پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 41 |
| تعداد شمارهها | 1,138 |
| تعداد مقالات | 9,757 |
| تعداد مشاهده مقاله | 17,852,330 |
| تعداد دریافت فایل اصل مقاله | 12,467,573 |
مطالعه پاسخ پروتئینی ریشه آفتابگردان به تنش خشکی با رهیافت پروتئومیکس | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 2، دوره 7، شماره 4 - شماره پیاپی 22، شهریور 1397، صفحه 15-26 اصل مقاله (514.56 K) | ||
| نوع مقاله: علمی پژوهشی | ||
| نویسندگان | ||
| مهدی غفاری* 1؛ سعادت شریفی2؛ غلامرضا بخشی خانیکی3 | ||
| 1موسسه تحقیقات، اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 2دانشآموخته کارشناسیارشد زیستشناسی، دانشگاه پیام نور، تهران | ||
| 3استاد گروه زیستشناسی، دانشگاه پیام نور، تهران | ||
| چکیده | ||
| برای درک مکانیسم مولکولی تحمل به خشکی در ارتباط با ریشه آفتابگردان، الگوی پروتئوم ریشه دو لاین حساس و متحمل به خشکی در شرایط آبیاری محدود و مطلوب بررسی شد. با استفاده از الکتروفورز دو بعدی و مقایسه شدت نسبی لکهها با آزمونt تعداد 12 لکه از 417 لکه پروتئینی در لاین حساس و 17 لکه از 467 لکه پروتئینی در لاین متحمل به طور معنیداری از تنش خشکی متأثر شد. لکههای پروتئینی با استفاده از طیفسنجی جرمی nano-LC MS/MS توسط جستجوگر مسکات با در نظر گرفتن حداقل 10% همپوشانی و امتیاز بالاتر از 80 در پایگاه NCBI شناسایی شدند. پروتئینهای سیتوپلاسمی و هستهای به ترتیب بیش از سایر پروتئینها از محدودیت آب متأثر شدند. سه لکه پروتئینی انولاز، گلیسرآلدئید تری فسفات دهیدروژناز و چالکون سینتاز با بیان متمایز در هر دو لاین حساس و متحمل شناسایی شد. کاهش شدت نسبی انولاز در لاین متحمل دلالت بر آسیب متابولیکی بود که میتواند منجر به کاهش فرایندهای پایین دست در اثر محدودیت ناشی از خشکی باشد. در مقابل افزایش شدت نسبی گلیسرآلدئید تریفسفات دهیدروژناز و چالکونسینتاز در لاین متحمل نشاندهنده توانایی این لاین در سمیتزدایی/ترمیم آسیب-های ناشی از تخریب اکسیداتیو و دفاع ضداکسیداتیو در شرایط تنش بود. افزایش شدت نسبی پروتئین شوکدمایی، دیهیدروفلاونول ردوکتاز، لیپو-اکسیژناز لینولئاتدانه، یوبیکوئیتین کربوکسیلترمینال هیدرولاز و جی پروتئین در لاین متحمل نشاندهنده اهمیت فرایندهای دفاعی، حفاظتی و ترارسانی برای کاهش آسیبهای ناشی از خشکی است. | ||
| کلیدواژهها | ||
| الکتروفورز دوبعدی؛ انولاز؛ پروتئین شوک دمایی؛ طیف سنجی جرمی | ||
| موضوعات | ||
| پروتئومیکس | ||
| عنوان مقاله [English] | ||
| The study of protein response of sunflower root to drought stress using proteomics approach | ||
| نویسندگان [English] | ||
| Mehdi Ghaffari1؛ Saadat Sharifi2؛ Gholamreza Bakhshi Khaniki3 | ||
| 1Seed and Plant Improvement Institute Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran | ||
| 2Former M.Sc. of Biology, Payame Noor University, Tehran, Iran | ||
| 3Professor, Department of Biology, Payame Noor University, Tehran, Iran | ||
| چکیده [English] | ||
| In order to understand of the molecular mechanisms of drought tolerance in sunflower, proteomic pattern of roots in two drought sensitive and drought-tolerant lines were evaluated under limited and favorable water conditions. After 2DE and comparison of relative abundance of protein spots using t test, 12 of 417 protein spots in sensitive and 17 of 467 in tolerant line were affected by drought stress significantly. Following nano-LC MS/MS the protein spots were identified using Mascot search engine in NCBI protein database considering more than 10 % sequence coverage and score of above 80. Cytoplasmic and nuclear proteins were the most proteins which were affected by water deficiency. Three protein spots i.e. Enolase, Glyceraldehyde 3-phosphate dehydrogenase and Chalcone synthase were expressed differentially in these lines. Reduction of Enolase as a sign of metabolic impairment could be resulted in downstream process under drought stress. Increased expression of Glyceraldehyde 3-phosphate dehydrogenase and Chalcone synthase could have a role in detoxification/removal of oxidative destruction and antioxidant capability of the tolerant line. Increased level of heat shock protein, dihydroflavonol reductase, Seed linoleate 9S-lipoxygenase, Ubiquitin carboxyl-terminal hydrolase and G protein indicated crucial role of defensive, protective and transductive process in reduction of drought injuries. | ||
| کلیدواژهها [English] | ||
| Two Dimensional Electrophoresis. Enolase, heat shock protein, Mass Spectrophotometry | ||
| مراجع | ||
|
Ashraf M, Wu L (1994) Breeding for salinity tolerance in plants. Crit. Rev. Plant Sci. 13:17-42.
