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مطالعه تأثیر القاگرهای اسید سالیسیلیک و اسید آزلائیک بر برخی خصوصیات بیوشیمیایی و مولکولی در پاسخ به بیماری سفیدک داخلی در Cucumis sativus L. | ||
| فصلنامه علمی زیست فناوری گیاهان زراعی | ||
| مقاله 1، دوره 10، شماره 2 - شماره پیاپی 32، اسفند 1399، صفحه 1-15 اصل مقاله (1.03 M) | ||
| نوع مقاله: علمی پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30473/cb.2021.55580.1831 | ||
| نویسندگان | ||
| هانیه عابدین پور* 1؛ ناد علی بابائیان جلودار2؛ علی دهستانی کلاگر3؛ قربانعلی نعمت زاده2 | ||
| 1گروه ژنتیک و به‌نژادی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| 2استاد، گروه ژنتیک و به‌نژادی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| 3استادیار، پژوهشکده ژنتیک و زیست‌فناوری طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| چکیده | ||
| سفیدک داخلی (Pseudoperonospora cubensis (P. cubensis یکی از مهمترین بیماریهای گیاه خیار است که موجب کاهش تولید آن در جهان می‌شود. با وجود در دسترس بودن بعضی از قارچ‌کش‌ها برای کاهش خسارت این بیماری، تمایل به استفاده از محصولات طبیعی جدید و قابل اطمینان افزایش یافته است. در این پژوهش اثر دو ماده اسید سالیسیلیک (SA) و اسید آزلائیک (AZA) با غلظت ی‍ک میلی‌مولار بر برخی خصوصیات بیوشیمیایی و بیان برخی ژن‌های دفاعی در گیاه خیار به‌صورت آزمایش اسپلیت - فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار مورد مطالعه قرار گرفت. بررسی بیان ژن نشان داد با وجود این‌که بعد از مایه‌زنی گیاهان با قارچ، بیان هر دو ژن کیتیناز بتا-1 و 3- گلوکاناز به‌طور معنی‌داری افزایش یافت، مقدار بیان ژن کیتیناز در گیاهان تیمارشده با SA و سطح بیان ژن بتا-1 و 3- گلوکاناز در گیاهان تیمارشده با AZA بیشتر بود. همچنین نتایج این بررسی نشان داد که مقدار کلروفیل a، b و فلاونوئید در گیاهان تیمارشده با AZA و SA در 6 ساعت بعد از مایه‌زنی با قارچ در مقایسه با شاهد بیشتر بود. مقدار فنل کل هم در گیاهان تیمارشده با SA و AZA نسبت به شاهد بیمار به‌ترتیب درساعات 6 و 72 بعد از تیمار افزایش یافته بود. این نتایج نشان می‌دهد که پیش تیمار گیاهان خیار با القاگرهای SA و AZA موجب پاسخ‌های سریع‌تر و افزایش کارایی سیستم دفاعی در برابر بیماری سفیدک داخلی می‌شود. | ||
| کلیدواژه‌ها | ||
| بیماری سفیدک داخلی؛ اسید سالسیلیک؛ اسید آزلائیک؛ خیار؛ القاگر | ||
| موضوعات | ||
| بیوتکنولوژی بیماریهای گیاهی | ||
| عنوان مقاله [English] | ||
| Effect of exogenous Salicylic Acid and Azelaic Acid on some biochemical and molecular responses to downy mildew disease in Cucumis sativus L. | ||
| نویسندگان [English] | ||
| Hanieh Abedinpour1؛ NadAli Babaean Jeloudar2؛ Ali Dehestani3؛ GhorbanAli Nematzadeh2 | ||
| 1Plant breeding and Biotechnology department, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran | ||
| 2Professor, Sari Agriculture Science and Natural Resource University (SANRU), Sari, Iran | ||
| 3Assistant Professor, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran | ||
| چکیده [English] | ||
| Downy mildew is one of the most important diseases of cucumber plants reducing production in the world. Despite the availability of some fungicides to control the disease, demands for new and reliable natural products has been increased. In this study, the effects of salicylic acid (SA) and Azelaic acid (AZA) on some biochemical properties and expression of defense genes in cucumber plants were studied. Gene expression analysis indicated that expression of both β-1,3- glucanase and chitinase genes were increased after inoculation with Pseudoperonospora cubensis, but this increase was much higher for chitinase gene in SA-treated plants and for β-1,3- glucanase in AZA-treated plants. Also, chlorophyll a, b and total flavonoids contents in AZA and SA-treated plants were higher compared to control 6 hours after inoculation with pathogen. Total phenolic content was also increased in SA and AZA treated plants compared to the control 6 and 72 hours after treatment, respectively. These findings indicate that pretreatment of cucumber plants with SA and AZA prompts faster defense responses and increases the efficiency of the defense system against downy mildew. | ||
| کلیدواژه‌ها [English] | ||
| Downy mildew, Salicylic acid, Azelaic acid, Cucumber, resistance inducer | ||
| مراجع | ||
|
Ali S, Ganai BA, Kamili AN, Bhat AA, Mir ZA, Bhat JA, Tyagi A, Islam ST, Mushtaq M, Yadav P (2018) Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance. Microb. Res. 212: 29-37.
