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ارزیابی خصوصیات آنتیاکسیدانی و ضدمیکروبی پروتئین هیدرولیزشده ماهی حسون ( Saurida tumbil ) | ||
| فصلنامه علمی زیست شناسی جانوری تجربی | ||
| دوره 9، شماره 2 - شماره پیاپی 34، آبان 1399، صفحه 23-35 اصل مقاله (1.26 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/eab.2020.45855.1706 | ||
| نویسندگان | ||
| سمیه بهرام* 1؛ محمد خضری2؛ سید روح الله جوادیان1 | ||
| 1گروه شیلات، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران | ||
| 2گروه شیلات، دانشکده منابع طبیعی، دانشگاه کردستان، کردستان، سنندج | ||
| چکیده | ||
| امروزه با آشکار شدن سمیت و عوارض جانبی نگهدارنده‌های سنتزی مصرفکنندگان به‌دنبال ترکیبات طبیعی با اثرات آنتیاکسیدانی و ضدمیکروبی هستند. در پژوهش حاضر عضله ماهی حسون به‌کمک آنزیم آلکالاز در دو غلظت 2 و 4 درصد و در زمانهای 90 و 180 دقیقه هیدرولیز شد. فعالیت آنتیاکسیدانی پروتئین‌های هیدرولیزه توسط فعالیت حذف‌کنندگی رادیکال‌های آزاد DPPH، ABTS و هیدروکسیل، فعالیت شلاته‌کنندگی فروس و کاهندگی فریک بررسی شد. فعالیت ضدمکیروبی با روش انتشار دیسک و MIC تعیین شد. پروتئین‌های هیدرولیز شده توسط آنزیم آلکالاز فعالیت قابل‌ملاحظه‌ای در حذف رادیکال‌های DPPH (92/37درصد)، ABTS (34/77 درصد)، و هیدروکسیل (79/21درصد) و شلاته کنندگی یون فروس (95/26 درصد) و فعالیت ضعیفی در کاهندگی یون فریک (جذب نوری 00/0±/13/0 در طول موج 700) نشان دادند. در مطالعه حاضر بیشترین فعالیت مهار رادیکال‌های DPPH (8/2ic50=) و هیدروکسیل (59/8IC50=) توسط نمونه هیدرولیزشده با 4 درصد آنزیم به‌مدت 180 دقیقه مشاهده شد. بیشترین بازیافت پروتیئنی نیز توسط نمونه فوق مشاهده شد. نمونه‌های پروتیئن هیدرولیز ماهی حسون فاقد فعالیت ضدباکتریایی بر علیه 5 سویه باکتری بودند. پروتیئن هیدرولیزشده ماهی حسون دارای فعالیت آنتیاکسیدانی بالقوه بوده و می‌تواند به عنوان آنتی‌اکسیدان ارزان قیمت جهت استفاده در صنایع غذایی، غذاهای فراسودمند و خوراک دام توصیه شود. | ||
| کلیدواژه‌ها | ||
| آلکالاز؛ رادیکال‌های آزاد؛ ماهی حسون؛ ویژگی زیست‌فعال | ||
| عنوان مقاله [English] | ||
| Evaluation of antioxidant and antimicrobial properties of hydrolyzed protein of Saurida tumbil | ||
| نویسندگان [English] | ||
| Somayeh Bahram1؛ Mohammad Khezri2؛ Seyed Rohollah Javadian1 | ||
| 1Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran | ||
| 2Department of Fisheries, Natural Resources Faculty, University of Kurdistan, Sanandaj, Kurdistan, Iran | ||
| چکیده [English] | ||
| Todays, with the clarifying of the toxicity and side effects of synthetic preservatives, consumers are looking for natural compounds with anti-oxidant and antimicrobial effects. In the present study, lizardfish (Saurida tumbil) muscle was hydrolyzed by Alcalase at two concentrations of 2 and 4% and two times of 90 and 180 min. Then the antioxidant activity of fish protein hydrolysates (FPH) was evaluated by measuring DPPH, ABTS and OH free radical scavenging activity, and reducing power and ferrous ion-chelating assays. Antimicrobial activity was determined by disc diffusion and mic test. Alcalase enzymes hydrolyzed proteins showed remarkable activity in removing DPPH (37.92%), ABTS (77.34%) and hydroxyl (21.79%), as well as ferrous ion (26.95%) and poor activity in ion ferric degradation (Optical absorption of 0.001 ± 0.00 at a wavelength of 700). In the present study, the most inhibitory activity of DPPH and OH radicals was observed by hydrolyzed samples with 4% enzyme for 180 minutes. The most protein recovery yield was observed by the above sample. FPH showed no antibacterial activity against the studied bacterial strains. FPH of lizard fish with potential antioxidant activity can be recommended as an inexpensive antioxidant for use in the food industry, functional foods and animal feed. | ||
| کلیدواژه‌ها [English] | ||
| Alcalase, Bioactive properties, Free radical, Lizardfish | ||
| مراجع | ||
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