Baek D, Jin Y, Jeong JC, Lee H J, Moon H, Lee J, Shin D, Kang CH, Kim DH, Nam J, Lee SY, Yun DJ (2008) Suppression of reactive oxygen species by glyceraldehyde-3-phosphate dehydrogenase. Phytochem. 69: 333-338.
Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, Bergkamp R, Dirkse W, Van Staveren M, Stiekema W, Drost L, Ridley P, Hudson S A, Patel K, Murphy G, Piffanelli P, Wedler H, Wedler E, Wambutt R, Weitzenegger T, Pohl T M, Terryn N, Gielen J, Villarroel R, De Clerck R, Van Montagu M, Lecharny A, Auborg S, Gy I, Kreis M, Lao N, Kavanagh T, Hempel S, Kotter P, Entian KD, Rieger M, Schaeffer M, Funk B, Mueller-Auer S, Silvey M, James R, MA, Pons A, Puigdomenech P, Douka A, Voukelatou E, Milioni D, Hatzopoulos P, Piravandi E, Obermaier B, Hilbert H, Dusterhoft A, Moores T, Jones JD G, Eneva T, Palme K, Benes V, Rechman S, Ansorge W, Cooke R, Berger C, Delseny M, Voet M, Volckaert G, Mewes HW, Klosterman S, Schueller C, Chalwatzis N (1998) Analysis of 1.9Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature, 391: 485-488.
Bhushan D, Pandey A, Choudhary MK, Datta A, Chakraborty S, Chakraborty N (2007) Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Mol. Cell. Proteomics, 6: 1868-1884.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
Campalans A, Pages M, Messeguer R (2001) Identification of differentially expressed genes by the cDNA-AFLP technique during dehydration of almond (Prunus amygdalus). Tree Physiol. 21: 633-643.
Caruso G, Cavaliere C, Foglia P, Gubbiotti R, Samperi R, and Laganà A (2009) Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry. Plant Sci. 177: 570-576.
Castillejo AM, Maldonado AM, Ogueta S, Jorrin JV (2008) Proteomic analysis of responses to drought stress in sunflower leaves by 2DE gel electrophoresis and mass spectrometry. Proteomics J. 1: 59-71.
Dao TTH, Linthorst HJM, Verpoorte R (2011) Chalcone synthase and its functions in plant resistance. Phytochem. Rev. 10(3): 397-412.
Dreher K, and Callis J (2006) Ubiquitin, Hormones and Biotic Stress in Plants. Section of Molecular and Cellular Biology, Plant Biology Graduate Group Program, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Ann. Bot. 99: 787-822.
Fang X, Ma H, Lu D, Yu H, Lai W and Ruan S (2011) Comparative proteomics analysis of roteins expressed in the I-1 and I-2 internodes of strawberry stolons. Proteome Sci. 9 (26), 1-15.
Fulda S, Mikkat S, Stegmann H, Horn R (2011) Physiology and proteomics of drought stress acclimation in sunflower (Helianthus annuus L.). Plant Biol. 13: 632-642.
Gao L, Yan X, Li X, Guo G, Hua Y, Ma W and Yan Y (2011) Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE). Phytochemistry, 72(10): 1180-91.
Ghaffari M, Toorchi M, Valizadeh M, Komatsu S (2013) Differential response of root proteome to drought stress in drought sensitive and tolerant sunflower inbred lines. Funct. Plant Biol. 40(6), 609-617.
Ghaffari M, Toorchi M, Valizadeh, M, Shakiba MR (2017) Proteomic prospects for tolerance of sunflower to drought stress during the flowering stage. Crop Pasture Sci. 68(5): 457-465.
Guicherd P, Peltier JP, Gout E, Bligny R, Marigo G (1997) Osmotic adjustment in Fraxinus excelsior L. malate and mannitol accumulation in leaves under drought conditions. Trees 11(3): 155-161.
Hajduch M, Casteel JE, Tang S, Hearne LB, Knapp S, Thelen JJ (2007) Proteomic analysis of near-isogenic sunflower varieties differing in seed oil traits. J. Proteome Res. 6: 3232-3241.