Amin A, Rashad M, El-Abagy H (2007) Physiological effect of indole-3-butyric acid and salicylic acid on growth, yield and chemical constituents of onion plants. J. Appl. Sci. Res. 3(11): 1554-1563.
Anderson AJ, Blee KA, Yang KY (2006). Commercialization of plant systemic defense activation: Theory, problems and successes. Multigenic and Induced Systemic Resistance in Plants, Springer US: 386-414.
Butt UR, Naz R, Nosheen A, Yasmin H, Keyani R, Hussain I, Hassan MN (2019) Changes in pathogenesis-related gene expression in response to bioformulations in the apoplast of maize leaves against Fusarium oxysporum. J. Plant Inter. 14: 61-72.
Castro M, Fontes W (2005) Plant Defense and Antimicrobial Peptides. Protein & Peptide Letters. 12: 11-16.
Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen U, Vad K (1993) Plant chitinases. Plant. J. 3: 31-40.
Coqueiro DSO, De Souza AA, Takita MA, Rodrigues CM, Kishi LT, Machado MA (2015) Transcriptional profile of sweet orange in response to chitosan and salicylic acid. BMC Genomics. 16: 288.
Djami-Tchatchou AT, Ncube EN, Steenkamp PA, Dubery IA (2017) Similar, but different: Structurally related azelaic acid and hexanoic acid trigger differential metabolomic and transcriptomic responses in tobacco cells. BMC Plant. Bio. 17: 227.
El-Sharkawy EES, Abdalla MY, El-Shemy AO (2016) Effect of some resistance chemical inducers on incidence of cucumber Fusarium wilt caused by Fusarium oxysporum f. Sp. cucumerinum. Egyptian J. Bio. Pest Control. 26: 1-6.
FAO. (2019). "Production quantities of Cucumbers and gherkins by country." from http://www.fao.org/faostat/en/#data/QC/visualize.
Farajollahi F, Babaezadeh V, Rahimian H (2018) Induction of several Pathogenesis-Related genes in tobacco plants treated by salicylic and jasmonic acid after challenging with Pseudomonas syringae pv. Tabaci. Agri. Biotech. J. 9(4): 129-150.
Farouk S, Ghoneem KM, Ali AA (2008) Induction and Expression of Systemic Resistance to Downy Mildew Disease in Cucumber by Elicitors. Egypt J. Phyto. 36: 95-111.
Gerami E, Azimi H, Beigi F (2019) Evaluation of the Efficacy of ametoctradin+dimethomorph (Orvego® SC 525) and mandipropamid+ difenoconazole (Carial Star®SC500) Fungicides against Cucumber Downy Mildew Disease, Caused by Pseudoperonospora cubensis. Pest. Plant Protec Sci. 8(1): 26-37.
Gogoi R, Singh DV, Srivastava KD (2001) Phenols as a biochemical basis of resistance in wheat against Karnal bunt. Plant Pathol. 50: 470-476.
Haggag WM (2002) Application of epidermal coating antitranspirants for controlling cucumber downy mildew in greenhouse. Plant Pathol. 11: 69-78.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, Van Der Vossen EaG, Wu Y, Guo J, He J, Jia Z, Ren Y, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim J-Y, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Liu S, Li J, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Li G, Fang L, Li Y, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Zheng H, Li S, Zhang X, Yang H, Wang J, Sun R, Zhang B, Jiang S, Wang J, Du Y, Li S (2009) The genome of the cucumber, Cucumis sativus L. Nat. Genet. 41: 1275-1281.
Jendoubi W, Harbaoui K, Hamada W (2015) Salicylic acid-induced resistance against Fusarium oxysporum f.s.pradicis lycopercisi in hydroponic grown tomato plants. J. New Sci. 21: 985-995.
Jung HW, Tschaplinski TJ, Wang L, Glazebrook J, Greenberg JT (2009) Priming in systemic plant immunity. Sci. 324: 89-91.
Khan MIR, Fatma M, Per TS, Anjum NA, Khan NA (2015) Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants. Front. Plant Sci. 6: 462.
Kuć J (2001). Concepts and direction of induced systemic resistance in plants and its application. Eur. J. Plant Pathol. 107: 7-12.
Kumaraswamy R, Kumari S, Choudhary RC, Sharma S, Pal A, Raliya R, Biswas P, Saharan V (2019) Salicylic acid functionalized chitosan nanoparticle: a sustainable biostimulant for plant. Int. J. Biol. Macromol. 123: 59-69.
Leslie CA, Romani RJ (1986) Salicylic acid: a new inhibitor of ethylene biosynthesis. Plant Cell Rep. 5(2): 144-146.
Liang C, Hao J, Meng Y, Luo L, Li J (2018) Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress. PloS one. 13(3): e0194436.
Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans 11: 591-592.
Lim S, Borza T, Peters RD, Coffin RH, Al-Mughrabi KI, Pinto DM, Wang-Pruski G (2013) Proteomics analysis suggests broad functional changes in potato leaves triggered by phosphites and a complex indirect mode of action against Phytophthora infestans. J. Proteom. 93: 207-223.
