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بررسی تغییرات برخی از پارامترهای خون‌شناسی و ایمنی ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) در آبهای شیرین، شور و لبشور | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| مقاله 8، دوره 9، شماره 1، شهریور 1399، صفحه 89-101 اصل مقاله (1.1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2020.43864.1672 | ||
| نویسندگان | ||
| سمیرا جعفریان1؛ محمدرضا بیواره* 2؛ حجت الله جعفریان3 | ||
| 1دکتری، گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبدکاووس، گنبدکاووس، ایران | ||
| 2کارشناس، گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبدکاووس، ایران | ||
| 3دانشیار، گروه شیلات، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبدکاووس، گنبدکاووس، ایران | ||
| چکیده | ||
| هرگونه تغییر در پارامترهای خونی و بیوشیمیایی سرم می‌تواند ناشی از تغییرات نامطلوب شرایط و یا فاکتورهای استرس‌زا در محیط‌زیست ماهی باشد. در مطالعه حاضر تأثیر غلظت‌های مختلف شوری بر پارامترهای خون‌شناسی و ایمنی ماهی قزل‌آلای رنگین‌کمان در آبهای با شوری 5/0 قسمت در هزار (آب شیرین)، 02/3 قسمت در هزار (آب لبشور) و 20/18 قسمت در هزار (آب شور) در سه ایستگاه مختلف در استان گلستان بررسی شد. بچه‌ماهیان با میانگین وزنی 80 گرم به‌مدت 5 ماه با استفاده از جیره‌های تجاری مخصوص ماهی قزل‌آلا تغذیه شدند. در پایان دوره، نمونه‌برداری از خون 60 عدد ماهی به‌ظاهر سالم با میانگین وزنی 250 گرم از هر ایستگاه انجام شد. نمونه‌های سرم با کمک دستگاه سانتریفیوژ از سلول‌های خون جداسازی شد. پارامترهای مورد نظر با استفاده از روش‌های متداول آزمایشگاهی و اندیس‌های گلبولی با استفاده از فرمول‌های مربوطه اندازه‌گیری شد. نتایج به‌دست‌آمده اختلاف معنی‌داری در تعداد گلبولهای سفید خون و ایمنوگلوبولین M بین هر سه گروه آزمایشی نشان داد (05/0p<). بیشترین تعداد گلبولهای سفید (310±14300 عدد در میلیمتر مکعب) در خون ماهیان پرورشی با آب لبشور و بالاترین میزان ایمنوگلوبولین M (007/0±428/0 گرم در لیتر) در خون ماهیان پرورشی با آب شور ثبت شد (05/0p<). تعداد گلبولهای قرمز، درصد هماتوکریت، غلظت هموگلوبین، حجم متوسط گلبولی، متوسط هموگلوبین گلبولی و مسیر فرعی کمپلمان (C3 و C4) نیز در خون ماهیان پرورشی با آب لبشور در مقایسه با همنوعان پرورشی آنها در آبهای شیرین و شور از کاهشی معنیدار برخوردار بود (05/0p<)؛ اما اختلاف معنی‌داری در سطح این شاخص‌ها بین ماهیان پرورشی با آبهای شیرین و شور مشاهده نشد (05/0p>). غلظت متوسط هموگلوبین گلبولی نیز بین تیمارهای مختلف آزمایشی فاقد اختلاف معنی‌داری بود (05/0p>). درمجموع نتایج مطالعه حاضر نشان داد که پرورش ماهی قزل‌آلا به‌عنوان یک گونه یوریهالین در آبهای با شوری 18~ قسمت در هزار به‌دلیل تمایل به بازگشت این شاخص‌ها به سطوح اندازه‌گیری‌شده آنها در آب شیرین از وضعیت مطلوب‌تری برخوردار بوده و این گونه در شوری مذکور کمتر تحت تأثیر عامل استرس قرار میگیرد. | ||
| کلیدواژه‌ها | ||
| ایمنی؛ پارامترهای خون‌شناسی؛ شوری؛ قزل‌آلای رنگین‌کمان | ||
| عنوان مقاله [English] | ||
| Comparative survey of some hematological and immunity parameters in rainbow trout (Oncorhynchus mykiss) cultured in fresh, saline and brackish waters | ||
| نویسندگان [English] | ||
| Samira Jafaryan1؛ Mohammadreza Bivareh2؛ Hojatollah Jafaryan3 | ||
| 1Ph.D., Department of Fisheries, Faculty of Agriculture and natural resource, Gonbad Kavous University, Gonbad Kavous, Iran | ||
| 2M.A., Department of Fisheries Group, Faculty of Agriculture and natural resource, Gonbad Kavous University, Gonbad Kavous, Iran | ||
| 3Associate Professor, Department of Fisheries, Faculty of Agriculture and natural resource, Gonbad Kavous University, Gonbad Kavous, Iran | ||
| چکیده [English] | ||
| Any change in hematological and biochemical parameters of plasma could be a predictor of unfavorable environment or effect of different stress factors in fish. The present study was designed to assess different salinity concentration induced changes hematological parameters and immunity indices in rainbow trout fishes reared in fresh (0.5ppt), brackish (3.02ppt) and salt water (18.20ppt) at three different station in Golestan Province. The experiment was beginning with the rainbow trout juvenile with initial average 50g then run for 5 months using the commercial trout feed. At the end of rearing periods, blood samples were taken from 20 individuals was apparentlyhealthy with the mean weight of 250g from each station and serum was separated. Serum was separated with centrifuge machine and blood parameters were analyzed by the routine method used in fish hematology. The results showed a significant difference between WBC and IgM value in tree experimental group (p<0.05). The highest WBC (14300±310 103/mm) was found in the blood of fish fry reared in brackish water and the highest IgM value (0.428±0.007 g/l) was recorded in fish fry reared in saline water (p<0.05). RBC, Hb, Ht, MCV, MCH, C3 and C4 significantly decreased in the blood of fish reared in brackish water (p<0.05). But, these indices not showed any significant difference among the fish reared in the fresh and saline water (p>0.05). But there was no significant difference in the level of these indices between cultivated fish fry with fresh and brackish waters (p> 0.05). Also, was observed no significant differences among the MCHC value in experimental group (p>0.05). In conclusion, the results of this study indicated that rearing of O. mykiss as a euryhaline species in saline water of ~18 ppt is more desirable due to the tendency to return these indices to measured level in fresh water and are less affected by stress. | ||
| کلیدواژه‌ها [English] | ||
| Hematological parameters, Immunity, Rainbow trout, Salinities | ||
| مراجع | ||
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