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تأثیر دی 2-اتیل هگزیل فتالات بر برخی شاخصهای موکوسی پوست کپور معمولی (Cyprinus carpio) | ||
فصلنامه علمی زیست شناسی جانوری تجربی | ||
مقاله 7، دوره 8، شماره 1 - شماره پیاپی 29، شهریور 1398، صفحه 77-88 اصل مقاله (795.36 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.30473/eab.2019.5983 | ||
نویسندگان | ||
مرتضی کمالی* 1؛ سعید علی نژادمعلم2؛ نسترن پوراحمدی3 | ||
1کارشناس ارشد رشته بیوشیمی، دانشگاه آزاد اسلامی، واحد بابل | ||
2استادیار، گروه زیستشناسی، دانشگاه آزاد اسلامی، واحد بابل . | ||
3مربی، گروه علوم و مهندسی شیلات، دانشکده منابع طبیعی و علوم | ||
چکیده | ||
چکیده دی 2-اتیلهگزیل فتالات، بهدلیل خواص مناسب و هزینه کم آن بهطور گستردهای در تولید محصولات PVC کاربرد دارد. در اثر رهاسازی این مواد با خواص شبه استروژنی و تأثیرات آن بر بقای اکوسیستمها، سلامت و جمعیت آبزیان و انسانها از اهمیت ویژهای برخوردار است. در مطالعه حاضر، اثر سمیت دو غلظت مختلف DEHP بر برخی شاخصهای موکوسی کپور معمولی (Cyprinus Carpio) بررسی شد. 108 عدد بچهماهی کپور معمولی به وزن 22/2±60/17 گرم از مراکز پرورش ماهیان گرمابی تهیه و پس از انتقال به تانکهای 100 لیتری قرار داده شدند. آزمایش شامل سه تیمار و سه تکرار طراحی شد. در انتهای دوره الگوی پروتئینی و پروفیل اسیدآمینه موکوس پوست ماهی کپور ارزیابی شد. نتایج آزمایشات جهت مقایسه الگوی پروتئینی موکوس کپورماهی معمولی در معرض DEHP با دو غلظت مختلف قرار گرفته با نمونه شاهد (بدون مواجهه با DEHP) نشان داد که تفاوت قابلتوجهی در تراکم باندها بین الگوی پروتئنی دو نمونه مشهود میباشد. اسیدهای آمینه موکوس پوست ماهی در چهار رده تقسیمبندی شدند که میانگین درصد اسیدآمینهها در تیمارهای مختلف اختلاف معنیداری در سطح 95% داشتند. با توجه به حساسیت موکوس پوست ماهی نسبت به آلایندهها و سموم میتواند نشانگر زیستی مناسبی برای نشان دادن آلایندهها و سموم باشد و مصرف بالای مواد پلاستیکی میتواند هشداردهنده برای سلامتی آبزیان و انسانها باشد. | ||
کلیدواژهها | ||
واژههای کلیدی: آلودگی؛ زیستنشانگر؛ فتالات استر؛ ماهی کپور | ||
عنوان مقاله [English] | ||
Effect of Di 2-Ethyl Hexyl phthalate on some indeces of Skin Mucus in common Carp (Cyprinus carpio) | ||
نویسندگان [English] | ||
Morteza Kamali1؛ Saeid Alinejad Moaelem2؛ Nastaran Pour-Ahmadi3 | ||
1M. A. of Biochemistry, Islamic Azad University, Babol Branch, Iran | ||
2Assistant Professor, Department of Biology, Faculty of Natural Resources and Marin Science, Tarbiat Modares University, Tehran, Iran | ||
3Instructor, Department of Fisheries Science and Engineering, Faculty of Natural Resources and Marine Sciences, Tarbiat Modarres University | ||
چکیده [English] | ||
Abstract Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and the aquatic and human populations. In present study; effects of toxicity of DEHP on protein band model and amino acid profile of mucus common carp (Cyprinus carpio) was studied. 108 common carp (17.60±2.22gr) is gathered from warm water fish center and they were allocated into 9 tanks (12 Fish per tank). Fish adapted to new condition for a week. During this time; they were feed twice a day with same diets. Fishes were exposed at 3 concentration treatments containing 0; 0.1 and 1 mg/L DEHP under laboratory condition (25±0.5°C; pH: 7.4-8) for a period of 96 hours. We assessed skin mucus protein profiles by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and we were studied amino acid profile by HPLC. The results of the experiments to compare the protein pattern of carp’s mucus (exposed to DEHP with two different concentrations) with the control sample (without exposure to DEHP) showed that there is a significant difference in the bands density between the two protein patterns. These results indicated that concentrations of DEHP beneficially affects Amino Acid profiles of skin mucus in carp and they were categorized in 4 branches. There were Significant differences between exposed and control groups. Therefore; fish skin mucus can be a biomarker for showing contaminants and toxins. And the high consumption of plastic materials can be a warning to the health of aquatic animals and humans. | ||
کلیدواژهها [English] | ||
Keywords: Biomarkers, common carp, pollution, phthalate esters | ||
مراجع | ||
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