و پتاسیم در گندم نان تحت شرایط تنش شوری، از جمعیت 186 لاین اینبرد نوترکیب حاصل از تلاقی دو والد سوپرهد 2 و روشن استفاده شد. اعمال تیمارهای تنش شوری با استفاده از سیستم هیدروپونیک و در داخل گلخانه صورت پذیرفت. شرایط نرمال (10 میلی‌مولار NaCl) و تنش شوری (150 میلی‌مولار NaCl) در نظر گرفته شد و اعمال تنش به صورت مرحله‌ای صورت پذیرفت. نقشه ژنتیکی شامل 428 نشانگر دارت و 23 نشانگر ریز ماهواره بود. سه QTL برای هر یک از صفات غلظت سدیم و پتاسیم و یک QTL برای نسبت پتاسیم به سدیم بر روی کروموزوم‌هایA 4، B2، B3، B7 وD2 با استفاده از روش مکان‌یابی فاصله‌ای مرکب شناسایی گردید. QNa.abrii-3B، با LOD برابر 9/5، در حدود 3/8 درصد از تغییرات واریانس فنوتیپی غلظت سدیم را در شرایط تنش شوری توجیه می‌کرد و نشانگر gwm247 لینکاژ شدید با این QTL نشان داد. همچنین دو QTL برای غلظت سدیم بر روی کروموزوم‌های B2 و D2 شناسایی شدند و QNa.abrii-2D حدود 2/9 درصد واریانس فنوتیپی این صفت را در شرایط تنش شوری توجیه می‌کرد. با ادامه تحقیقات در آینده، احتمال شناسایی QNa.abrii-2B و QNa.abrii-2D به عنوان گروه دو همیولوگی در گندم، در کنار ژن Nax1 وجود دارد. | ||
| کلیدواژه‌ها | ||
| QTL؛ غلظت سدیم؛ غلظت پتاسیم؛ گندم نان؛ تنش شوری | ||
| موضوعات | ||
| بیوتکنولوژی و تنش های زنده و غیرزنده | ||
| عنوان مقاله [English] | ||
| QTL Analysis for Sodium and Potassium Concentration and Potassium to Sodium Ratio in Wheat Under Salt-Stress Condition | ||
| نویسندگان [English] | ||
| Amin Azadi1؛ Mohsen Mardi2؛ Eslam Majidi Harvan3؛ Seyed Abolghasem Mohammadi4؛ Foad Moradi5 | ||
| 1Department of Plant Breeding, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran. | ||
| 2Associate Professor, Agriculture Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| 3Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 4Department of Agronomy & Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| 5Assistant Professor, Agriculture Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| So far, many quantitative trait loci (QTLs) have been detected for yield and its components in wheat under normal conditions. In order to identify QTLs associated with concentrations of sodium and potassium in bread wheat under salt stress conditions, a population consisted of 186 recombinant inbred lines from a cross between Roshan ×SuperHead#2 were evaluated. Salinity treatments were performed using a hydroponic system in a greenhouse. Normal conditions (10 mM NaCl) and salinity (150 mM NaCl) were considered and stress was conducted in stages. The molecular genetic map of the population consisted of 23 simple sequence repeat (SSR) and 428 diversity arrays technology (DArT) markers. Three QTLs for each of sodium and potassium concentration traits and a QTL for potassium to sodium ratio were detected on chromosomes 4A, 2B, 3B, 7B and 2D using composite interval mapping approach. The QNa.abrii-3B, with a LOD score of 5.9, explained 8.3 % of the phenotypic variation for sodium concentration under salt stress conditions and gwm247 marker showed a strong linkage with this QTL. In addition, two novel QTLs for sodium concentration were detected on chromosomes 2B and 2D and QNa.abrii-2D explained 9.2 % of the phenotypic variation for this trait under salt stress conditions. Proceed with future researches, there is probability of identifying QNa.abrii-2B and QNa.abrii-2D as two homoeologous group in wheat, beside Nax1 gene. | ||
| کلیدواژه‌ها [English] | ||
| QTL, sodium concentration, potassium concentration, bread wheat, Salt stress | ||
| مراجع | ||
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