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Spectrophotometric Determination of Nalidixic Acid in Some Real Samples After Preconcentration with GO/ZnO Nanocomposite-Based Pipette Tip Micro-Solid Phase Microextraction | ||
Iranian Journal of Analytical Chemistry | ||
مقاله 3، دوره 7، شماره 1 - شماره پیاپی 13، خرداد 2020، صفحه 21-29 اصل مقاله (794.69 K) | ||
نوع مقاله: Full research article | ||
شناسه دیجیتال (DOI): 10.30473/ijac.2019.49189.1157 | ||
نویسندگان | ||
Sayyed Hossein Hashemi* 1؛ Fateme Keykha2 | ||
1Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, P.O. Box 98617-85553, Chabahar, Iran. | ||
2Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, P.O. Box 98617-85553, Chabahar, Iran | ||
چکیده | ||
In this research a graphene oxide/zinc oxide nanocomposite (GO/ZnO) was synthesized and employed for simple and sensitive pipette tip-based micro-solid phase extraction (PT-µSPE) of nalidixic acid (NA) from seawater and human blood plasma samples following by its determination by spectrophotometry. Several parameters affecting the suggested protocol were optimized, namely type and volume of eluent, amount of sorbent, sample volume, number of cycles of extraction and elution, pH of sample solution and type and amount of salt. The optimization performed by both response surface methodology (RSM) and one-variable-at-a-time techniques. Figures of merit were achieved as: detection limit; 0.30 µg L-1, enrichment factor 40 for NA, linear calibration curve in the range of 1.0-200.0 µg L-1; and reproducibility (as RSD %) better than 4.4%. Results for the application of the technique in seawater and human blood plasma showed that this analysis method can be applied for the determination of the analyte in complex real samples successfully. | ||
کلیدواژهها | ||
Nalidixic Acid؛ Seawater؛ Graphene Oxide/Zinc Oxide Nanocomposite؛ Pipette Tip Solid Phase Extraction؛ Response Surface Methodology | ||
عنوان مقاله [English] | ||
تعیین اسپکتروفتومتری نالیدیکسیک اسید در برخی نمونههای حقیقی بعد از پیشتغلیظ با استخراج فاز جامد در سرسمپلر به کمک جاذب نانوکامپوزیت گرافن اکسید/ روی اکسید | ||
نویسندگان [English] | ||
سید حسین هاشمی1؛ فاطمه کیخا2 | ||
1گروه شیمی دریا، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی، چابهار، ایران | ||
2گروه شیمی دریا، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی، چابهار، ایران | ||
چکیده [English] | ||
در این کار تحقیقاتی یک نانو کامپوزیت گرافن اکسید/ روی اکسید (GO/ZnO) سنتز گردید و برای استخراج فاز جامد در سر سمپلر نالیدیکسیک اسید از نمونههای آب دریا و پلاسمای خون انسان و تعیین آن با اسپکتروفتومتری بکار رفت. پارامترهای مؤثر بر روش پیشنهادی، از جمله نوع و حجم حلال شوینده، مقدار جاذب، حجم نمونه، تعداد سیکلهای استخراج و شویش، pH محلول نمونه، نوع و مقدار نمک بررسی و بهینه سازی شدند. بهینهسازی با استفاده از روشهای یک متغیر در یک زمان و سطح پاسخ انجام گردید. ارقام شایستگی روش به صورت زیر بدست آمد: حدّ تشخیص روش 30/0 میکروگرم بر لیتر، فاکتور پیشتغلیظ 40 برای NA، محدودة خطّی منحنی درجه بندی 0/200- 0/1 میکروگرم بر لیتر و با تکرارپذیری (RSD) بهتر از 4/4% حاصل گردید. نتایج کاربرد روش در آب دریا و پلاسما خون انسان نشان داد که این روش تجزیهای میتواند برای تعیین آنالیت در نمونههای واقعی پیچیده به طور موفقیت آمیزی بکار رود. | ||
کلیدواژهها [English] | ||
نالیدیکسیک اسید, آب دریا, گرافن اکسید/ روی اکسید, استخراج فاز جامد در سرسمپلر, روششناسی سطح پاسخ | ||
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