Hajheidari M, Eivazi A, Buchanan BB, Wong JH, Majidi I, Salekdeh GH (2007) Proteomics uncovers a role for redox in drought tolerance in wheat. J. Proteome Res. 6: 1451-60.
Joo JH. Wang S, Chen JG, Jones AM, Fedoroff NV (2005) Different signaling and cell death roles of heterotrimeric G protein alpha and beta subunits in the Arabidopsis oxidative stress response to ozone. Plant Cell 17: 957–970.
Kausar R, Arshad M, Shahzad A, Komatsu S (2013) Proteomics analysis of sensitive and tolerant barley genotypes under drought stress. Amino Acids, 44(2): 345–359.
Kottapalli KR, Rakwal R, Shibato J, Burow G, Tissue D, Burke J, Puppala N, Burow M, Payton P (2009) Physiology and proteomics of the water-deficit stress response in three contrasting peanut genotypes. Plant Cell Environ. 32: 380-407.
Kurup S, Jones HD, Holdsworth MJ (2000) Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds. Plant J. 21:143–155.
Laporte MM, Shen B, Tarczynski MC (2002) Engineering for drought avoidance: Expression of maize NADPmalic enzyme in tobacco results in altered stomatal function. J. Exp. Bot. 53: 699-705.
Li X, Sun H, Pei J, Dong Y, Wang F, Chen H, Sun Y, Wang N, Li H, Li Y (2012) De novo sequencing and comparative analysis of the blueberry transcriptome to discover putative genes related to antioxidants. Gene, 511(1):54-61.
Marian CO, Krebs SL, Arora R (2004) Dehydrin variability among Rhododendron spp: A25 kDa dehydrin is highly conserved and associated with cold acclimation across wide array of species. New Phytol. 161: 773-780.
Minarik P, Tomaskova N, Kollarova M, Antalik M (2002) Malate Dehydrogenases Structure and Function. Minireview. Gen. Phys. Biophys. 21(3), 257-265.
Mohammadi PP, Moieni A, Hiraga S, Komatsu S (2012) Organ-specific proteomic analysis of drought-stressed soybean seedlings. J. Proteomics, 75 (6): 1906–1923.
Mustafa G, Setsuko Komatsu. 2014. Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress. Frontiers in Plant Science (5) Article 627.
Ndimba BK, Chivasa S, Simon WJ, Slabas AR (2005) Identification of Arabidopsis salt and osmotic stress responsive proteins using two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics, 5: 4185–4196.
Perfus-Barbeoch L. Jones AM, Assmann SM (2004) Plant heterotrimeric G protein function: insights from Arabidopsis and rice mutants. Curr. Opin. Plant Biol. 7: 719-731.
Porta H, Rocha-Sosa M (2002) Plant lipoxygenases, physiological and molecular features. Plant Physiol. 130: 15–21.
Qian, H, Lu H, Ding H, Lavoie M, Li Y, Liu W, Fu Z (2015) Analyzing Arabidopsis thaliana root proteome provides insights into the molecular bases of enantioselective Imazethapyr toxicity. Sci. Rep. 5: 11975.
Quartacci MF and Navari-Izzo F (1992) Water stress and free radical mediated changes in sunflower seedlings. J. P. Physiol. 139: 621-625.
RiccardiF, Gazeau P, de Vienne D, Zivy M (1998) Protein changes in response to progressive water deficit in maize. Plant Physiol. 117: 1253-1263.
Schniter AA, Miller JF (1981) Description of sunflower growth stage. Crop Sci. 21: 901-903.
Singh BD (2015) Plant breeding, Principles and methods. Kalyani Publishers, New Delhi, India.
TankhaK, Gupta RK (1992) Effect of water deficit and sulphur dioxide on total soluble proteins, nitrate reductase activity and free proline content in sunflower leaves. Biol. Plantarum 34 (3-4): 305-31.
Wang W, Tai F, Chen S (2008) Optimizing protein extraction from plant tissues for enhanced proteomics analysis. J. Separation Sci. 31: 2032-2039.
Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci. 9: 244-252.
Xiaochuan S, Wang Y, Xu L, Li C, Zhang W, Luo X, Jiang H, Li L (2017) Unraveling the root proteome changes and its relationship to molecular mechanism underlying salt stress response in radish (Raphanus sativus L.). Front. Plant Sci. 8:1192.
Zhang J, Kirkham MB (1996a) Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol. 132: 361-373.
Zhang J, Kirkham MB (1996b) Enzymatic responses of the ascorbate-glutathione cycle to drought in sorghum and sunflower plants. Plant Sci. 113: 139-147. | ||
|
آمار تعداد مشاهده مقاله: 525 تعداد دریافت فایل اصل مقاله: 486 |
||