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مطالعه بیوانفورماتیکی خانوادة ژنی آنتی‌پورترهای کلسیم/کاتیون (CaCAs) در ذرت (Zea mays L.) | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 2، دوره 9، شماره 29 - شماره پیاپی 3، خرداد 1399، صفحه 21-37 اصل مقاله (1.13 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2020.52373.1803 | ||
| نویسندگان | ||
| بهناز کرمی لاکه1؛ محمد مهدی سوهانی2؛ امین عابدی* 3 | ||
| 1دانش‌آموخته کارشناسی‌ارشد بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران. | ||
| 2دانشیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران. | ||
| 3دانش‌آموخته دکترای بیوتکنولوژی کشاورزی-گیاهی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران. | ||
| چکیده | ||
| پروتئین‌های خانواده آنتی‌پورتر یون کلسیم/کاتیون (CaCA) نقش حیاتی در هموستازی یون کلسیم دارند که یک رویداد مهم در طول نمو و پاسخ دفاعی گیاه است. در مطالعه حاضر با استفاده از داده‌های بانک‌های اطلاعاتی مرتبط، 14 ژن CaCAs در ژنوم ذرت شناسایی و براساس سازماندهی ساختاری و ارتباط تکاملی آن‌ها با پروتئین‌های شناسایی‌شده به سه گروه CAX، CCX و MHX طبقه‌بندی شدند. بیشتر پروتئین‌های ZmCaCA دارای دو دمین Na_Ca_ex بودند. تمامی ژن‌های شناسایی‌شده دارای حداقل یک موتیف کارکردی بوده و ساختار ژنی هر زیر گروه CaCA بسیار حفاظت شده است. در پیش‌بینی مولکول-های miRNA واکنش‌گر نسبت به ژن‌های CaCA در ذرت 33 نوع miRNA متفاوت شناسایی شد که در تنظیم بیان پس از رونویسی 13 ژن CaCAs از طریق برش mRNA یا ممانعت از ترجمه دخالت دارند. علاوه بر این، چندین عنصر تنظیمی cis پاسخ به تنش‌ها و هورمون‌ها در پیش‌بر این ژن‌ها شناسایی شد. بیان متغیر اکثر ژن‌های ZmCaCA در مراحل مختلف رشد و نمو، نقش مشخص آن‌ها در نمو ذرت را نشان می-دهد. همچنین القاء بیان این ژن‌ها در پاسخ به تنش‌های غیر زیستی (سرما، شوری، خشکی) کارکرد دفاعی ژن‌های ZmCaCA را مشخص نمود. بیشترین افزایش و کاهش بیان ژن مربوط به ژن‌های CAX است. نتایج این مطالعه اطلاعات پایه در مورد روابط فیلوژنی و کارکردهای احتمالی ژن‌های CaCA ذرت را برای پژوهش‌های آتی فراهم می‌سازد. | ||
| کلیدواژه‌ها | ||
| بررسی فیلوژنتیکی؛ بیوانفورماتیک؛ پیش بر؛ ریزآرایه | ||
| موضوعات | ||
| بیوانفورماتیک | ||
| عنوان مقاله [English] | ||
| Bioinformatical study of Calcium/cation (CaCA) antiporters gene family in maize (Zea mays L.) | ||
| نویسندگان [English] | ||
| Behnaz Karami Lake1؛ Mohammad Sohani2؛ Amin Abedi3 | ||
| 1M.Sc. Graduated, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Associate Professor, Department of Agricultural Biotechnology, Faculty of Agriculture Science, University of Guilan, Rasht. Iran | ||
| 3PhD Graduated, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| The Ca2+/cation antiporters (CaCA) superfamily proteins play vital function in Ca2+ ion homeostasis, which is an important event during development and defense response. In the present study, using related database, 14 CaCA genes were identified in the maize genome and classified according to their structural organization and evolutionary association with the identified CAX, CCX and MHX proteins. Most of the ZmCaCA proteins had two Na_Ca_ex domains. All of the identified genes had at least one functional motif and gene structure of each CaCA subgroup is highly conserved. In the prediction of reactive miRNAs relative to CaCA genes in maize, 33 different miRNA variants were identified that regulate the expression of 13 CaCA genes through cleavage or inhibition of translation. In addition, several cis-acting regulatory elements in ZmCaCA genes were found to be related to hormones stress responses. The variable expression of most ZmCaCA genes at different stages of development indicates their distinct role in development. Expression of these genes in abiotic stresses (cold, salt, and drought) indicates their role in stress response. The greatest high expression and down regulation of gene expression is related to CAX genes. The results of this study provide basic data about phylogeny and putative function of these genes for future studies on the role of CaCA genes in maize. | ||
| کلیدواژه‌ها [English] | ||
| Bioinformatics, Microarray, Phylogenetic analysis, Promoter | ||
| مراجع | ||
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