Liu D, Xin M, Zhou X, Wang C, Zhang Y, Qin Z (2017) Expression and functional analysis of the transcription factor-encoding Gene CsERF004 in cucumber during Pseudoperonospora cubensis and Corynespora cassiicola infection. BMC Plant Biol. 17: 96.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods. 25(4): 402-408.
Małolepsza U, Rózalska S (2005) Nitric oxide and hydrogen peroxide in tomato resistance: Nitric oxide modulates hydrogen peroxide level in o-hydroxyethylorutin-induced resistance to Botrytis cinerea in tomato. Plant Physiol Biochem. 43: 623-635.
Marinova D, Ribarova F, Atanassova M (2005) Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J. Univ. Chem. Technol. Metallurgy. 40(3): 255-260.
Matern U, Kneusel RE (1988). Phenolic compounds in plant disease resistance. Phytoparasitica: 153-170.
Mauch-Mani B (1998) Salicylic Acid and Systemic Acquired Resistance to Pathogen Attack. Ann. Bot. 82: 535-540.
Metraux JP, Burkhart W, Moyer M, Dincher S, Middlesteadt W, Williams S, Payne G, Carnes M, Ryals J (1989) Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional lysozyme/chitinase. PNAS. 86: 896-900.
Moradi N, Rahimian H, Dehestani A, Babaeizad V (2016) Cucumber Response to Sphaerotheca fuliginea: Differences in Antioxidant Enzymes Activity and Pathogenesis-Related Gene Expression in Susceptible and Resistant Genotypes. J. Plant Mol. Breeding. 4: 33-40.
Murphy AM, Holcombe LJ, Carr JP (2000) Characteristics of salicylic acid-induced delay in disease caused by a necrotrophic fungal pathogen in tobacco. Physiol. Mol. Plant Pathol. 57: 47-54.
Olczak-Woltman H, Marcinkowska J, Niemirowicz-Szczytt K (2011) The genetic basis of resistance to downy mildew in Cucumis spp.—latest developments and prospects. J. Appl. Genet. 52(3): 249-255.
Possingham JV (1980) Plastid replication and development in the life cycle of higher plants. Annu. Rev. Plant Physiol. 31(1): 113-129.
Ramezani M, Ramezani F, Rahmani F, Dehestani A (2018) Exogenous potassium phosphite application improved PR-protein expression and associated physio-biochemical events in cucumber challenged by Pseudoperonospora cubensis. Sci. Horti. 234: 335-343.
Sabbagh S, Zinati FF (2016) The effect of salicylic acid to induce systemic resistance in cucumber plant to damping-off disease caused by Pythium aphanidermatum. J Applied Res. Plant Protec. 5(2):27-43.
Salzman RA, Tikhonova I, Bordelon BP, Hasegawa PM, Bressan RA (1998) Coordinate accumulation of antifungal proteins and hexoses constitutes a developmentally controlled defense response during fruit ripening in grape. Plant Physiol. 117: 465-472.
Shah J, Zeier J (2013). Long-distance communication and signal amplification in systemic acquired resistance. Front. Plant Sci. 4: 30.
Shehata S, Ibrahim S, Zaghlool S (2001) Physiological response of flag leaf and ears of maize plant induced by foliar application of kinetin, KIN and acetyl salicylic acid, ASA. Annals Agri. Sci, Ain Shams Univ.(Egypt).
Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 299: 152-178.
Sujoy S, Ashtekar N, Rai A, Sharma B, Balaraman A (2017) Synergistic effect of Benalaxyl 8% and Mancozeb 65% WP in combating downy mildew of cucumber. J. Mycopathol. Res. 55(3): 227-230.
Van Loon LC, Van Strien EA (1999) The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol. Mol. Plant Pathol. 55: 85-97.
Vermerris W, Nicholson R (2006) Phenolic compound biochemistry. Springer Science & Business Media. 1-276.
Walters DR, Ratsep J, Havis ND (2013). Controlling crop diseases using induced resistance: Challenges for the future. J. Exp. Bot. 64: 1263-1280.
Xu BJ, Chang SKC (2007) A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci. 72(2):159-166.
Xu W-Z, Deng X-P, Xu B-C, Gao Z-J, Ding W-L (2014) Photosynthetic activity and efficiency of Bothriochloa ischaemum and Lespedeza davurica in mixtures across growth periods under water stress. Acta Physiol. Plant. 36(4): 1033-1044.
Yakhin OI, Lubyanov AA, Yakhin IA, Brown PH (2017) Biostimulants in plant science: a global perspective. Front. plant sci. 7: 20-49.
Yu K, Soares JM, Mandal MK, Wang C, Chanda B, Gifford AN, Fowler JS, Navarre D, Kachroo A, Kachroo P (2013) A Feedback Regulatory Loop between G3P and Lipid Transfer Proteins DIR1 and AZI1 Mediates Azelaic-Acid-Induced Systemic Immunity. Cell Rep. 3: 1266-1278.
Zhao J, Davis LC, Verpoorte R (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv.. 23: 283-333. | ||
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