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تغییرات شاخصهای متابولیسمی بچه ماهی سفید دریای خزر در مواجهه حاد و نیمهمزمن با ترکیبات نانوذرات اکسید مس و نانوکلوئیدنقره | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 5، دوره 8، شماره 2 - شماره پیاپی 30، آبان 1398، صفحه 51-67 اصل مقاله (515.95 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2019.6205 | ||
| نویسندگان | ||
| بهروز حیدری* 1؛ مینا احمدپور2؛ راضیه امانی3 | ||
| 1عضو هیات علمی- دانشگاه گیلان | ||
| 2کارشناس ارشد، زیستشناسی دریا، گروه زیستشناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران | ||
| 3کارشناس ارشد، زیستشناسی دریا، گروه زیستشناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران . | ||
| چکیده | ||
| چکیده افزایش کاربرد و مصرف نانوذرات در صنعت، بهداشت و کشاورزی امکان ورود این نانوذرات به محیطزیست، خصوصاً محیطهای آبی را افزایش میدهد. مطالعه حاضر، اثرات نانوذرات کلوئید نقره و اکسیدمسI (Cu2O) بر کبد و پلاسمای خون بچه ماهی سفید دریای خزر (Rutilus kutum) با سنجش میزان فعالیت آنزیمهای ALT، AST، ALP و LDH را بررسی کرد. بچه ماهیها (6/3±1/17 گرم) از مرکز تکثیر و بازسازی دکتر یوسف پور تهیه شدند. پس از تیمار بندی و در معرض قرار دادن ماهیها با نانوذرات کلوئید نقره و نانوذرات اکسیدمسI (Cu2ONPs) در غلظتهای مختلف، خونگیری در دو نوبت در روز هفتم و بیست و یکم آزمایش انجام گرفت. همزمان بافت کبد ماهیها نیز جدا شد و در فریزر 70- تا زمان انجام آنالیز پارامترها نگهداری شدند. نتایج، تغییر در فعالیت آنزیمها در کبد و پلاسمای خون بچه ماهی سفید دریای خزر در معرض با نانوذرات را نشان داد. بیشترین تغییرات در پلاسما در روز هفتم آزمایش مشاهده شد. آنزیم LDH نسبت به دیگر آنزیمها بیشترین تغییرات را در پلاسما داشت. در روز بیست و یکم آزمایش فقط آنزیم LDH در پلاسما دارای تغییرات معنیدار نسبت به شاهد بود. در کبد، سطوح آنزیم های ALT، AST، ALP و LDH در معرض نانوذرات تقریبا افزایش یافتند. در کبد همانند پلاسما بیشترین تغییرات متعلق به آنزیم LDH بود. درمجموع، تغییر در آنزیمهای متابولیسمی نشاندهنده تغییر در وضعیت فیزیولوژیک ماهی تحت استرس ایجادشده توسط نانوذرات که میتواند سلامت آبزی را به خطر بیاندازد. | ||
| کلیدواژهها | ||
| واژههای کلیدی: نانوذرات؛ آنزیم؛ آلایندهها؛ استرس؛ ماهی | ||
| عنوان مقاله [English] | ||
| Changes in metabolic indexes of the Caspian kutum fry in acute and subacute exposure to the nanoparticle of copper oxide and silver nanocolloid | ||
| نویسندگان [English] | ||
| behrrouz heidari1؛ Mina Ahmadpour2؛ Razyeh Amani3 | ||
| 2M.A. in Marine Biology, Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran | ||
| 3M.A. in Marine Biology, Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated on the liver and blood plasma of the Caspian kutum (Rutilus kutum) fry by measuring the activity of the ALT, AST, ALP and LDH enzymes. The samples (17.1±3.6 g) were prepared from the Propagation and Reconstruction Center of Dr. Yousoufpour. After exposing the fish to different concentrations of the nano colloidal silver and Cu2ONPs, the blood was collected on the 7th and 21st days of the experiment. At the same time, the liver tissue of the fish was removed and stored in the -70oC until analyzes of the parameters. The results showed changes in the activity of the enzymes in the liver and plasma of the Caspian kutum fry. The most changes of enzymes were observed in the plasma on the 7th day of the experiment. The LDH enzyme had the most changes in the plasma other than the enzymes. On the 21st day, only the plasma LDH had a significant variation. In the liver, the levels of ALT, AST, ALP and LDH enzymes were almost increased during exposure to the nanoparticles. In the liver, as with plasma, the most changes of the enzymes belonged to the LDH. In conclusion, fluctuations in the metabolic enzymes indicate a change in the physiological state of fish under nanoparticles stress that can threaten aquatic health. | ||
| کلیدواژهها [English] | ||
| Keywords: Enzyme, fish, nanoparticles, pollutants, stress | ||
| مراجع | ||
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